90
03-914648-00:7
Hang the coiled column on the capillary column holder then prepare column
end and complete column connections to both the injector and detector
fittings, as detailed in the following paragraphs.
Preparing
Column
Ends
To ensure a leak-free connection of capillary columns, the ends of the
column must be cut squarely and as smoothly as possible. To achieve this, a
proper scoring tool is required. A ceramic scoring wafer (01-900158-00) is
recommended. Before cutting the sealed ends of the column, slide the
capillary column nut over the column end. Install the appropriate Vespel,
graphite/Vespel, or graphite ferrule onto the column with the tapered end
toward the capillary column nut. This procedure prevents contaminating the
column with ferrule material.
1
Grasp the column securely between the thumb and forefinger of
one hand. Score the column once lightly with the cutting tool.
2
Per Figure 10, bend column slightly to break it at the score mark.
A magnifying lens (recommended 20X magnifier, 00-997369-00) is
necessary to determine the quality of the cut. (If small splinters of
silica or some of the outer coating remains on the column end, it is
advisable to make a fresh cut.)
Figure 10 Column Ends
1
Capillary column nut
2
Fused silica column
3
Capillary column ferrule
4
Properly cut column end
5
Improperly cut column ends
Column Ferrule Selection Table
Ferrule ID
Column ID
0.4 mm
250µ
0.5 mm
320µ
0.8 mm
530µ
3
1
4
5
2
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