Zeiss Axio Examiner, Operating Manual

Page 1: ...Operating Manual Axio Examiner Research Microscope...

Page 2: ...nted Offenders shall be liable for damages All rights reserved for any possible issue of a patent or for utility model registration All company or product designations used in this operating manual ma...

Page 3: ...ry microscope 28 3 1 5 Installing and removing Push Click modules 29 3 1 6 Installing a reflector insert 31 3 1 7 Installing an objective mount or objective nosepiece 31 3 1 8 Screwing in objectives 3...

Page 4: ...setting 56 3 4 1 Setting the eyepiece distance interpupillary distance at the binocular tube 56 3 4 2 Setting the viewing height 56 3 4 3 Compensating for eye deficiencies when using eyepiece reticule...

Page 5: ...ESHOOTING AND SERVICE 82 5 1 Equipment care 82 5 2 Equipment maintenance 82 5 2 1 Checks to be performed 82 5 2 2 Replacing fuses 83 5 3 Trouble shooting 84 5 4 Spares wearing parts and tools 87 5 5 S...

Page 6: ...ge on the stage carrier 34 Figure 3 15 Setting the ergonomic drive 34 Figure 3 16 Replacing the universal mounting frame 35 Figure 3 17 Attaching the stage carrier stage not shown in the drawing 36 Fi...

Page 7: ...aminer D1 with DIC equipment 63 Figure 4 6 Adjusting oblique illumination 65 Figure 4 7 Inserting a segmented stop slider 66 Figure 4 8 Components for reflected light fluorescence Axio Examiner A1 68...

Page 8: ...of Directive 98 79 EC and they are marked with the sign The present operating manual contains information and warnings which are to be observed by the operator The following notices and warning symbo...

Page 9: ...ffective any longer For restarting the equipment call the Zeiss customer service or the Carl Zeiss Microscopy Service The external power supply unit for 12 V DC 100 W for HAL 100 is designed for using...

Page 10: ...s not in use Before covering the equipment always check that all lighting devices have been switched off Clogged or covered venting slots may lead to heat buildup which may damage the equipment and ev...

Page 11: ...o warranty is granted for defects which are either due to natural wear and tear especially for wearing parts or to improper handling The equipment manufacturer shall not be liable for any damage cause...

Page 12: ...top or bottom part combination selected Legend for Figure 1 2 1 Microscope camera 2 Magnification changer 3 Tube 4 Upper microscope part 5 A 14 x 40 mm stop slider 6 A 14 x 40 mm stop slider or FL att...

Page 13: ...INTRODUCTION Axio Examiner List of interfaces Carl Zeiss M60 2 0003 e 05 2012 13 Figure 1 2 List of interfaces...

Page 14: ...ed stop slider 9 Pinion knob for mechanical stage positioning along the Y axis 10 Pinion knob for mechanical stage positioning along the X axis 11 Centering screw for condenser on both sides 12 Filter...

Page 15: ...INTRODUCTION Axio Examiner Controls and functional elements for Axio Examiner man Carl Zeiss M60 2 0003 e 05 2012 15 Figure 1 3 Controls and functional elements for Axio Examiner man...

Page 16: ...lider 10 Pinion knob for mechanical stage positioning along the Y axis 11 Pinion knob for mechanical stage positioning along the X axis 12 Centering screw for condenser on both sides 13 Filter wheel 6...

Page 17: ...INTRODUCTION Axio Examiner Controls and functional elements for Axio Examiner mot Carl Zeiss M60 2 0003 e 05 2012 17 Figure 1 4 Controls and functional elements for Axio Examiner mot...

Page 18: ...ted light and reflected light microscopes if equipped with a reflected light fluorescence system These microscopes are intended for medical applications to examine tissue samples obtained from the hum...

Page 19: ...EQUIPMENT DESCRIPTION Axio Examiner System overview Carl Zeiss M60 2 0003 e 05 2012 19 2 2 System overview...

