Varian 900-LC Series, Getting Started Manual

Page 1: ...Varian 900 LC Series Liquid Chromatograph Getting Started Manual Installation Category II Pollution Degree 2 Safety Class 1 EN 61010 1 8510243600 December 2007 ...

Page 2: ...rnet home page can be found at www varianinc com Varian Australia Pty Ltd is the owner of copyright on this document and any associated software Under law the written permission of Varian Australia Pty Ltd must be obtained before either the documentation or the software is copied reproduced translated or converted to electronic or other machine readable form in whole or in part First published Dec...

Page 3: ...000 4 6 1996 Equipment Model Number Varian Model Instrument Type Authorized Representative in the EU Name G A Wassink Company Name Varian BV Signed Address Herculesweg 8 4330 EA Middelburg The Netherlands Position Quality Manager Telephone 31 0 118 671 000 Date 1 Month 20XX Facsimile 31 0 118 633 118 Manufacturer Name Phil Binns Company Name Varian Australia Pty Ltd Signed Address 679 Springvale R...

Page 4: ...Varian 900 LC Series Liquid Chromatograph This page is intentionally left blank 4 Publication date December 2007 ...

Page 5: ...due to Toxic Corrosive or Stimulative Solvent 14 Explosion of Vapor from Flammable Chemicals 14 Other Precautions 15 Warning and Caution Messages 15 Warning Symbols 16 Color Coding 17 US FCC Advisory Statement 17 Spurious and Harmonic Radiation Compliance 18 CE Compliance 18 1 Introduction 19 1 1 Pre installation Requirements 19 1 2 User Documentation 19 1 2 1 Conventions 19 1 2 2 Notes and Hot Ti...

Page 6: ...grated HPLC Online Help 40 2 4 Hardware Installation 41 2 5 Installation Qualification Tests 41 2 6 Customer Training 41 2 7 Using the Online Help 42 2 8 Instrument Maintenance Software IMS 42 2 8 1 How to launch IMS 42 2 8 2 About IMS 43 3 Operation 45 3 1 Analysis Checklist 45 3 2 Turning on the Instrument and Software 45 3 2 1 Connecting to a System 46 3 3 Preparing the System for Use 46 3 4 Co...

Page 7: ...Starting an Acquisition 52 3 6 1 Sequence 52 3 6 2 Quick Start 52 3 7 Printing a report 53 3 8 Saving Your Files 54 4 Maintenance 55 4 1 Routine Maintenance 56 4 2 Cleaning 57 4 3 Spare Parts 57 5 Troubleshooting 59 Publication date December 2007 7 ...

Page 8: ...Varian 900 LC Series Liquid Chromatograph This page is intentionally left blank 8 Publication date December 2007 ...

Page 9: ...e manufacturer the protection provided by the equipment may be impaired It is your responsibility to inform Varian representatives if the instrument has been used for the analysis of hazardous biological radioactive or toxic samples prior to any instrument service being performed or when an instrument is being returned to the service center for repair or is being recycled at end of life General Op...

Page 10: ...ials through a regulated disposal program and not through municipal sewage lines This instrument has been tested per applicable requirements of EMC Directive as required to carry the European Union CE Mark This equipment may be susceptible to radiation interference levels or frequencies which are not within the tested limits Electrical Hazards Exposure to high voltages and UV energy can cause seve...

Page 11: ...ith the same type and rating Make sure that voltage sources and line voltage match the value for which the instrument is wired Avoid using power supplies from a source that may be subject to electrical or RF interface from other services for example large electrical motors elevators and welders Compressed Gas Cylinders Unless your system is equipped with a helium sparging unit or an ELS detector t...

Page 12: ...ice which will discharge the gas at a predetermined temperature usually around 52 C 125 F If gases are to be plumbed from a remote storage area to the instrument site ensure that the local outlets are fitted with stop valves pressure gauges and suitable regulators which are easily accessible to the instrument operator The gas outlets must be provided within 1 5 metres 5 feet of the instrument High...

