OPERATION
ZEISS
Illumination and contrast techniques
Axio Observer
160
431004-7244-001
12/2016
•
Switch on the HXP 120 V or HBO 100 fluorescence illuminator and leave to warm up to operating
temperature for approx. 15 minutes.
•
On the reflector turret (Fig. 154/
7
) or the virtual reflector turret (if dual filter wheel mot. is available),
select the desired fluorescence filter combination (depending on the desired kind of excitation) and
switch on.
•
Open the fluorescence shutter by pressing the RL button.
For reflected light fluorescence, two iris diaphragm sliders are used as aperture and luminous-field
diaphragms. However, since the aperture diaphragm is not visible during the reflected light fluorescence
procedure, the aperture diaphragm slider must first be centered in the luminous-field diaphragm slot and
then inserted in the aperture diaphragm slot.
The aperture diaphragm and luminous-field diaphragm are centered in the same way:
•
Insert an iris diaphragm slider into the luminous-field diaphragm F slot (Fig. 154/
3
) until it clicks into
place.
•
Using the lever (Fig. 156/
4
), close the diaphragm until it becomes visible in the field of view.
•
Center the diaphragm using the two adjusting screws (Fig. 156/
2
,
3
) on the slider (using a 3 mm ball-
headed screwdriver). Finally, open the diaphragm until the entire field is clear.
•
Insert the centered slider into the slot A (Fig. 154/
2
) for the aperture diaphragm until it clicks into
place.
•
Insert a further iris diaphragm slider into the luminous-field diaphragm slot.
•
Close the luminous-field diaphragm until it becomes visible in the field of view.
•
Center the luminous-field diaphragm F to the edge of the field of view using the two centering
screws.
•
Either open the luminous-field diaphragm until it just disappears from the edge of the field of view or,
if there is a risk of bleaching the specimen, close it until it is visible in the field of view.
•
Finally, refocus on the specimen and optimize the position of the fluorescence illuminator (HBO 100,
HBO 50). For this purpose, use the knurled knob on the fluorescence illuminator to set the collector so
that the field of view is illuminated as evenly as possible when using the short-wave excitation
reflector module. When long-wave excitation modules are used, no correction of the collector position
is required.
Summary of Contents for Axio Observer Series
Page 1: ...Axio Observer Inverted microscope Operating manual...
Page 192: ......
