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Flow Cell Unit Maintenance
Checking and Cleaning the Flow Cell
If the inside of the flow cell is contaminated, it must be cleaned.
The following items are required for cleaning the flow cell.
•
A glass syringe with capacity of approximately 10 mL
•
Appropriate washing solution (step 5).
1. Loosen the clamp screw retaining the flow cell unit.
2. Remove the flow cell.
3. Check the inside of the cell to see if it is contaminated with
any foreign substance (e.g. oils, salts or buffers).
4. Check the outside of the cell for leakage.
5. If any contamination is observed inside the cell, it must be
cleaned with an appropriate solvent.
Connect the syringe to the tip of drain tube, and inject the
washing solvent into the cell for cleaning.
If an aqueous mobile phase is used, water should be used.
If an organic based mobile phase is used, methanol or
acetonitrile should be used.
6. Replace the washing solvent with the eluent that is used for
the separation and repeat the process indicated in step (5).
7. If air bubbles are observed in the cell, deliver mobile phase
into the flow cell with the pump using a flow rate of less than
1.0 mL/min and a pressure of less than 150 psi (10 atm).
While the liquid is being delivered to the flow cell, pinch the
end of the drain tube for a few seconds to increase the
pressure inside the cell. Repeat this procedure three or four
times until all air bubbles have been removed.
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