Zeiss Axio Observer Series, Operating Manual

Page 1: ...Axio Observer Inverted microscope Operating manual...

Page 2: ...mmunication of its contents are not permitted Persons in contravention of this copyright shall be liable for damages All rights reserved in the event of granting of patents or registration as a utilit...

Page 3: ...binocular photo tube 38 4 4 1 Inserting the eyepieces and auxiliary microscope 38 4 4 2 Inserting the eyepiece reticle 39 4 5 Fitting the carrier for transmitted light illumination 40 4 6 Fitting the...

Page 4: ...mercury vapor short arc bulb 68 4 23 2 Fitting the HBO 100 illuminator 68 4 23 3 Adjusting the HBO 100 illuminator 69 4 24 HXP 120 V illuminator 69 4 25 Colibri 2 and Colibri 7 illumination system 69...

Page 5: ...smitted light PlasDIC contrast 153 5 12 5 Setting up reflected light bright field 156 5 12 6 Setting up reflected light fluorescence contrast 159 5 12 7 Setting up reflected light polarization 161 5 1...

Page 6: ...ials for material applications MAT meet the requirements of the European low voltage directive 2014 35 EU and the EMC directive 2014 30 EU and are marked with the mark These Safety Instructions contai...

Page 7: ...ct conforms with all valid European directives applicable to it ATTENTION This symbol indicates a potential hazard to the instrument or system ATTENTION Read the Operating Manual ATTENTION Stand by Th...

Page 8: ...7344 001 The Axio Observer may not be put into operation if the dust cover is still on Before putting on the dust cover always check that the device is switched off and has cooled down otherwise this...

Page 9: ...LED risk category 2 under DIN EN 62471 2009 Do not look directly into the illumination light The desktop power supply units which are available as a microscopy accessory permit mains voltages in the 1...

Page 10: ...instrument including the use of individual modules or components Modifications and repairs to this instrument and any devices operated in combination with the Axio Observer are to be carried out by ou...

Page 11: ...evice from the mains power supply before gaining access to the interior of the device or changing the fuse Only use fuses for the given rated current which comply with the specifications on the fuse h...

Page 12: ...ble design of the workplace environment 2 2 Warranty information The manufacturer guarantees that the device is free of material or manufacturing defects when delivered Any defects must be reported im...

Page 13: ...Observer Warning and information labels on the device ZEISS 12 2016 431004 7244 001 13 2 3 Warning and information labels on the device Fig 1 Warning labels on the microLED attachment lamp Fig 2 Labe...

Page 14: ...zed version including motorized Z drive Axio Observer microscopes 3 5 7 Fig 3 are inverted light microscopes for universal use They are used primarily for examining cell and tissue cultures and sedime...

Page 15: ...al use They are used in all areas of research based and industrial microscopy Inverted microscopes permit the unrestricted use of conventional samples as a result of the highly accommodating sample co...

Page 16: ...rials finding the causes of component failures in process inspection of cast worked or machined components and semi finished products Various investigations of composite materials and material compoun...

Page 17: ...larization contrast Brightfield Polarization contrast Darkfield Differential Interference Contrast DIC Differential Interference Contrast in circularly polarized light C DIC Total Interference Contras...

Page 18: ...osition Optovar turret Removable aperture diaphragm and luminous field diaphragm sliders for reflected light Fluorescence shutter internal standard shutter or external high speed shutter HAL 100 HBO 1...

Page 19: ...n CAN hub O O O Light Manager Contrast Manager Control ring right left Z focus drive manual 2 mm 0 2 mm motorized stepper motor drive z step size 10 nm Adjustable vertical stop for Z drive focus stop...

Page 20: ...R O O 60N L R 3 switching positions 100 vis 0 LR 0 vis 100 L 20 vis 80 R O O 60N R L 100 3 switching positions 100 vis 0 LR 0 vis 100 L 0 vis 100 R O O 60N L 80 R 100 3 switching positions 100 vis 0...

Page 21: ...position coded O O 6 position motorized O O 6 position motorized ACR O O Switching mirror mot CAN motorized O O Filter wheels Dual filter wheel mot for beam splitting and emission CAN O Filter wheel e...

Page 22: ...Light Manager Contrast Manager Control ring right left Z focus drive manual 2 mm 0 2 mm motorized stepper motor drive z step size 10 nm Adjustable vertical stop for Z drive focus stop manual Automatic...

