CID Gas (Triple Quad only)
Argon is recommended for use as a Collision Induced Dissociation gas for MS/MS in the triple
quadrupole MS, with a typical flow rate of 1 mL/min. The argon must have a purity of greater
than 99% and be regulated to 1
−
3 psi (0.1
−
0.2 bar).
Solvent Requirements 1200L LC/MS
Installation by a Varian Field Service Representative will normally include a basic test of the
system’s functionality.
Mobile phase solvents required for installation are methanol and de-ionized water. All solvents
should be pure HPLC-grade, and degassing apparatus should be available, if required.
You will need to provide the following glassware for diluting the standards used to mass
calibrate the instrument:
•
two 50 mL volumetric flasks
•
two 1.0 mL pipettes
In addition to the above, interconnect tubing will be required to complete installation of your
system. Most of this tubing is included in the accessory kits of specific LC instrument modules.
Additional tubing (PEEK® or Stainless Steel) may be required for installation of special valving
or installation of modules to existing HPLC systems.
For most analytical HPLC systems (flow rates up to 10 mL/min), 1/16" tubing with an internal
diameter (ID) of 0.005” can be used throughout. However, 0.005" ID or smaller should always
be used downstream of the sample injector or autosampler to prevent peak broadening. In
addition, tubing lengths should always be kept to a minimum.
Semiprep and Preparative systems have other requirements/limitations with regard to
interconnect tubing. Typically, a system using flow rates up to 200 mL/min will require 1/16" OD
tubing with an internal diameter as high as 0.040". On these systems, tubing ID used after the
injector/autosampler should be ~0.010" smaller than tubing used in the rest of the system. On
Prep systems, 1/8" tubing (0.062" or 0.080" ID) is typically used throughout. Always consult the
instrument module Operator’s Manual for specific recommendations.
1200L GC/LC/MS
Entrance
Before arranging for delivery of the 1200L GC/LC/MS to your facility, please make sure that all
passages to the site of installation are at least 36 in. (91.5 cm) wide. Allow additional room for
maneuvering the shipping containers around corners and/or through doors.
CAUTION
The 1200L MS, foreline pumps, GC, and LC are heavy items
that require at least two fit people for lifting and moving
safely.
Space and Load Requirements
The required layout for the 1200L MS with the HPLC is illustrated in Figure 4. The gas
chromatograph must be positioned to the left of the instrument to allow connection of the
transfer line from the GC to the MS. The 1200L MS uses two foreline pumps that should be
positioned on the floor beneath the workbench on which the 1200L MS system sits. There is a
96 in. (2.44m) vacuum line from the back, left of the MS that connects to the foreline pumps,
and a pump controller that must be positioned within 80 in. (2m) of the MS.
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1200/1200L MS Pre-installation Instructions
