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4. Observation Methods
4-1 Reflected Light Brightfield/Darkfield Observation
The following flow shows the basic operating procedure for reflected light brightfield or darkfield observation.
1.
Select the brightfield (BF) or darkfield (DF) observation (see Mirror selector lever)
2.
Set the main switch to “I” (ON)
2.1.
Disengage the analyser, polariser, filter, etc. from the light path.
2.2.
Check interlocking of the ND filter
3.
Select the light path
4.
Place the specimen on the stage.
5.
Engage the 10X objective in the light path.
6.
Bring the specimen into focus.
6.1.
Adjust the brightness.
6.2.
Adjust the interpupillary distance.
6.3.
Adjust the dioptre.
7.
Adjust the aperture iris diaphragm and field iris diaphragm.
Open both iris diaphragms in case of DF observation.
8.
Start observation.