Page 23: ...vis 100 R O O 60N L 80 R 100 3 switching positions 100 vis 0 LR 20 vis 80 L 0 vis 100 R O O Path deflection to the tube VIS only O O Beam path switching for VIS front port base port manual O motorize...

Page 24: ...orized O O 6 position motorized ACR O O Filter wheel excitation 8 pos mot for filters d 25 mm CAN motorized O O Switching mirror mot CAN motorized O O ApoTome ApoTome 2 O O included in stand 1 include...

Page 25: ...SCRIPTION OF THE DEVICE Axio Observer System overview Axio Observer Bio Med ZEISS 12 2016 431004 7244 001 25 3 4 System overview Axio Observer Bio Med Fig 5 System overview Axio Observer Bio Med Sheet...

Page 26: ...DESCRIPTION OF THE DEVICE ZEISS System overview Axio Observer Bio Med Axio Observer 26 431004 7244 001 12 2016 Fig 6 System overview Axio Observer Bio Med Sheet 2...

Page 27: ...DESCRIPTION OF THE DEVICE Axio Observer System overview Axio Observer Bio Med ZEISS 12 2016 431004 7244 001 27 Fig 7 System overview Axio Observer Bio Med Sheet 3...

Page 28: ...DESCRIPTION OF THE DEVICE ZEISS System overview Axio Observer Bio Med Axio Observer 28 431004 7244 001 12 2016 Fig 8 System overview Axio Observer Bio Med Sheet 4...

Page 29: ...ON OF THE DEVICE Axio Observer System overview Axio Observer materials Mat ZEISS 12 2016 431004 7244 001 29 3 5 System overview Axio Observer materials Mat Fig 9 System overview Axio Observer material...

Page 30: ...DESCRIPTION OF THE DEVICE ZEISS System overview Axio Observer materials Mat Axio Observer 30 431004 7244 001 12 2016 Fig 10 System overview Axio Observer materials Sheet 2...

Page 31: ...DESCRIPTION OF THE DEVICE Axio Observer System overview Axio Observer materials Mat ZEISS 12 2016 431004 7244 001 31 Fig 11 System overview Axio Observer materials Sheet 3...

Page 32: ...DESCRIPTION OF THE DEVICE ZEISS System overview Axio Observer materials Mat Axio Observer 32 431004 7244 001 12 2016 Fig 12 System overview Axio Observer materials Sheet 4...

Page 33: ...sible air humidity without condensation max 75 at 40 C Transport in packaging Permissible ambient temperature 40 C to 70 C Permissible air humidity without condensation max 75 at 35 C Operation Permis...

Page 34: ...e in accordance with EN 55011 Class B Noise immunity in accordance with DIN EN 61326 1 Line voltage 100 to 127 V and 200 V to 240 VAC 10 Axio Observer 3 3 materials 5 5 materials Line voltage of exter...

Page 35: ...al data Stand with stage focusing with coarse focusing drive approx 2 mm rotation and fine focusing drive approx 1 10 coarse fine focus transmission ratio total travel approx 10 mm 13 mm also possible...

Page 36: ...o be ensured that the floor on which the support table stands is subject to the least possible vibration Only operate the device on a hard non flammable surface The parameters described in sections Am...

Page 37: ...inator and a number of small components such as filter sliders and diaphragm sliders DIC slider dust cover and tools Further optional accessories are supplied in a separate case Remove all components...

Page 38: ...ich is to be attached Insert the binocular tube Fig 14 1 with the dovetail ring into the tube mount Fig 14 3 on the stand align it with the stand and tighten the Allen screw Fig 14 2 using the ball he...

Page 39: ...ed eyepiece reticle If you wish to insert an eyepiece reticle into a mount you should ensure that the labeling on the eyepiece is still visible in the correct position after the eyepiece has been rein...

Page 40: ...in position against the rear of the stand and screw into place using the four Allen screws provided Fig 17 2 and the 4 mm Allen key Insert the LCD display connector Axio Observer 5 and 5 materials onl...

Page 41: ...recesses are positioned on top of the two screws Insert the LCD display plug into the socket Fig 18 3 for the display carrier for transmitted light illumination on the rear of the 5 materials stand 4...

