OPERATION
ZEISS
Illumination and contrast techniques
Axio Observer
164
431004-7244-001
12/2016
5.12.9
Setting up reflected light DIC and reflected light C-DIC
(1)
Application
The reflected light DIC and reflected light C-DIC
technique (DIC = Differential Interference Contrast,
C-DIC = Differential Interference Contrast in
Circularly polarized light) produces high-contrast
images of phase specimens, i.e. those specimens
which only change the phase of the light in
contrast to amplitude specimens.
(2)
Instrument equipment
−
Axio Observer materials with attached microLED
or adjusted HAL 100 illuminator
−
Objectives: EC Epiplan-Neofluar or Epiplan with
additional designation "DIC" or "Pol".
−
DIC slider matching the respective objective (its
magnification and aperture are engraved on the
top side of the slider)or C-DIC slider (in
combination with reflector module CDIC P&C).
−
DIC/Pol P&C reflector module or DIC/Pol Red I
P&C in reflector turret or
C-DIC/TIC P&C reflector module (in combination
with C-DIC slider 6x20).
(3)
Reflected light DIC
•
Prepare the microscope as described in section 5.12.5 for reflected light brightfield. Open the
luminous-field diaphragm until the edge just disappear from the field of view to avoid reflections.
•
Swivel in the reflector module DIC/Pol P&C on the reflector turret into the beam path. To produce
color contrasts, use reflector module DIC/Pol Red I P&C, which is of advantage in case of large
retardation (> 1
λ
).
•
Rotate the nosepiece to swivel in the objective position with DIC slider slot.
•
Insert the DIC slider (Fig. 158/
2
) into the slot on the nosepiece.
•
Place the specimen on the stage, bring it into focus until the specimen structure of interest appears at
maximum contrast.
•
To optimize the contrast, turn the knurled screw on the DIC slider.
1
C-DIC or TIC slider , 6x20
2
DIC slider
Fig. 158
DIC / C-DIC reflected light
components on
Axio observer materials
Summary of Contents for Axio Observer Series
Page 1: ...Axio Observer Inverted microscope Operating manual...
Page 192: ......
