VWR TL534B FL BG, Instruction Manual

Page 1: ...ean Catalogue Number TL534B FL BG 630 3270 TL534B FL4 630 3243 TL534B SA FL4 630 3244 VisiCube TL_B 630 3245 VisiCube TL_G 630 3246 VisiCube TL_V 630 3247 VisiCube TL_UV 630 3248 VisiCube TL_R1 630 3249 VisiCube TL_R2 630 3250 VisiCube TL_R3 630 3251 VisiCube TL_FR 630 3252 VisiCube TL_A 630 3253 ...

Page 2: ... Europe VWR International bv Researchpark Haasrode 2020 Geldenaaksebaan 464 B 3001 Leuven 32 16 385011 be vwr com UK Importer VWR International Ltd Hunter Boulevard Magna Park Lutterworth Leicestershire LE17 4XN uk vwr com Country of Origin Italy ...

Page 3: ... 5 Diopter adjustment 13 9 6 Adjusting the interpupillary distance 13 9 7 Light path selection 13 9 8 Use of eye shields 13 9 9 Centering the condenser 14 9 10Effects of the field diaphragm 14 9 11 Aperture diaphragm 14 9 12Use of oil immersion objective 15 9 13Use of the polarizer optional 15 10 Fluorescence observation procedures reflected light 16 11 Use of the microscope reflected light 17 11 ...

Page 4: ...plying voltage of your region matches with the operation voltage of the equipment and that the lamp switch is in off position Users should observe all safety regulations of the region The equipment has acquired the CE safety label However users have full responsibility to use this equipment safely Please follow the guidelines below and read this manual in its entirety to ensure safe operation of t...

Page 5: ...scription PHOTO TV PORT DIOPTER ADJUSTMENT RING NOSE PIECE MAIN SWITCH TRANSMITTED LIGHT ADJUSTMENT KNOB OBSERVATION HEAD CONDENSER HEIGHT ADJUSTMENT KNOB EYEPIECES CONDENSER CONDENSER CENTERING SCREWS SLIDE HOLDER COARSE FOCUS KNOB COARSE UPPER LIMIT KNOB FINE FOCUS KNOB ...

Page 6: ...n manual 6 Opposite side APERTURE DIAPHRAGM X Y MOVEMENT KNOBS OBJECTIVES FIELD DIAPHRAGM STAGE LIGHT PATH SELECTOR LEVER FLUORESCENCE CUBE SELECTOR LEVER UV SHIELD REFLECTED LIGHT ADJUSTMENT KNOB TENSION ADJUSTMENT RING ...

Page 7: ...ch with bare hands optical surfaces such as lenses filters or glasses Traces of grease or other residuals may deteriorate the final image quality and corrode the optics surface in a short time 7 Assembling Once opened the box the microscope parts are the following 1 Microscope frame 2 Eyepieces 3 Objectives TL534B FL BG TL534B FL W PLAN series TL534B SA FL4 W PLAN F series 4 Observation head 5 Epi...

Page 8: ...luminator Fig 2 3 Insert the optical head above the epi illuminator and tighten the screw 4 Fig 3 Hold the head with one hand during the locking in order to avoid that the head falls 4 Insert both eyepieces into the tubes of the optical head Fig 4 Condenser is pre installed in the factory To remove the condenser use an Allen wrench 1 5 mm diam and operate on the locking screw placed on the right s...

Page 9: ...TL534B FL BG model comes with B and G cubes preinstalled 1 Disconnect the power supply plug from the fluorescence illuminator 2 Open the side cover of the illuminator by unscrewing the side screws 1 Fig 7 It could be helpful to remove the observation head The cubes are mounted on the opposite side of the cover opening the left cover acts on the right side of the slider and vice versa 3 Open the to...

Page 10: ... fluorescence filter 1 Disconnect the power supply plug from the fluorescence illuminator 2 Open the side cover of the illuminator by unscrewing the side screws 1 Fig 7 It could be helpful to remove the observation head The cubes are mounted on the opposite side of the cover opening the left cover acts on the right side of the slide and vice versa 3 Open the top door of the fluorescence illuminato...

Page 11: ...it 1 at the base of the microscope Fig 13 2 Loosen the head fixing knob 2 and remove the head from the microscope frame Fig 14 3 Insert the analyzer into the hole inside the frame 3 Fig 15 4 Put back the head into its original position and lock the fixing knob FIGURE 14 2 FIGURE 15 3 FIGURE 13 1 ...