Page 42: ...using the DMR large lens hood 431716 0170 000 When attaching the lens hoods ensure that the upper edge does not form a drip tray Unused nosepiece openings should be sealed using the caps supplied Atta...

Page 43: ...r mounting frame K 432016 9902 000 4 mm Mechanical stage 130x85 R L with short coaxial drive for mounting frame K 432047 9902 000 4 mm Scanning stage 130x100 PIEZO 432027 9001 000 4 mm Scanning stage...

Page 44: ...ks are included with each stand and stage If using flat mounting frames which are flush to the stage e g 451344 and 160x110 stage pinhole apertures e g 451345 9902 do not use spacer disks on mechanica...

Page 45: ...34 9001 000 Risk of collision Remove the front handle prior to using the microscope stand with a scanning stage Because of the extensive travel range of scanning stage 130x100 the stage frame may coll...

Page 46: ...tage and remove the covers Fig 24 2 Then loosen the stop screws Fig 24 3 move the stop as necessary and retighten the screws Then retighten the screws of the covers Scanning stage 130 x 85 mot CAN is...

Page 47: ...ht illumination Fig 26 4 if fitted can be tilted backwards Place the scanning stage 130x100 STEP Fig 26 1 on the three contact points Fig 26 5 of the stand and fix it in position using three Allen scr...

Page 48: ...be tilted backwards Place scanning stage 130x100 MAT CAN Fig 27 1 on the three contact points Fig 27 5 of the stand and fix it in position using three Allen screws two at the front Fig 27 3 one at th...

Page 49: ...Fig 28 3 onto the rear attachment point Place the specimen stage Fig 28 1 onto the stand and screw to the rear attachment point from above using the longer Allen screw Fig 28 2 Ensure that the screw p...

Page 50: ...nosepiece must be lowered fully using the focus drive before changing objectives as otherwise the objective may collide with the heated stage 4 9 7 Fitting the gliding stage Z Use three spacer disks...

Page 51: ...groove on the condenser socket Fix the condenser in place using the clamping screw Fig 31 2 In the case of motorized condensers connect the cable to the outlet socket on the mount left ATTENTION When...

Page 52: ...cing upwards Ensure that the locating pin on the condenser is at the front and engages precisely with the guide groove on the condenser carrier Fix the condenser mount in place using the clamping scre...

Page 53: ...n position using the knurled ring Unscrew the retaining ring Fig 33 2 using the mounting plate from the tool set Turn the condenser upside down and allow the DIC prism Fig 33 3 to slide out onto a sof...

Page 54: ...event the emission of refracted light Rotate the locking lever Fig 34 2 downwards and open the cover Fig 34 1 Insert the loaded or unloaded reflector turret Fig 34 3 into the opening beneath the nosep...

Page 55: ...n see position marking Fig 35 6 starting with position 1 emission filter is at the bottom To insert the reflector modules insert the retaining elements Fig 35 5 on the left and right of the module int...

Page 56: ...cure both filters with the retaining rings Fig 36 1 Barrier filter and exciter filter may bear a designation and an arrow on their circumference The arrow indicates the direction in which the filter m...

Page 57: ...ation and imaging beam path 4 11 4 Changing the beam splitter in reflector module FL P C Fitting the filters and the beam splitter requires utmost care to prevent damage to and contamination of the op...

Page 58: ...ether into the lower half of the module Ensure that the catch on the side of the spring loaded frame is positioned in the recess in the lower half of the module The reflective coated side Fig 40 3 of...

Page 59: ...isplay and docking station If the TFT display is fitted to the 7 7 materials stand it must first be removed from the stand After this cover the screw holes and the plug contact opening on the stand wi...

Page 60: ...output 2 4 Power socket 5 CAN connecting socket 6 USB port 4 15 Connectors on the rear of the Axio Observer 5 5 materials Key to Fig 44 1 Connector for transmitted light LED halogen illuminator outpu...

Page 61: ...1 Connector for closed loop sensor Z drive Axio Observer 7 only 2 Socket for power supply unit VP232 2 3 Connecting socket for transmitted light LED halogen illuminator 4 CAN connecting sockets 5 not...

Page 62: ...Definite Focus 2 module with Focus Controller 2 to stand 2 Connector of stage attachment Z PIEZO WSB 500 with controller to stand 3 Connector of auto immersion module to stand 4 Connector of desktop...