Page 13: ...LC system such that the concentration of ozone does not exceed the maximum permissible level All venting must be to outside air never within the building Flash Chromatography The operator should be familiar with the physico chemical properties of the components of the mobile phase Only use solvents compatible with the LC system tubing and fittings as certain solvents may cause weakening and leaks ...

Page 14: ...Solvent When using a toxic corrosive or stimulative solvent be careful not to incur a physical inflammation or injury For details of the properties of each solvent and how to handle it refer to the relevant Material Safety Data Sheets MSDS Be sure to handle each solvent properly Wear proper protective clothes e g safety goggles so that a solvent will not come into direct contact with the skin Vent...

Page 15: ...e cooling fans of the liquid chromatograph must be unobstructed Do not block the ventilation grills on the liquid chromatograph and accessories Consult the manuals supplied with your PC monitor and for their specific ventilation requirements Great care should be taken when working with glass or quartz parts to prevent breakage and cuts The 900 LC Series Liquid Chromatograph weighs approximately 14...

Page 16: ...ation or on the instrument itself are as follows Corrosive liquid Electrical shock Explosion hazard Eye hazard Fire hazard Heavy weight danger to feet Heavy weight danger to hands Hot surface Moving parts Radiation source The following symbol may be used on warning labels attached to the instrument When you see this symbol refer to the relevant operation or service manual for the correct procedure...

Page 17: ...s present A blue light indicates that operator intervention is required A red light warns of danger or an emergency US FCC Advisory Statement This equipment generates uses and can radiate radio frequency energy and if not installed and operated in accordance with the instruction manual may cause interference to radio communications It has been tested and found to comply with the limits for a Class...

Page 18: ...has confirmed that each product complies with the relevant Directives by testing a prototype against the prescribed EN European Norm standards Proof that a product complies with these directives is indicated by The CE Marking appearing on the rear of the product and The documentation package that accompanies the product containing a copy of the Declaration of Conformity The Declaration of Conformi...

Page 19: ...cement from your local Varian office Alternatively download a PDF from the Varian Inc Web site www varianinc com 1 2 User Documentation You have been provided with the following documentation to help you set up and operate your Varian Integrated Liquid Chromatograph This Getting Started manual with safety practices and hazards information where to find information about installing and maintaining ...

Page 20: ...Hot Tip is used to give practical hints to help you achieve the best possible performance from your instrument A Hot Tip is denoted by the following symbol 1 3 Specifications The instrument is suitable for indoor use only and is classified suitable under the following categories EN 61010 1 Installation category II Pollution degree 2 Safety class 1 1 3 1 Environmental Conditions Condition Altitude ...

Page 21: ...C System Power Connections Power supply phase Single Power supply current rating and overload protection Between 3 and 5 A for 220 240 V countries Between 6 and 12 A for 110 120 V countries Plug supplied Required wall socket Australia Complies with AS3112 To comply with AS3112 HPM 787 or Clipsal 2015 250 V 10 A USA Complies with Nema 5 15P To comply with Nema 5 15R Hubbell 5262 125 V 15 A Canada C...

Page 22: ...ach connection should be terminated at an appropriate receptacle within reach of each assembly s power cable Use of power boards or extension cables is not recommended It is recommended that separate mains circuits individually protected by fuses or circuit breakers are used for each component in the system such as the integrated liquid chromatograph PC and printer Avoid using power supplies from ...

Page 23: ...n 900 LC Series Liquid Chromatograph ELS Detector Helium may also be required to if you are using a helium sparging system to degas solvents Consult the documentation that came with your helium sparging system for gas requirements Nitrogen Gas Purity 98 filtered to 0 2 μm Permissible pressure range 60 to 100 psi 4 6 7 bar Maximum pressure 100 psi 6 7 bar regulated Required flow rates Up to 3 25 L ...

