OPERATION
Axio Observer
Illumination and contrast techniques
ZEISS
12/2016
431004-7244-001
155
5.12.4.3
Setting up PlasDIC
The microscope has been started properly as described in section 4.
•
Place a specimen.
•
Set the specimen for transmitted light brightfield.
•
Select a PlasDIC objective.
•
Insert the PlasDIC slider (Fig. 153/
3
) into the DIC slot of the objectives to be used and additionally
swivel or slide an analyzer, e.g. the analyzer module Pol ACR P&C of the reflector turret or the
analyzer slider, fixed, into the optical path.
or
•
Insert the 3-position contrast slider (Fig. 153/
1
) with fitted PlasDIC module (Fig. 153/
2
) into the slot
below the nosepiece.
If the PlasDIC module has not yet been fitted into the 3-position contrast slider:
−
Loosen the screws (Fig. 153/
1.2
) on the cover (Fig. 153/
1.1
) of the contrast slider and
remove the cover.
−
Insert the PlasDIC module (Fig. 153/
2
) into the contrast slider and fix with the screws
1.2
).
When using the PlasDIC module in the 3-position contrast slider an analyzer is not required.
Fig. 153
Setting the PlasDIC contrast
•
Open the condenser aperture diaphragm fully.
•
Swivel in the slit aperture 3.5 mm PlasDIC (or 5 mm, depending on objective and condenser) on the
condenser including the modulator disk.
•
When switching from bright field to PlasDIC, the brightness should be increased.
•
Adjust the contrast using the knurled screw on the PlasDIC slider or PlasDIC module.
Structures can be visualized in relief or pseudo-dark field.
Relief offers the best contrast.
Summary of Contents for Axio Observer Series
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