Page 20: ...EQUIPMENT DESCRIPTION Carl Zeiss System overview Axio Examiner 20 M60 2 0003 e 05 2012...

Page 21: ...EQUIPMENT DESCRIPTION Axio Examiner System overview Carl Zeiss M60 2 0003 e 05 2012 21...

Page 22: ...EQUIPMENT DESCRIPTION Carl Zeiss System overview Axio Examiner 22 M60 2 0003 e 05 2012...

Page 23: ...726 mm x 470 mm Weight Axio Examiner A1 approx 19 kg Axio Examiner D1 approx 21 kg Axio Examiner Z1 incl control box approx 28 kg control box approx 5 5 kg Environmental conditions Transportation in...

Page 24: ...40 V 10 No line voltage adjustment is required Power frequency 50 to 60 Hz Power consumption for operation with HAL 100 220 VA HBO 100 W power supply unit Field of application indoor Safety class I De...

Page 25: ...scale interval Maximum stroke range 15 mm Height stop mechanically adjustable Condenser achromatic aplanatic 0 9 H DIC 5x for bright field dark field phase contrast 1 2 3 and DIC Manual objective exc...

Page 26: ...to read and understand the Equipment Safety Notices carefully before you install the microscope or start using it see page 8 3 1 Installing standard components 3 1 1 Unpacking and installing the micro...

Page 27: ...3 1 9 3 1 3 Attaching a binocular tube phototube Every binocular tube included in the system overview see section 2 2 may be attached to the Axio Examiner stand Proceed as follows Loosen the hexagon s...

Page 28: ...Br foc are intended for use together with eyepiece reticules The slight image displacement caused by the additional length of the glass path is taken into account on the diopter scale by the fact that...

Page 29: ...reflector turret is permanently installed to the Axio Examiner D1 Z1 microscope top parts To either install or remove the modules take off the cap and proceed at an angle from the front side 1 Install...

Page 30: ...ector insert reflector turret 4x or 6x or reflector slider 2x needs to be taken out of the stand top part 1 Installing a module Take the reflector insert Figure 3 7 1 out of the stand top part see sec...

Page 31: ...bjective nosepiece To attach the objective mount or objective nosepiece to the stand please proceed as follows For installation move to the lowest Z position possible using the pinion knob Figure 3 9...

Page 32: ...the WORK position Take the dust caps if any out of the corresponding openings at the objective mount or objective nosepiece Take the objective s Figure 3 11 3 out of their container and screw them int...

Page 33: ...r Hold the condenser carrier Figure 3 12 2 with one hand and unscrew the two hexagon socket screws Figure 3 12 1 Take off the condenser carrier and lay it down safely 3 1 10 Installing the condenser T...

Page 34: ...the drive length for X and Y axis positioning may be increased by a maximum of 15 mm by displacing the pinion knobs in axial direction Removing or installing additional 3 1 11 2 sleeves Both pinion k...

Page 35: ...Y pinion knob Figure 3 15 7 in position and turn the light colored knurled sleeve Figure 3 15 8 located above either to the right easy or to the left heavy until the desired easy motion is obtained Sl...

Page 36: ...17 2 slightly with your right hand Align the stage carrier correspondingly at the lower stop screw and tighten the clamping screw Figure 3 17 2 without using excessive pressure If the adapter is used...

Page 37: ...l guide Figure 3 17 1 at the right hand side Then remove the stage carrier from the stand by pulling it toward the front and lay it down on the table preventing it from overturning 3 1 13 HAL 100 halo...

Page 38: ...en set the adjusting screws Figure 3 19 4 and 5 to a value at which the lamp filaments of one image exactly fill out the gaps in the reflector image Figure 3 19 1 2 Fine adjustment Attach the microsco...

Page 39: ...ctor Push down the unlock key Figure 3 20 3 of the HAL 100 halogen lamp Figure 3 20 1 pull out the bulb holder Figure 3 20 2 completely and put it down separately Push down both spring levers Figure 3...