Page 24: ...nditions The area selected for the operation of a Varian LC system must be free from drafts corrosive atmospheres and vibration Sample preparation areas and materials storage facilities should be located in a separate room The area should be a dust free low humidity environment Air conditioning is strongly recommended for control of the environment For optimum analytical performance it is recommen...

Page 25: ...30 in 725 mm 29 in 665 mm 27 in 133 kg 294 lb Varian 940 LC Semi Preparative Liquid Chromatograph 750 mm 30 in 725 mm 29 in 665 mm 27 in 139 kg 307 lb Varian 940 LC Preparative Liquid Chromatograph 750 mm 30 in 725 mm 29 in 665 mm 27 in 137 kg 302 lb Shipping dimensions for all LC systems 950 mm 38 in 895 mm 36 in 1165 mm 46 in 170 kg 375 lb IBM compatible typical 450 mm 18 in 770 mm 30 in 520 mm ...

Page 26: ... you may want to use a PC you already have The recommended configuration should be followed when buying a new PC Minimum Recommended Pentium 4 processor 2 8 GHz or higher Core 2 Duo 2 13 GHz or higher 1 GB of RAM 2 GB of RAM 160 GB hard drive 2 x 200 GB hard drives 16 speed CD ROM or DVD RW 16 speed CD ROM or DVD RW Super VGA monitor with high color 16 bit display 1024 x 768 resolution minimum 17 ...

Page 27: ...0 64MB DDR NVidia GEForce4 440 MX Matrox G200 AGP 16 bit sound card 16 bit sound card Windows compatible keyboard Windows compatible keyboard Microsoft or compatible mouse Microsoft or compatible mouse Two PCI compatible slot for I O card Two PCI compatible slot for I O card Windows 2000 Windows XP SP 1 Microsoft Internet Explorer v6 0 Microsoft Internet Explorer v6 0 Table 6 Recommended PC specif...

Page 28: ...Varian 900 LC Series Liquid Chromatograph This page is intentionally left blank 28 Publication date December 2007 ...

Page 29: ...nstrument and what to do in case it has been damaged in transit are also outlined in the pre installation manual Basic installation involves Setting up the PC to instrument communications Installing the Galaxie software Installing the Online Help Installing the hardware Running the Installation Qualification tests Basic customer training 2 1 900 LC Series LC System to PC Communications The company...

Page 30: ...guration Figure 2 Multiple 900 LC Series LC Systems in standalone configuration Up to four systems can be controlled by one Galaxie Acquisition Server For a client server system there will need to be a PC with the Galaxie Acquisition Server running for every four HPLC 30 Publication date December 2007 ...

Page 31: ...e 900 LC Series LC System configuration option 1 Figure 4 Networked multiple 900 LC Series LC System configuration option 2 You or your network administrator must provide any hardware adapters cables and configuration and setup work required to Publication date December 2007 31 ...

Page 32: ...LC system 2 Connect an Ethernet cable from the Ethernet switch to the Ethernet port on the PC s and or company network 2 2 Installing the Galaxie Chromatography Data System Varian recommends that you purchase a PC as part of the Varian 900 LC Series Liquid Chromatograph package The PC included in the package will come with the appropriate operating system as loaded by the PC supplier For instructi...

Page 33: ...s using up to three PCs Main server where data and methods are stored Acquisition server communicates with the hardware of the 900 LC Series HPLC system Up to four 900 LC systems can be run by one acquisition server The system is configured on the acquisition server Client methods are created data viewed and reprocessed Note The 900 LC Series driver must be installed on all three PCs The online he...

Page 34: ... firewall on the PC s to be used with Galaxie software This will help prevent installation and data collection problems Prior to installation check the following Disable Hyperthreading in the Bios Consult your PC manual for instructions Check that power management of your Ethernet network card is set to never allow the card to be turned off Consult your PC manual for instructions In the Windows Co...

Page 35: ...up includes having the network cabling and hardware plugged in To install the 900 LC Series HPLC Driver 1 Insert the 900 LC Series Galaxie Driver CD into your CD ROM drive The software will automatically start1 2 Click Next on the Welcome to Install Shield Wizard for Varian Series 900 LC Drivers window 3 Click Yes to accept to the License agreement Clicking no will terminate the program 4 Enter in...

