12
Setup
Setup
Setup
Setup
Setup
30.
Lean the microscope back to expose the lamp adjustment control located
on the bottom of the microscope stand taking care not to let the glass stage
plate and/or speciments fall. Loosen the transmitted lamp adjustment knob
located on the lamp compartment door by turning counter clockwise
(figure 40).
Figure 40
Figure 41
Figure 43
Figure 44
17
Setup
Setup
Setup
Setup
Setup
Kohler Illumination Setup
When using the transmitted illuminator proper condenser alignment, iris settings,
and focusing are necessary to obtain true Kohler illumination and achieve the
highest quality image of your specimen.
33.
Open the transmitted illuminator aperture and field diaphragms fully (figure
42), rotate the objective turret so that the 10X objective is in the optical path,
and focus on a specimen.
Transmitted Illumination Setup
(continued)
34.
While viewing through the microscope and slowly closing the field diaphragm,
adjust the substage condenser vertical control until the edge of the field iris
is in focus (figure 43).
35.
The two control knobs can be turned in unison or independantly to position
the condenser in alignment with the field diaphragm and light path (figure
44).
Continued on next page...
Reflected Illumination Setup
1.
Set the main power switch to the ON position (“|”) and the illuminator selector
switch to the UP position (“||”) (figure 15).
The reflected illuminator housing will get hot if left on for
extended periods of time. Be careful when making
adjustments to the illuminator housing or lamp fixture.
!
3.
Ensure that the illumination selector knob is pushed all the way inward (“R”)
(figure 17).
Figure 15
Figure 17
Figure 18
Figure 19
4.
Rotate the Aperture and Field diaphragm dials to the right (figure 18) to fully
open the iris’s.
5.
Rotate the color filter wheel until the blank position is in the light path
(figure 18).
6.
Pull the Polarizer filter slider out until you feel a click. Pull the Analyzer selector
knob fully out (figure 18).
7.
Rotate the 5X objective into the light path. You may have to lower the stage
in order not to crash the higher magnification objectives into the stage.
8.
Look through the eyepieces and you should see a white field of view. You
may have to adjust the illuminator intensity dial in case the light is too dim or
too bright. If the brightest portion of the field of view isn’t centered
(figure 19) you will need to perform the following instructions.
Continued on next page...
selector
switch
power
switch
selector
knob
analyzer
selector
iris dials
filter
wheel
filter slider
31.
Move the knob in latitudinal and/or longitudinal directions (figure 40) until the
field of view as seen through the eyepieces is at it’s brightest overall and the
brightest portion is well centered in the field of view (figure 41). This step
may be aided by placing a bright white piece of paper on the stage directly
under the objective and the light field viewed through that.
32.
Retighten the lamp adjustment knob by turning clockwise.
Figure 42
field iris
aperture
centering
controls
2.
Adjust the VLC (variable lighting control) (figure 16) counter-clockwise to
the maximum position.
Figure 16
VLC
adjustment
knob
