Instruction manual |
25
12. Fluorescence observation (reflected light)
(Used commands)
(Chapter)
Set the main swith to (ON) and adjust light intensity.
Place a specimen on the stage.
Insert in the light path the filtercube
suitable for the specimen
Begin observation
Insert the objective into the light path
and focus the specimen.
Insert the anti-glow cap to reduce flares
Main switch
Anti-glow cap
13
13
Fluorescence filter slider
13
Nosepiece
Coarse and fine focusing knobs
7
7
Stage
10
Brightness adjustment knob
13
13. Use of the microscope in fluorescence
(reflected light)
13.1 Using the microscope
Move the main switch in the position “I” to turn on the
microscope, then rotate the adjustment knob (1) (Fig. 40)
to
adjust the fluorescence light intensity.
13.2 Switching fluorescence filter cubes
Move the filter lever (placed on the left side of the microscope)
(2)
to insert the desired filter cube (see tables below). (Fig. 41)
13.2.1 Available fluorescence filter cubes
IT406 FL2 BG2
1
2
FIGURE 40
FIGURE 41
Filter
name
Excitation
filter
Dichroic
mirror
Barrier
filter
Applications
B
460-490 nm
495 nm
520LP nm
–
FITC: fluorescent antibodies
–
Acridine orange: DNA, RNA
–
Auramine
G
540-580 nm
585 nm
590LP nm
–
Rhodamine, TRITC: fluorescent antibodies
–
Propidium iodide: DNA, RNA
–
RFP
