Solvent Requirements 1200L LC/MS
Installation by a Varian Field Service Representative will normally include a basic test of the
systems functionality.
Mobile phase solvents required for installation are methanol and de-ionized water. All solvents
should be pure HPLC-grade, and degassing apparatus should be available, if required.
You will need to provide the following glassware for diluting the standards used to tune the
instrument:
•
two 50 mL volumetric flasks
•
two 1.0 mL pipettes
In addition to the above, interconnect tubing will be required to complete installation of your
system. Most of this tubing is included in the accessory kits of specific LC instrument modules.
Additional tubing (PEEK® or stainless steel) may be required for installation of special valving,
or installation of modules to existing HPLC systems.
For most analytical HPLC systems (flow rates up to 10 mL/min), 1/16" tubing with an internal
diameter (ID) of 0.005" can be used throughout. However, 0.005" ID or smaller should always
be used downstream of the sample injector or autosampler to prevent peak broadening. In
addition, tubing lengths should always be kept to a minimum.
Semiprep and Preparative systems have other requirements/limitations with regard to
interconnect tubing. Typically, a system using flow rates up to 200 mL/min will require 1/16" OD
tubing with an internal diameter as high as 0.040". On these systems, tubing ID used after the
injector/autosampler should be ~0.010" smaller than tubing used in the rest of the system. On
Prep systems, 1/8" tubing (0.062" or 0.080" ID) is typically used throughout. Always consult the
instrument module Operation Manual for specific recommendations.
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1200/1200L MS Pre-installation Instructions
