72
03-914648-00:7
Temperature
Ramp
Splitless
Mode
The splitless temperature ramp mode is preferred for compounds that are
altered by higher temperatures (thermolabile). Also, the splitless temperature
ramp mode is used with wide boiling range mixtures (e.g., hydrocarbon
mixtures). Two glass inserts are recommended when operating in the
splitless temperature ramp mode: 1) The 2 mm ID glass wool packed insert
is used for non-polar compounds at levels
>
1 ng; and 2) the 0.5 mm ID open
insert is used for thermolabile and/or polar compounds at trace levels (pg
level).
In the splitless temperature ramp mode, the injector is held at a temperature
that is equal to or slightly below the boiling point of the injection solvent. The
sample is deposited on the surface of the insert. After injection, the
temperature of the injector is increased rapidly (ramped). As the temperature
of the injector increases, the sample then vaporizes and is swept onto the
column.
On-Column
Modes
In the on-column modes the column is sealed to the glass insert (0.18 - 0.53
mm columns). This ensures that there is maximum transfer of sample to the
column. In the on-column modes the sample is normally injected at or below
the solvent boiling point and then the injector is ramped to transfer the
sample onto the column.
Large
Volume
In the large volume mode of injection typically > 5 µL of sample is deposited
into the injector slowly, the solvent is vented and then the components of
interest are transferred to the column. This is done using a special split vent
program and an injector temperature ramp. The 8200 AutoSampler allows
automated injection of up to 100 µL of sample.
Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com