Page 63: ...and off Axio Observer 3 3 materials Switch the microscope on and off using the power switch on the left of the stand Fig 48 1 Axio Observer 5 5 materials Switch the microscope on and off using the sta...

Page 64: ...e microLED illuminator for transmitted and reflected light The microLED illuminator can be used for both transmitted light and reflected light Do not look directly into the LED light Always make sure...

Page 65: ...ee section 4 22 3 Remove the protective cap from the reflected light or transmitted light socket Insert the dovetail ring of the lamp housing Fig 50 7 into the corresponding socket Fig 50 8 or Fig 50...

Page 66: ...d filament image Fig 51 1 2 Fine adjustment If necessary loosen the clamping screw Fig 52 1 and remove the HAL 100 from the transmitted light illumination mount Manually unscrew the diffusion disk Fig...

Page 67: ...lected light or Fig 50 5 for transmitted light Allow to cool for about 15 min Press the release button Fig 53 3 on the HAL 100 illuminator Fig 53 1 downwards and remove the bulb holder Fig 53 2 comple...

Page 68: ...crete manual or motorized should be fitted into reflected light illuminator FL to change the transmission 4 23 2 Fitting the HBO 100 illuminator Remove the cover from the reflected light socket Fig 54...

Page 69: ...e eyes during observation of the bright focal spot Unscrew an objective and check that the light source image has free access to the specimen plane on the specimen stage using a piece of paper Focus t...

Page 70: ...components on the stand Loosen the four screws Fig 56 3 from the plastic cover Fig 56 4 and remove the cover from the stand by pulling to the right Mount the mounting adapter Fig 56 2 to the stand an...

Page 71: ...g functions of the six stand versions are described in sections 5 1 and 5 2 The Axio Observer 5 stand is equipped with an LCD on the transmitted light carrier and the Axio Observer 5 materials stand w...

Page 72: ...ing and function controls overview Axio Observer 72 431004 7244 001 12 2016 5 1 Operating and function controls overview 5 1 1 Axio Observer 3 manual with coded nosepiece Fig 57 Axio Observer 3 manual...

Page 73: ...for iris diaphragm slider as reflected light aperture diaphragm or FL attenuator see page 88 15 Slot for 3 position filter slider d 25 mm see page 88 16 HBO 100 illuminator 17 LM Set button see page 9...

Page 74: ...OPERATION ZEISS Operating and function controls overview Axio Observer 74 431004 7244 001 12 2016 5 1 2 Axio Observer 5 coded semi motorized Fig 58 Axio Observer 5 coded semi motorized...

Page 75: ...diaphragm slider as reflected light aperture diaphragm manual or motorized or FL attenuator manual or motorized see page 88 18 Slot for 3 position filter slider d 25 mm see page 88 19 Colibri 7 illumi...

Page 76: ...OPERATION ZEISS Operating and function controls overview Axio Observer 76 431004 7244 001 12 2016 5 1 3 Axio Observer 7 motorized Fig 59 Axio Observer 7 motorized...

Page 77: ...r FL attenuator manual or motorized see page 88 15 Slot for 3 position filter slider d 25 mm see page 88 16 Colibri 7 illumination system 17 LM Set button see page 92 18 Drive knobs for controlling XY...

Page 78: ...ZEISS Operating and function controls overview Axio Observer 78 431004 7244 001 12 2016 5 1 4 Axio Observer 3 materials manual with coded nosepiece Fig 60 Axio Observer 3 materials manual with coded...

Page 79: ...for aperture diaphragm slider MAT or FL attenuator see page 89 9 Slot for 3 position filter slider d 25 mm see page 88 10 Light manager set button see page 92 11 Drive knobs for controlling XY positi...

Page 80: ...ERATION ZEISS Operating and function controls overview Axio Observer 80 431004 7244 001 12 2016 5 1 5 Axio Observer 5 materials coded semi motorized Fig 61 Axio Observer 5 materials coded semi motoriz...

Page 81: ...re diaphragm slider MAT or FL attenuator manual or motorized see page 89 12 Slot for 3 position filter slider d 25 mm see page 88 13 LM Set button see page 92 14 Drive knobs for controlling XY positio...

