20
Installing a Gaseous Reagent
To install a gaseous reagent, do the following:
1. Connect the regulator of the gas cylinder to the back of the instrument using
a 4 mL/min restrictor.
2. See
the
4000 MS Ion Trap Hardware Operation Manual
for more details.
Auto Tune
Depending on your configuration and settings, you may not see all the available
Auto Tune routings. Perform auto tune after the instrument is first set up and
whenever significant maintenance operations are performed. Also, perform Mass
Calibration and Trap Frequency Calibration whenever the temperature or RF
adjustment is changed.
Auto Tune works the same way in either EI or Hybrid CI modes; you do not need
to run a different automatic setup, tuning, and calibration program for Hybrid CI.
Integrator Zero
Integrator Zero
obtains the average value of the signal level from the integrator
circuitry when the filament is off. When the filament is off, the major source of
signal coming is electronic noise. The integrator zero is adjusted so electronic
noise does not create artificial ions, which may create a measurable signal.
Set Electron Multiplier
Set Electron Multiplier determines two settings, the multiplier voltage needed to
achieve a multiplier gain of approximately 10
5
, and the Electron Multiplier voltage
boost for optimum peak intensity and resolution.
Electron Lens Tuning
Electron lens tuning involves measuring the transient behavior of the emission
current immediately after the lenses have been switched on or off. If the lenses
are unbalanced, the emission current will change in time and be proportional to
Summary of Contents for 4000 MS
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