Page 36: ...tructed Click OK If you click Cancel a message will appear confirming your choice not to install the Online Help Click No to exit the installation or click Yes to continue installing the Online Help 2 Click OK to continue the installation 3 Click Next on the Welcome to the Varian 900 LC Series HPLC Help Setup Wizard window 4 Click OK to accept the default installation location or choose a differen...

Page 37: ...ve typed in the correct system name To modify a system name later you must first delete that LC system and then use this SetupWizard exe to recreate the LC system 5 Click on the magnifying glass icon to start the system discovery process This can take up to 5 10 seconds 6 Click the down arrow button in the Instrument field to select the LC system Note Identify the system by the MAC address which c...

Page 38: ...hen the message confirming the system creation comes up 10 Create up to three more systems or click Close to exit the installer 2 2 4 Delete a System If the system needs to be renamed or the IP address changed you will need to delete the existing system and recreate it To delete a system 1 Open Galaxie Configuration Manager 2 Click the Windows Start button and choose Programs Galaxie Galaxie Confi...

Page 39: ...xe To change the name or IP address of a system 1 Delete the LC system See page 38 for instructions 2 Re create the LC system See page 36 for instructions 2 2 6 Update Galaxie to Version 1 9 SP1 The 900 LC Series HPLC Driver requires Galaxie version 1 9 sp1 to run The ordering information for the CD is part number 03 950000 98 Galaxie Software Service Pack 1 SP1 CD Note Contact your local Varian r...

Page 40: ... made to the Varian 900 LC Series LC online help or Galaxie Chromatography Data Systems help The Varian LC system online help is provided on a separate CD to the Galaxie software and is installed on your PC during the Galaxie software installation The LC system help includes information and instructions on Safety LC system standard and optional hardware Installation of the LC system options and co...

Page 41: ...instrument connections including connecting the network and communication cables please refer to the online help 1 Click the Windows Start button and choose Programs Galaxie Help Varian Integrated Help 2 When the Integrated Help appears click About Your Instrument link to access information about the front side and back connections and components of your LC system 2 5 Installation Qualification Te...

Page 42: ...rocedures that can be tracked The Log Book lists all actions and counter information and can be saved in a PDF file Service tests which can test the performance of devices in the LC system 2 8 1 How to Access IMS Access the Instrument Maintenance Software from the link on the right side of the Status Overview window in the Galaxie software The Instrument Maintenance software can be run with the Ga...

Page 43: ...ons Maintenance mode ON Maintenance mode OFF About For detailed information about the IMS software please see the 900 LC Series HPLC online help To access the online help 1 Click the Windows Start button 2 Click Programs 3 Click Galaxie 4 Click Help 5 Click Varian Integrated Help 6 Click Instrument Maintenance Software in the online help table of contents For information on audit trails 1 Click th...

Page 44: ...s Liquid Chromatograph 4 Click Help 5 Click Varian Integrated Help 6 Click the plus sign next to Instrument Maintenance Software in the online help table of contents 7 Click Audit Trails 44 Publication date December 2007 ...

Page 45: ... You need to complete the following steps in turn to measure a sample s You will find details on each step in this chapter Turn on the instrument and software Prepare the instrument for use Configure the Device Settings and System Parameters Create a method Run samples View or Print a report 3 2 Turning on the Instrument and Software Before starting the system carefully read the Safety practices a...

Page 46: ...ting with your LC system Note There will be a delay between choosing your system and the system information appearing on your screen The Galaxie software is communicating with the LC system 3 3 Preparing the System for Use For consistent and reproducible results with each use of the iHPLC it is recommended that you follow this procedure to prepare your system to run samples To prepare the system f...