Page 82: ...OPERATION ZEISS Operating and function controls overview Axio Observer 82 431004 7244 001 12 2016 5 1 6 Axio Observer 7 materials motorized Fig 62 Axio Observer 7 materials motorized...

Page 83: ...d 25 mm see page 88 10 LM Set button see page 92 11 Drive knobs for controlling XY positioning of the mechanical stage see page 89 12 Right sideport 13 6 position nosepiece HD DIC M27 mot ACR motorize...

Page 84: ...rials stand always flat already included in stand Light path selector wheel left right sideport vis Fig 57 4 Selects beam splitting for right sideport doc left sideport doc and visual vis observation...

Page 85: ...objective positions in manual coded or motorized versions Manual and coded nosepieces rapid objective change by rotating the nosepiece using the adjustment ring Fig 63 3 Motorized nosepiece rapid obje...

Page 86: ...ith 2 position filter changer or polarizer D rotatable with color glass holder Fig 64 4 The polarizer and filter positions can be swiveled into and out of position separately There are stops for the s...

Page 87: ...optical path The short designation for the turret position e g H is displayed on the front of the turret facing the user The condensers 0 35 and 0 55 for phase contrast require the use of one 1 5 mm A...

Page 88: ...h attachment Z Piezo Rot En Rev 4 Scanning stage 130x100 STEP Scanning stage 130x85 MAT CAN Scanning stage 130x85 mot P CAN and Mounting frame K for mechanical and scanning stages Z gliding stage Slot...

Page 89: ...uate the light in the fluorescence path when using the HBO 100 slot A The FL attenuator has six labeled positions which are selected by turning the control wheel Motorized iris diaphragm slider as lum...

Page 90: ...Coarse adjustment large knob fine adjustment small knob Optional Fine drive flat right or left stand 5 materials always flat left already included in stand TL button Fig 57 24 Pressing briefly switch...

Page 91: ...hototube 45 23 with sliding prism for vis doc Bertrand lens and manual vis shutter Fig 72 Switch the shutter on and off by sliding the rotary sliding button Fig 72 1 100 vis 0 vis light shutter Bertra...

Page 92: ...ion of ametropia and can be fitted with eyepiece reticles see also section 4 4 2 LM Set button Fig 57 17 Pressed briefly 1 s Light Manager values are saved Pressed long 2 s Configuration mode is activ...

Page 93: ...t will go Press the clamping lever downwards to lock the stop position LCD display Fig 58 11 On 5 5 materials stand only LCD display with four lines of 16 characters each located on the carrier for tr...

Page 94: ...only Two pairs of buttons Fig 77 1 and 3 and one single button Fig 77 2 for operating motorized components or illumination settings The factory set default button configuration is shown on the adhesiv...

Page 95: ...7 7 materials stands only If it is inconvenient to operate the microscope stand from the right side the functions of the touchscreen TFT display Fig 79 3 on the docking station the right control ring...

Page 96: ...ned below Nosepiece optional only 7 7 materials stand Automatic component recognition for the nosepiece is initiated by pressing the appropriate button on the Settings Components Objectives screen on...

Page 97: ...polarization contrast Oil Immersion objective for oil as immersion medium EC Enhanced Contrast LD Long distance i insulated iHMC improved Hoffman Modulation Contrast LCI Life Cell Imaging alpha Apertu...

Page 98: ...45 mm of all other objectives Insert the corresponding Coverglass cap 422904 9000 000 Fig 81 1 onto the objective to adapt LD A Plan objectives to thin bottoms or cover slips Using corr objectives The...

Page 99: ...ve and focus After each experiment the immersion oil should be removed from the objective using a soft cloth possibly with petrolether Excessive quantities of immersion oil can get into the mechanical...

Page 100: ...the 3 position filter changer Fig 83 5 out of the optical path Place a high contrast specimen on the microscope stage Fig 83 10 Match the eyepiece distance interpupillary distance to the user s indivi...

Page 101: ...sary refocus on the specimen using the fine focusing drive Adjust the light intensity using the rocker switch The field size and objective aperture change each time the objective is changed so that fo...

Page 102: ...der and FL attenuator discrete 10 Microscope stage with inserted universal mounting frame K 11 LM Set button 12 Analyzer slider slot 13 Nosepiece 14 Compensator mount 6x20 only for materials stands 15...

