2-12
Preparing System Hardware
Preparing the sample manager
Prepare the sample manager for operation after you prepare the solvent
managers. Preparing the sample manager involves these steps:
•
Priming the sample and wash syringes
•
Characterizing the seal
•
Characterizing the needle and sample loop volumes
•
Loading sample plates
Caution:
To avoid solvent spills and maintain proper leak drainage, always
close the sample manager fluidics tray before operating the system.
Tip:
For venting purposes, the sample manager and sample organizer fans are
always operating.
Selecting weak and strong wash solvents
Tip:
For best performance, follow these guidelines when selecting wash
solvents. Otherwise, performance can be reduced, specifically Area/Height
RSD and Linearity. This does not mean that all other solvent combinations
are prohibited. Other combinations can be run with lower performance
expectations or by manipulating default injection parameters.
Use a weak wash solvent based on the sample and mobile phase chemistries of
your application, making sure all solutions and buffers are miscible and
soluble.
Caution:
The nanoACQUITY UPLC System should not be run with high pH
mobile phases. Alkaline solutions such as ammonium hydroxide (pH 10) can
etch glass solvent bottles and the silica capillary tubing, resulting in an
elevated chemical background being detected by mass spectrometry.
Warning:
To avoid electric shock, power-off and unplug the sample
manager before performing any maintenance operation such as
replacing fuses.
Summary of Contents for nanoACQUITY UPLC
Page 7: ...vii ...
Page 16: ...xvi ...
Page 20: ...xx Table of Contents ...
Page 32: ...1 12 System Overview ...
