ProStar 340 UV-Vis Detector
17
Operation
Introduction
Turn on the HPLC system and allow the column to equilibrate
with the flowing eluant (the time required will depend upon your
particular column and eluant). If you have not already done so,
turn ON the power to the detector using the switch located on
the lower rear panel.
Turn the Range selector knob to 2.0 AUFS and Rise Time
selector to 1.0 sec. Adjust the wavelength drive to the
appropriate wavelength for your test mix.
During the lamp ignition period, adjust the chart recorder pen to
your desired position. After lamp ignition, the appropriate lamp
indicator (UV for D2, Vis for Tungsten) will light, an absorbance
will be shown on the LED display, and the Wait Light will still be
lit. During this interval, the recorder output will be fixed at zero
volts while the integrator output will transmit a voltage related to
the present flowcell absorbance and last zero value stored in the
autozero circuit memory. To activate the recorder output, press
the autozero switch.
NOTE:
When the unit is first switched on, the display will show random values,
typically 1 or -1. The Wait LED will be ON. The D2 lamp ignites in
approximately 20 seconds; the tungsten lamp ignites immediately.
This feature protects the chart recorder from rapidly moving and
slamming into its margin if the detector is inadvertently set at a
sensitive range and a large discrepancy exists between flowcell
absorbance and the last stored zero value.
After the lamp has ignited and the autozero button has been
pushed, it will be necessary to push the Short Switch to set the
Summary of Contents for ProStar 340
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