OPERATION
ZEISS
Brightfield transmitted light microscopy - quick guide
Axio Observer
100
431004-7244-001
12/2016
5.4
Brightfield transmitted light microscopy - quick guide
Before starting to work with the microscope, first read the sections 2, 3 and 4.
•
Prepare the microscope and switch it on in accordance with section 4 (Fig. 83/
1
).
•
Select the objective with the lowest magnification (e.g. objective 10x - yellow ring) on the nosepiece
(Fig. 83/
13
); ensure that it clicks into position correctly. Set factor 1x on the selector wheel (Fig. 83/
16
)
of the Optovar turret.
•
Open the luminous-field diaphragm completely by turning the luminous-field diaphragm control
(Fig. 83/
7
) on the carrier for transmitted-light illumination to the left.
•
Open the aperture diaphragm completely by rotating the selector wheel on the condenser forwards as
far as it will go.
•
Turn the condenser turret adjustment ring (Fig. 83/
3
) to move the condenser turret to the
H
position
for brightfield (if H is not available, to the
DIC
position).
•
Turn the adjustment ring to move the reflector turret (if available) to a position which contains no filter
combination. Ensure that it clicks into position correctly.
•
If necessary remove the analyzer slider from the slot (Fig. 83/
12
) or move to the open position. Ensure
that it clicks into position correctly.
•
Turn the sideport selector wheel (Fig. 83/
2
) to the 100 % vis (visual) position.
•
Set the beam splitting ratio to 100 % vis (Fig. 83/
20
) on the binocular (photo)tube. Remove the
Bertrand lens from the optical path (where used). To do this, move the rotary / sliding button
(Fig. 83/
21
) to the 100 % vis position.
•
Swivel the 3-position filter changer (Fig. 83/
5
) out of the optical path.
•
Place a high-contrast specimen on the microscope stage (Fig. 83/
10
).
•
Match the eyepiece distance (interpupillary distance) to the user's individual interpupillary distance: For
this purpose, pull apart or push together the binocular component (Fig. 83/
22
) of the tube.
•
Set the ametropia correction zero point using the adjustment ring (Fig. 83/
24
) of the eyepieces
23
):
without eyepiece reticles:
set to the white point,
with eyepiece reticles:
set to the red point.
•
To correct for ametropia, bring the selected detail of the specimen into optimum focus using the
relevant eyepiece adjustment ring.
•
Use the coarse / fine focus drive (Fig. 83/
17
) to focus on the selected detail of the specimen. If no light is
visible in the eyepieces, check that light is being emitted from the housing of the halogen illuminator. If
no light is being emitted, switch on the halogen illuminator by pressing the TL button (Fig. 83/
18
).
•
Set the light intensity to a comfortable level by using the illumination control wheel (Fig. 83/
19
).
•
Close the luminous-field diaphragm (Fig. 83/
7
)
until it is visible in the field of view (not
necessarily in focus) (Fig. 82/
A
).
•
Bring the edge of the luminous-field diaphragm
into focus (Fig. 82/
B
) by adjusting the height of
the condenser (Fig. 83/
8
).
•
Center (Fig. 82/
C
) the luminous-field diaphragm
using the centering screws (Fig. 83/
4
) and open
until the edge of the diaphragm just disappears
from the field of view (Fig. 82/
D
).
•
To set the aperture diaphragm, remove one eyepiece from the eyepiece tube and adjust the aperture
diaphragm to approximately 2/3 the diameter of the exit pupil of the eyepiece (Fig. 82/
E
).
The settings required for optimum contrast depend on the specimen.
Fig. 82
Diaphragm settings in transmitted
light brightfield acc. to KÖHLER
Summary of Contents for Axio Observer Series
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Page 192: ......