Page 103: ...e focus drive Fig 85 9 to focus on the selected detail of the specimen Should no light be visible in the eyepieces switch on the microLED or HAL 100 illuminator using the RL switch Fig 85 10 Set the l...

Page 104: ...OPERATION ZEISS Brightfield reflected light microscopy Axio Observer 104 431004 7244 001 12 2016 Fig 85 Axio Observer 5 materials example...

Page 105: ...or switching the LED halogen illuminator or the reflected light shutter fluorescence on and off 11 Control wheel for LED light halogen illuminator intensity control 12 Rotary sliding button for vis do...

Page 106: ...permanently saves the current brightness value of the objective in use i e the last value saved using the LM set button is set again after switching off the microscope and switching it on again Separa...

Page 107: ...Aperture HAL TL LED TL HAL RL LED RL None RL TL None Off Smart Classic RL TL RL TL Off List of FL filter sets List of fluorocromes List of contrast modules List of beam splitters List of other filters...

Page 108: ...ing effect To exit configuration mode press and hold the LM set button for at least 2 s Two beeps indicates that the system is exiting configuration mode The LCD display returns to status display mode...

Page 109: ...e switch is on RL for reflected light A brightness value is stored for each nosepiece position TL RL toggle switch is on TL for transmitted light A brightness value is stored for each nosepiece positi...

Page 110: ...anager function The Light Manager is activated CLASSIC or SMART or deactivated OFF when the microscope is switched on depending on the configuration In transmitted light the following parameters are s...

Page 111: ...the user can select his or her own optimum illumination settings for each magnification each combination of objective and Optovar The illumination must be set for all contrast techniques used H PH DIC...

Page 112: ...can be permanently saved by pressing the LM Set button on the right side of the stand Permanently saved settings can be changed temporarily during a session 5 9 4 Selecting the Light Manager mode on 5...

Page 113: ...o overwrite the manufacturer s default settings 5 10 Contrast Manager on the Axio Observer 7 7 materials The Contrast Manager is only available on the 7 7 materials stand It is used for rapid switchin...

Page 114: ...generally split into the following main areas see Fig 92 5 11 1 1 Navigation bar The navigation bar on the left side of the screen contains buttons for navigating between pages The buttons displayed d...

Page 115: ...g the contrast technique The contrast techniques available depend on the current microscope configuration The following contrast techniques may be available Abbr Procedure FL Fluorescence BF Brightfie...

Page 116: ...has different tabs depending on the stand type Bio Med or MAT set under Settings User Stand type see page 140 The menu overview below shows both versions The first level buttons shown on the extreme...

Page 117: ...jectives Colibri LEDs Reflector Virtual reflector Optovar Light path F A Bio Med Automatic see page 126 Soft keys XYZ see page 127 Position Measure Definite Focus Incubation see page 130 Incubation Y...

Page 118: ...was switched off the new components will require configuration after switching on see also section 5 11 9 All menu pages can be accessed using the buttons on the navigation bar on the left The middle...

Page 119: ...on as been reached the Load position pop up window Fig 97 containing the following controls appears Moves the nosepiece back to the working position Moves the nosepiece upwards towards the working pos...

Page 120: ...following settings are adapted in this basic state Bio Med stand transmitted light illuminator is set to medium brightness 3 V aperture diaphragm is opened TL shutter open RL shutter closed Motorized...

Page 121: ...pe Control The Microscope Control page can contain the following tabs depending on the optional configurable motorized components Objectives only Bio Med stands Colibri LEDs Reflector Virtual reflecto...

Page 122: ...t the process to the new objective i e the condenser and reflector turret positions may change contrast adjustment If the contrast technique is not available for the objective the system will switch t...

Page 123: ...uide for the filter wheel excitation 8 pos mot and dual filter wheel mot 452358 7044 001 5 11 5 5 Optovar This tab will not be displayed if there is no motorized Optovar turret installed If an equippe...

Page 124: ...s displayed in the operating section to represent the light path The configuration is determined during initialization of the microscope Two mirrors sideport frontport baseport are active The active l...

Page 125: ...and where applicable the following additional information is displayed Oil Oil immersion objective If an equipped Optovar turret is fitted the available magnifications are displayed Touch the button f...

Page 126: ...figured up to six controls for reflector positions 1 to 6 will be displayed To swivel in the desired reflector module press the relevant button To switch back or forth press the or button 5 11 6 Micro...

