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Concentration measurement
Set up linear regression curve
There are two methods available to set up the linear regression curve. You can use standards to set up the
regression curve or just enter the parameters manually. Use the following steps to select the method you
wish to use.
1. Click
on the toolbar.
2. Click
the
Method
tab.
3. Enter the number of wavelength points in the
Number of Points
box, or click the
up/down
arrow next
to this box. With two wavelengths, the absorbance at the second reference wavelength is subtracted
from the first to correct for background absorbance. With three wavelengths, the baseline between the
first and third wavelengths is calculated and its value at the second wavelength is subtracted from the
absorbance at the second wavelength to give the peak height.
4. Enter the wavelengths in the
Wavelength
boxes.
5. Tick
the
Calculate Concentration
tick box to activate concentration calculation.
6. Set up the linear regression curve.
Method 1: Set up the linear regression curve with prepared standards.
(1) Tick
the
Use Standard Samples
tick box.
(2) Place the reference into the reference holder.
(3) Click
the
Standard
tab.
(4) Place Standard 1 in the sample compartment. Click
Start
to run a measurement.
(5) Enter the concentration value of Standard 1 in the
Conc.
box.
(6) Enter the sample name for the standard in the
Name
box.
(7) Click
OK
. The photometric data,
△
A and concentration will be shown in the standard table.
