OPERATION
Axio Observer
Illumination and contrast techniques
ZEISS
12/2016
431004-7244-001
165
(4)
Reflected light C-DIC
•
Prepare the microscope for reflected light brightfield.
•
Rotate reflector turret to swivel C-DIC/TIC P&C reflector module (Fig. 154/
7
) into the beam path.
•
Insert the C-DIC slider 6x20 (Fig. 158/
1
) into the slot for C-DIC slider.
•
If necessary, refocus and rotate the selector wheel on the C-DIC slider 6x20 (Fig. 158/
1
) until the
structure of interest is visible at maximum contrast.
•
Additional contrasting effects can be produced by adjusting the selector wheel on the C-DIC slider.
(5)
Additional note
Differential interference contrast or differential interference contrast with circularly polarized light is
created by a (pseudo) relief in the specimen. Therefore, it depends on whether linear structures are
oriented in the "light-shadow" direction (very faint contrast) or vertical to this direction (maximum
contrast).
If you use the C-DIC slider 6x20, you can turn the selector wheel of the C-DIC slider to align the
structures vertically to the "light-shadow" direction and thus achieve maximum contrast.
Summary of Contents for Axio Observer Series
Page 1: ...Axio Observer Inverted microscope Operating manual...
Page 192: ......