Page 47: ...g with a non absorbing solvent flowing through the cell with no air bubbles present For more information on getting your system prepared for use please see the 900 LC Series HPLC Help 1 Click the Windows Start button 2 Click Programs 3 Click Galaxie 4 Click Help 5 Click Varian Integrated Help 6 Click the plus sign next to Getting Started 7 Click Preparing the LC System For Use 3 4 Configuring the ...

Page 48: ...purge the pumps set the bottle management parameters select the solvents for the scale up module valves if present in your system 3 4 2 Detectors Depending on the detector you purchased in the detector Device Settings you can choose wavelengths autozero the detectors turn your detector lamp s on choose the data collection parameters set the detector temperature conditions 3 4 3 Column Depending on...

Page 49: ...sh Syringe Barrel button If you have the cooling option on your autosampler you can change the temperature Manual Injector Depending on the options you chose you may have a manual injector This allows you to make single injections The Device Settings window lets you make an injection and shows you the state of the injector Injection Pump The preparative scale LC system may have an injection pump I...

Page 50: ... to be used in the valve module section if you have a Scale Up Module with semi prep and prep systems you can choose the pump head sizes sample delivery pump head size and pressure units in the fraction collector section you must choose your rack type tube volume and probe depth save your settings 3 5 Creating a Method To create a new method 1 Click File New Method in the upper left corner of the ...

Page 51: ...0 In the Acquisition Parameters section enter in the Vial and Rack number if an autosampler is to be used the Acquisition length and Injection Volume Note For information about the additional fields in the Acquisition window please see your Galaxie Chromatography Data System software Online Help 11 Click File Save Save Method The method will now be saved in the default data folder For more informa...

Page 52: ...ou chose in step 3 You can auto fill the columns by entering or choosing a value in the first line and then right clicking the heading of that column Choose Fill Block and then follow the prompts Alternatively you can manually fill in each field to customize your sequence 5 Enter in all required fields 6 Click File Save Save Sequence 7 On the Sequence page click the green arrow button just above a...

Page 53: ...he Galaxie software Note For further information about running samples and interpreting the results refer to the Galaxie Chromatography Data System Online Help 3 7 Printing a report To print a report 1 Click Report Style in the lower left corner of the Galaxie software Note When printing a report you can simply select Print from the File menu However if you do so a report will be generated based u...

Page 54: ...g the previous one by using the menu File Save Save file type Save the file with another name by using the menu File Save As Save file type As In both cases the Galaxie Software prompts as default to save the file type corresponding to the active file The Save file window displays the list of the files already created in the directory associated with the group when the user is logged on to All pro...

Page 55: ...es Warning The UV Vis PDA and Fluorescence detector lamps are intense light sources Direct viewing of the light source will cause eye damage Operators and other unauthorized personnel must NEVER remove the main covers Warning This instrument contains electrical circuits devices and components operating at dangerous voltages Contact with these circuits devices and components can result in death cau...

Page 56: ... Provide sufficient ventilation in the room Use of the LC system and accessories may involve materials solvents and solutions which are flammable corrosive toxic or otherwise hazardous Careless improper or unskilled use of such materials solvents and solutions can create explosion hazards fire hazards toxicity and other hazards that can result in death serious personal injury or damage to equipmen...

Page 57: ...ed up immediately The exterior surfaces of the instrument should be kept clean All cleaning should be done with a soft cloth If necessary this cloth can be dampened with water or a mild detergent Do not use organic solvents or abrasive cleaning agents 4 3 Spare Parts For spare parts and consumables ordering information refer to the Varian Inc Web site www varianinc com Publication date December 20...

Page 58: ...Varian 900 LC Series Liquid Chromatograph This page is intentionally left blank 58 Publication date December 2007 ...

Page 59: ...s the online help troubleshooting section 1 Click the Windows Start button 2 Click Programs 3 Click Galaxie 4 Click Help 5 Click Varian Integrated Help 6 Click the Troubleshooting link for general LC system troubleshooting or click the plus sign next to Troubleshooting to access the device troubleshooting information Publication date December 2007 59 ...