YMC Europe GmbH
Troubleshooting
YMC Pilot
42 / 49
Low pressure chromatography column
8
Troubleshooting
WARNING
Incorrect maintenance
Severe injuries during operation after incorrect maintenance possible if the column or
column parts do not withstand the operating pressure.
► Only authorised and qualified personnel should carry out the maintenance.
WARNING
Incorrect replacement of spare parts
Severe injuries due to breakage possible, if authorised spare parts are not used.
► Use only the manufacturer authorised parts.
Fault
description
Possible cause
Solution
Dead volume
occured
Exceeding the maximum
allowable pressure of the
column packing
► If dead volume occurs, proceed
as follows:
1. Stop the pump.
2. Readjust the piston of the
adjustable end piece.
Stressed column packing
Peak shape of the
eluted substances
deteriorates
Dead volume at the column
inlet
See fault description “Dead
volume occurred“
Partially clogged frit
Frit replacement Chapter 7.4, p.
40.
Separation performance of
the stationary phase altered
by impurities
Wash the stationary phase.
Contact also the manufacturer of
the packing material.
Stationary phase
mechanically destroyed
Replace packing material
Air in the column
Degassing or evaporation of
solvent during storage
New column conditioning.
Possibly new packing material is
necessary.
Exceptional
pressure rise during
operation
Valve circuit incorrect
Check valve positions
Clogged frit
Frit replacement Chapter 7.4, p.
40.
Over-tightened fixing screws Replace fixing screw and
clamping tubing, cut the end of the
tubing.
Pressure drop
during operation
Leakage from tubing or fitting
between pump and column
Check tubing and connections
Empty solvent reservoir
Refill solvents
