Instruction manual |
21
13. Troubleshooting
Review the information in the table below to troubleshoot operating problems.
Problem
Cause
Solution
I. Optical System:
LED does not light
Power supply is unplugged
Connect into the power outlet
LED operates, but field of view remains dark
Brightness is too low
Set brightness to a proper level
Fluorescence filter selector is not in a click stop
Move the selector to a click stop
Fluorescence filter is not suitable for the specimen
Use a suitable filter
Light path selector knob is set to the camera position
Move the knob to the eye position
Field of view is obscured or not evenly illuminated
Light path selector knob is in an intermediate position
Set the knob according to the observation method
Nosepiece is not correctly engaged
Make sure that the nosepiece clicks properly into place
Condenser is not attached properly
Re-attach it
Nosepiece is not attached properly
Push the side dovetail all the way until it is stopped
An objective that falls outside of the condenser’s illumination
range is used
Use a condenser to match the purpose
Condenser is not properly centered
Center the condenser
Field diaphragm is stopped down too far
Open the field diaphragm until it circumscribes the field of view
Fluorescence filter selector is not in a click stop
Move the selector to a click stop
Dirt or dust is visible in the field of view
Dirt/dust on the eyepieces
Clean thoroughly
Dirt/dust on the condenser surface
Dirt/dust on the specimen
Image looks double
Aperture diaphragm is stopped down too far
Open aperture diaphragm
The condenser is not well centered or it is in a wrong height
Set the condenser according to Koehler settings
Low image quality
‒
Image is not sharp
‒
Contrast is poor
‒
Details are indistinct
‒
Image glares
Condenser is lowered too much
Adjust the condenser height position
Aperture diaphragm is stopped down too far
Open aperture diaphragm
Nosepiece is not mounted properly
Push the slide dovetail all the way until
it is stopped
Immersion oil is not being used with an oil immersion objective
Use immersion oil
Immersion oil contains bubbles
Remove the bubbles
Recommended immersion oil is not used
Use the provided immersion oil
For transmitted light observation, coverglass thickness must not
exceed 0.17 mm
Use a coverglass with thickness 0.17 mm
One side of the image is out of focus.
The nosepiece is not in the center of the light path
Turn the nosepiece to a click stop
The specimen is out of place (tilted)
Place the specimen flat on the stage.
Stage is not correctly mounted
Re-attach it
The optical performance of the sample cover glass is poor
Use a cover glass of better quality
Image appears to waver
Nosepiece is not corrected mounted
Push slide dovetail all the way until it is stopped
Objective is not correctly engaged in light path
Make sure that nosepiece clicks into place correctly
Condenser is not properly centered
Center the condenser
Field of view becomes only slightly brighter when the voltage
is raised.
Condenser is not properly centered
Center the condenser
Condenser is lowered too far
Adjust the condenser height position
II. Mechanical Section:
Coarse adjustment knob is hard to turn
Tension adjustment ring is too tight
Loosen tension adjustment ring
You are trying to raise stage while focus-lock lever is kept locked Unlock focus-lock lever
Stage drifts down by itself or focus is lost during observation
Tension adjustment ring is too loose
Tighten ring
Coarse adjustment will not go all the way up
Focus-lock lever is locked at a too low height
Unlock focus-lock lever
Coarse adjustment will not go all the way down
Condenser holder is too low
Raise condenser holder
Objective makes contact with specimen before focus is
obtained
Specimen is mounted upside down
Mount specimen correctly
III. Electrical Section:
LED doesn’t turn on
Power supply not connected
Check for proper connection
Brightness is not enough
Brightness setting is too low
Adjust brightness
Light blinks
Power supply not well connected
Check for proper connection
IV. Observation tube:
The field of view of one eye does not match the field of view of
the other eye
Interpupillary distance is incorrect
Adjust interpupillary distance
Incorrect diopter adjustment
Adjust diopter
Your view is not accustomed to microscope observation
Upon looking into eyepieces,
try looking at overall field before
concentrating on specimen range.
You may also find it helpful
to look up and into distance for a moment before looking back
into microscope
V. Microphotography:
Image edge is unfocused
To a certain extent it is due to achromatic objectives features
To minimize the problem,
set the aperture diaphragm in a
proper position
Bright spots appear on the image
Stray light entering in the microscope through eyepieces or
camera viewfinder
Cover eyepieces and viewfinder with a dark cloth