Page 12: ... path the desired objective and focus the spec imen Adjust interpupillary distance Adjust diopter Center optical axis Adjust brightness 8 Brightfield observation procedures transmitted light Main switch brightness adjustment knob Nosepiece Observation head Diopter adjustment ring Condenser centering Aperture diaphragm Field diaphragm Nosepiece Light adjustment dial Coarse and fine focusing knobs C...

Page 13: ...een slide and objective and acts as focus memory 1 After focussing the specimen pull the lever 3 toward the front of the microscope and lock it Fig 18 In this way the focus upper limit is set 2 Now one can lower the stage with coarse focus knob replace the specimen and raise again the stage up to the upper limit specimen will be in approximate focus and will need a fine adjustment to get the prope...

Page 14: ...eir common axis until the fields of view coincide The graduation on the interpupillary distance indicator 2 pointed by the spot on the eyepiece holder shows the distance between the operator s eyes Fig 21 The range of the interpupillary distance is 48 75 mm 9 7 Light path selection The observation head is equipped with an optical path selector that allows the light to be split to the eyepieces and...

Page 15: ...ew 7 In normal use open the diaphragm until it circumscribes the field of view 9 10 Effects of the field diaphragm Field diaphragm adjusts the illuminated area to obtain a high contrast image Set the diaphragm according to the objective in use until it circumscribes the field of view in order to eliminate unnecessary light to eyepieces Fig 26 9 11 Aperture diaphragm The Numerical Aperture N A valu...

Page 16: ... immersion objective 5 Return the stage to the upper focusing point and obtain an optimal focus using the fine focus knob 6 After use gently remove the oil with a soft paper towel or a lightly moistened optic paper with a mixture of ethyl ether 70 and absolute ethyl alcohol 30 The immersion oil if not immediately cleaned could crystallize creating a glass like layer In this situation the observati...

Page 17: ...scence filter turret Nosepiece Fluorescence main switch Coarse and fine focusing knobs Turn the fluorescence main switch to turn on the LED Put a specimen on the stage Insert in the light path the filter suitable for the specimen Begin observation Insert desired objective for the observation and focus the specimen Adjust light intensity 11 9 11 5 5 11 ...

Page 18: ...he filter selector 2 in the desired position Fig 31 2 When the filter is in the click stop position the dedicated LED will turn on When switching the fluorescence filter the LED light goes out This is not a defect 1 2 Filter name Excitation filter Dichroic mirror Barrier filter Applications VisiCube TL_UV 325 375 nm 415 nm 435LP nm DAPI Hoechst VisiCube TL_V 390 420 nm 440 nm 450LP nm Coumarin Pac...

Page 19: ...tly over the plate Fig 32 Mode n 2 lower the condenser and insert the plate between the two layers of the stage Fig 33 In both cases it is possible to move the sample using the stage X Y translation knobs 11 4 Use of UV shield Microscope is provided with a UV protection shield This can be used to protect user from unwanted UV rays coming from the fluorescence light source 1 Loosen the two locking ...

Page 20: ...lex adapter 3 Connect the Reflex camera 4 to the T2 just installed Fig 38 4 Mount the other end of the relay tube 2 into the empty hole of the trinocular port then tighten the clamping screw Fig 36 T2 ring is not provided with the microscope but is commercially available While shooting dark specimens darken eyepieces and viewfinder with a dark cloth to minimize the diffused light To calculate the ...

Page 21: ... One side of the image is out of focus The nosepiece is not in the center of the light path Turn the nosepiece to a click stop The specimen is out of place tilted Place the specimen flat on the stage Stage is not correctly mounted Re attach it The optical performance of the sample cover glass is poor Use a cover glass of better quality Image appears to waver Nosepiece is not corrected mounted Push...

Page 22: ...roscope to manufacturer for maintenance please use the original packaging 15 Technical service Web Resource Visit the VWR s website at www vwr com for Complete technical service contact information Access to VWR s Online Catalogue and information about accessories and related products Additional product information and special offers Contact us For information or technical assistance contact your ...

Page 23: ...g It is also your responsibility to decontaminate the equipment in case of biological chemical and or radiological contamination so as to protect from health hazards the persons involved in the disposal and recycling of the equipment For more information about where you can drop off your waste equipment please contact your local dealer from whom you originally purchased this equipment By doing so ...

Page 24: ...5 14 85 00 international info fr vwr com 0 18 TTC min prix appel POLAND VWR International Sp z o o Limbowa 5 80 175 Gdansk Tel 48 58 32 38 200 info pl vwr com PORTUGAL VWR International Material de Laboratório Lda Centro Empresarial de Alfragide Rua da Indústria nº 6 2610 088 Amadora Tel 351 21 3600 770 info pt vwr com SPAIN VWR International Eurolab S L U C Tecnología 5 17 A 7 Llinars Park 08450 ...