Page 40: ...separately Press the spring lever Figure 3 22 5 at the heat sink pull the heat sink Figure 3 22 1 upwards and off the inserted dummy or off the used burner Figure 3 22 2 and lay it down carefully Mak...

Page 41: ...ing shown at the hours run meter transformer Replace the burner when it has reached its nominal useful life of 100 hours Attaching the HBO 50 illuminator 3 1 14 2 Attach and align the HBO 50 illuminat...

Page 42: ...e in a centered location in parallel to one another within the adjusting circle in the observation window Figure 3 24 5 Electric arc and mirror image should not be superimposed one on top of the other...

Page 43: ...hanging transmission unless permanently installed Neutral density filters will not last in the long run Attaching the HBO 100 3 1 15 2 illuminator Remove the cap from the reflected light socket Figure...

Page 44: ...omewhat darker mirror image will appear in the adjusting aid s black glass window Turn the knurled knob Figure 3 28 4 to focus the brighter focal spot using the collector adjustment Turn the adjusting...

Page 45: ...rner mirror image by analogy to the focal spot image Figure 3 27 2 within the inscribed adjusting circle The two focal spots of the HBO 103 W 2 lamp should be located close to one another within the a...

Page 46: ...unit 3 2 1 Connecting the Axio Examiner mot In the Axio Examiner mot lower microscope part 430031 9010 000 focusing is insured exclusively by the motor driven Z axis positioning of the objective moun...

Page 47: ...ner control unit 3 2 2 Connecting and switching the HAL 100 halogen lamp on or off The HAL 100 halogen lamp is powered and switched either on or off by the external power supply unit The external powe...

Page 48: ...of the HBO 100 W transformer and secure it by a retaining ring Connect the HBO 100 W transformer s AC power socket Figure 3 31 3 to a mains outlet Use the power switch Figure 3 31 1 to switch the HBO...

Page 49: ...to the assembly opening located at the front side and use it to lift off the cover Hold the stand top part Figure 3 32 4 in position screw out the six hexagon socket head screws Figure 3 32 2 and remo...

Page 50: ...33 3 Put on the cap with the opening Figure 3 33 2 Slide in the slidable adjusting aid socket 3 3 3 Installing an adapter for 45 mm objectives Only for Axio Examiner D1 Z1 Use the shift knob Figure 3...

Page 51: ...ter set in the FL P C reflector module The filter sets for the FL P C reflector module may be combined and installed on an individual basis by the customer Corresponding filter sets or completely asse...

Page 52: ...Figure 3 38 2 and spring frame Figure 3 38 3 out of the lower half module Remove the old beam splitter and position the new beam splitter with the reflecting side pointing down onto the spring frame F...

Page 53: ...the guide of the mounting frame Attach condenser and stage carrier again 3 3 8 Replacing the DIC prism in the condenser To take out the cover plate turn the condenser cover plate Figure 3 40 4 using t...

Page 54: ...d Figure 3 41 2 up and out of the corresponding position of the filter wheel Figure 3 41 3 using a pair of tweezers Insert a new filter into the filter wheel Figure 3 41 3 A filter position is always...

Page 55: ...rim must fit in the slot at the condenser Run the discharge hose Figure 3 42 2 to a corresponding vessel dish etc Put on the stage carrier see section 3 1 12 3 3 11 Attaching a condenser carrier with...

Page 56: ...iece reticules The correct use of an eyepiece reticule requires two adjustable eyepieces e g PL 10x 23 Br foc for compensating different eye deficiencies of a viewer Use the focusable eyepiece lens of...

Page 57: ...lock in resistance is set Insert the closing plug again Figure 3 46 1 Put on the binocular tube see section 3 1 3 3 4 5 Setting the actuating rod Only for phototubes 425521 9020 000 and 425521 9000 00...