Page 127: ...bs Position Measure and Definite Focus 5 11 7 1 Position The controls area of the Position tab is divided into three blocks If you are not using a motorized stage the XY controls are replaced by a Sta...

Page 128: ...ordinates have already been assigned to a button the XYZ values will be displayed otherwise the position number will be displayed Save the current position by touching one of the position buttons If c...

Page 129: ...tion and a defined position Distance between two defined positions If the Z distance shall be measured activate the incl Z button Move the stage to the initial position Press the Start button On doing...

Page 130: ...ctual top temperatures for temperature control Click on the appropriate button to call up the corresponding pop up window e g H Insert P In this window you can switch the temperature control on or off...

Page 131: ...iece fittings using this tab Up to six buttons are displayed Before any objectives have been configured the buttons are labeled only with the numbers of the nosepiece positions After an objective posi...

Page 132: ...ent objective selection Select the relevant nosepiece position and confirm by pressing Yes Press the Save button to save the objective configuration for the selected nosepiece position or press Cancel...

Page 133: ...ing data is displayed designation Type reflected light module RL transmitted light position module TL Once a reflector has been assigned to a position the corresponding reflector button on the Microsc...

Page 134: ...After an objective is assigned to a specific button the magnification appears on the left blue half of the button The right gray part of the button shows the focus speed To change the focus speed for...

Page 135: ...e highest to the lowest magnification Then proceed with all immersion objectives from the highest to the lowest magnification Press the Next Objective button to rotate the nosepiece to the next object...

Page 136: ...tem detects the status of the ports during initialization and when the Settings Components page is opened To assign an adapter to a port press the gray button The Select Camera Adapter list will be di...

Page 137: ...re the selected setting Press the Cancel button to close the window without any selection Illumination type This is used to enter the type of illuminator you are working with halogen illuminator or LE...

Page 138: ...used to select between Standard and Personal settings modes In Standard mode all default functions factory set are active In Personal settings mode administrator defined settings are active for the fo...

Page 139: ...rop down list Use the buttons to select a function from the list Only functions which are actually available with the current microscope configuration are listed Press Save to assign the required func...

Page 140: ...arted automatically 5 Language This tab can be used to select the TFT display language At present the available languages are English and German Changes to this setting will take effect once the micro...

Page 141: ...to activate or deactivate the oil stop function The oil stop function prevents a dry objective from being moved into the immersion fluid The nosepiece is always lowered when switching between a dry a...

Page 142: ...hernet connection 5 Misc The Misc tab is used to calibrate the TFT display Once the TFT Calibration button has been pressed crosses will appear in various positions A blunt pencil should then be press...

Page 143: ...can adapt the TFT display brightness using the buttons Press and hold Display on the navigation bar for at least one second to dim the TFT display Touching anywhere on the TFT display again switches...

Page 144: ...ontrast techniques Axio Observer 144 431004 7244 001 12 2016 5 12 Illumination and contrast techniques 5 12 1 Setting up transmitted light bright field according to K HLER Fig 148 Setting transmitted...

Page 145: ...asDIC module and analyzer 25 Slot for DIC PlasDIC slider 26 Reflector turret coded or motorized 27 Optovar turret selector wheel max 3 positions 28 Coarse fine focus drive right side 29 Control ring r...

Page 146: ...turret if available to a position which contains no filter combination Ensure that it clicks into position correctly If necessary remove the analyzer slider from the slot or move to the open position...

Page 147: ...gm just disappears from the field of view Fig 149 D To set the aperture diaphragm remove one eyepiece from the eyepiece tube and adjust the aperture diaphragm of the condenser to approximately 2 3 the...

Page 148: ...ell components bypass this optical channel and their phase is determined by differences in refractive index and thickness of the specimen In the intermediate image plane these two beams interfere and...

Page 149: ...t perfect Fig 151 A the bright stop must be centered using two 1 5 mm Allen keys Fig 150 1 until it is completely congruent with the dark phase plate Fig 151 B Finally remove the centering telescope f...

Page 150: ...with DIC prisms DIC I DIC II DIC III or DIC condenser module Polarizer e g polarizer D with 2 position filter changer only required where the condenser used does not have a DIC prism with integrated...