Page 58: ...oscopy procedures since it allows the quick and easy viewing of high contrast or stained specimens e g blood films In addition to so called direct beams indirect ray beams i e beams diffracted and sca...

Page 59: ...ut of position by the condenser Switch the revolving disk of the condenser where existing using the knurled ring Figure 4 2 4 to the H position for the bright field Swing the 10x objective in position...

Page 60: ...nge changes both the visual field size and the objective aperture This requires a new setting of bright field and aperture stop in order to achieve optimum results If objectives 10x are used the apert...

Page 61: ...ermediate image while different path difference are transposed into different grey values intensities A compensator lambda plate converts the grey values into colors subsequently 2 Equipment Objective...

Page 62: ...locks exactly into position Axio Examiner D1 Z1 with W Plan Apochromat 20x 1 0 objective 75 mm parfocal length Insert DIC slider S NARMONT 75 W PA 426967 9000 000 or 426967 9070 000 Figure 4 4 1 into...

Page 63: ...e 10x objective or the overview objective used Figure 4 5 4 is in working position The condenser turret has been swiveled into the bright field or into an unequipped position Swing the S NARMONT polar...

Page 64: ...revolving disk The condenser used must not be equipped with DIC prisms which are installed in combination with polarizing filters Switch on the S NARMONT polarizer Figure 4 5 2 polarizer and 4 plate i...

Page 65: ...edure HAL 100 halogen lamp for transmitted light illumination The following condensers may be used for oblique illumination Condenser 0 8 424226 9000 000 Condenser 0 9 424226 9010 000 Condenser 1 2 42...

Page 66: ...rticular for highly scattering tissue sections 2 Equipment The equipment of every microscope allows the performance of the oblique illumination procedure All condensers available for delivery may be u...

Page 67: ...litter Finally the beams pass a barrier filter long pass band pass filter which only allows the passage of the long wave radiation emitted by the specimen Excitation filter and barrier filter require...

Page 68: ...itation at the reflector turret Figure 4 8 6 Enable the optical path in the reflected light illuminator when using the reflected light filter drawer Figure 4 8 2 If required set the illumination inten...

Page 69: ...inocular phototube Actuating rod Actuating rod pushed in Visual examination through the eyepieces Actuating rod pulled out Camera output enabled Shift knob Turn the shift knob Figure 1 3 3 to toggle b...

Page 70: ...see Figure 4 9 Navigation bar 4 3 1 1 The navigation bar is located at the left hand screen side and may be used to open all pages The keys available depend on the current page But the following keys...

Page 71: ...keys work like switches 3 Operator control This area contains control elements which depend on the button selected either in the navigation bar or in the tab 4 Popup windows Popup windows will appear...

Page 72: ...Info Extras Components XY Z Soft keys Position Focus Mode Buttons Left Buttons Right Stand type Language Ethernet Misc Reflector Cond Focus Navigation bar 2nd level Reflector Misc Firmware Figure 4 11...

Page 73: ...l motors or sensors of the microscope either on or off Motors and sensors will be grounded earthed potential free when switched off and the blue indicator light Figure 1 4 19 will go off at the lower...

Page 74: ...The two Set zero buttons work as follows man Set the zero point manually i e the current position is defined as the zero point and the display is set to zero auto Set the zero point automatically i e...

Page 75: ...te positions see 2 Save position above 4 3 6 Settings Press the Settings button in the navigation bar on the Home page to go to the Settings page From the Settings page you can go to the Components Us...

Page 76: ...b to set the focusing gear speed compensate objective parfocality or switch automatic parfocality compensation either on or off 3 Misc ellaneous This tab enables the operator to configure additional o...

Page 77: ...12345 2 Buttons left To modify the button assignments the operator needs to type in an administrator password Otherwise the operator may only view the button assignments without being able to customi...

Page 78: ...6 4 The Settings About page only contains the Firmware tab This tab indicates the firmware version to the operator 4 3 7 Display Press the Display button in the navigation bar on the Home page to ope...

Page 79: ...ay be written on the adhesive labels supplied The labels may be glued to the fields provided 4 4 2 Axio Examiner Z1 upper microscope part with sideport The Axio Examiner Z1 upper microscope part with...

Page 80: ...To enable the click stop again rotate the adjusting screw Figure 4 21 3 by 90 in the opposite direction 2 Adjusting the cameras to one another If two cameras are used they need to be adjusted to one...

Page 81: ...s M60 2 0003 e 05 2012 81 Unscrew the knurled screw Figure 4 22 1 at the camera port on the rear side To adjust the camera in X and Y direction turn the two adjusting screws width A F 1 5 Figure 4 22...

Page 82: ...the center to the rim using a slightly moistened swab Remove dust from optical surfaces using a natural hair brush or blow it off using a rubber ball air blower Please observe the following when you...

Page 83: ...inlet and contains two fuses of the following types T 5 0 A H 5x20mm Examiner control and 12 V DC 100 W external power supply unit or T 2 0 H A HBO 100 transformer or T 4 A mbq52ac z 100 V 127 V trans...

Page 84: ...correctly centering opening see page 58 and the following Filter not placed correctly into the filter holder Place filter correctly into the filter holder Low resolution and bad image contrast Apertur...

Page 85: ...distance not set correctly at the binocular tube Adjust the eyepiece distance correctly see page 56 Focusable eyepieces are not adjusted correctly Adjust the focusable eyepieces to the eye deficiencie...

Page 86: ...halogen lamp is flickering Its luminosity is not stable The 12 V 100 W halogen lamp is at the end of its mean service life Replace the 12 V 100 W halogen lamp see page 39 AC power cord not installed...

Page 87: ...screwdriver width A F 2 5 Ball head screwdriver width A F 1 5 For setting oblique illumination at the condenser Eyepiece eyecup Recommendable for reflected light suppression during low luminosity proc...

Page 88: ...service or by especially authorized expert personnel To ensure the optimum setting and trouble free operation of your microscope for a long period of time we recommend to sign a service or maintenance...

Page 89: ...ards Institution DL Transmitted light DX Coding system for storing electronically readable information e g film speed EG European Community EN Euronorm EMC Electromagnetic compatibility ENG Electronic...

Page 90: ...across flats T Slow blow a fuse type TV Television T2 Adapter Standardized adapter for miniature cameras UL Underwriter Laboratories UV Ultraviolet VDE Verband Deutscher Elektrotechniker Association...

Page 91: ...ce 82 Click stop 80 Coarse motion 14 16 Colibri 45 Condenser 14 16 33 53 55 59 Height adjustment 14 16 Condenser carrier 33 motor operated 55 Conversion 49 D DIC 61 DIC condenser module 53 DIC slider...

Page 92: ...45 I Illumination and contrast procedures 58 Illuminator HBO 100 44 Illuminator HBO 50 40 Infrared 14 16 Installing 26 IR 60 K Key ring 16 K HLER 58 L Light sources 24 Line voltage 24 25 Lock in resi...

Page 93: ...minator 68 Reflector insert 31 Reflector module 52 Reflector turret 16 25 57 79 Reflector turret mot 46 S Segmented stop 14 16 Segmented stop slider 14 16 S NARMONT polarizer 53 63 Service 88 Shift kn...

Page 94: ...APPENDIX Carl Zeiss Subject index Axio Examiner 94 M60 2 0003 e 05 2012 V Viewing height 56 VIS 14 16 60 W Warning signs 11 Warranty instructions 11 Wearing parts 87 Weight 23...

Page 95: ...DIX Axio Examiner Industrial property rights Carl Zeiss M60 2 0003 e 05 2012 95 6 3 Industrial property rights The equipment equipment parts or procedures described in this manual are protected by pat...

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