Page 151: ...and contrast techniques ZEISS 12 2016 431004 7244 001 151 1 Polarizer D fixed optional rotatable 2 Condenser 3 DIC slider 4 Analyzer slider fixed 5 30 analyzer slider Fig 152 Components required for t...

Page 152: ...d lens a diagonal black line on the DIC slider left top to bottom right will be visible Move the diagonal black line to the center of the field of view by adjusting the knurled screw on the DIC slider...

Page 153: ...LD Plan Neofluar 20x 0 4 Corr M27 LD Plan Neofluar 40x 0 6 Corr M27 LD Plan Neofluar 63x 0 75 Corr M27 At least one of these objectives must be available Condensers LD condenser 0 35 H Ph0 Ph1 Ph2 DIC...

Page 154: ...PlasDIC module combines a DIC prism with polarizer and analyzer and thus replaces individual PlasDIC sliders with polarizer for each objective and a separate analyzer or PlasDIC slider for LD A Plan 1...

Page 155: ...ce If the PlasDIC module has not yet been fitted into the 3 position contrast slider Loosen the screws Fig 153 1 2 on the cover Fig 153 1 1 of the contrast slider and remove the cover Insert the PlasD...

Page 156: ...ION ZEISS Illumination and contrast techniques Axio Observer 156 431004 7244 001 12 2016 5 12 5 Setting up reflected light bright field Fig 154 Setting reflected light brightfield Axio Observer 5 mate...

Page 157: ...Holder with LCD display 1 Application The reflected light bright field microscopy is the simplest and most widely used microscopy technique which is used to examine opaque samples or specimens e g pol...

Page 158: ...A Turn the focus drive Fig 154 9 to refocus on the edge of the luminous field diaphragm Fig 155 B and use the centering screws Fig 156 2 3 to center the luminous field diaphragm with the edge of the f...

Page 159: ...itted with fluorescence filter set in FL P C reflector module or FL filter set fitted in dual filter wheel mot and filter wheel excitation 8 pos mot Optional Fluorescence illuminator HXP 120 V HBO 100...

Page 160: ...ng the lever Fig 156 4 close the diaphragm until it becomes visible in the field of view Center the diaphragm using the two adjusting screws Fig 156 2 3 on the slider using a 3 mm ball headed screwdri...

Page 161: ...ich otherwise would be unavoidable 2 Instrument equipment Axio Observer materials with attached microLED or adjusted HAL 100 illuminator EC Epiplan Neofluar EC Epiplan objectives Fixed analyzer slider...

Page 162: ...ver 5 materials and 7 materials stands must also be equipped with one of the following reflected light illuminators since only they have strain free optics Reflected light illuminator HD POL FL 423608...

Page 163: ...ment equipment Axio Observer materials with attached microLED or adjusted HAL 100 illuminator EC Epiplan Neofluar or EC Epiplan objectives additionally labeled with HD ACR P C dark reflector module 3...

Page 164: ...eflector module CDIC P C DIC Pol P C reflector module or DIC Pol Red I P C in reflector turret or C DIC TIC P C reflector module in combination with C DIC slider 6x20 3 Reflected light DIC Prepare the...

Page 165: ...terest is visible at maximum contrast Additional contrasting effects can be produced by adjusting the selector wheel on the C DIC slider 5 Additional note Differential interference contrast or differe...

Page 166: ...microscope as described in section 5 12 5 for reflected light brightfield Swivel in the C DIC TIC P C reflector module on he reflector turret Fig 154 7 into the beam path Insert TIC slider 6x20 see F...

Page 167: ...e is aperture dependent and decreases with increasing illumination aperture Accordingly the following correction values must be taken into account depending on the objective used Objective Correction...

Page 168: ...tire object with a homogeneous layer and then measuring the path difference If the layers or steps are transparent such as silicon dioxide on silicon the interference fringes may change their color De...

Page 169: ...Observer materials camera outputs The descriptions are based on an object such as a stage micrometer with readable figures or letters to illustrate the orientation Original object Viewed without micro...

Page 170: ...vis 50 doc 0 vis 100 doc 425535 0000 000 Eyepieces 100 vis Binocular ergotube 425150 0000 000 20 vis 80 doc Sideport 60N left 2 switch positions 425151 0000 000 0 vis 100 L Sideport 60N left 2 switch...

Page 171: ...vis 100 doc right 000000 1069 228 100 frontport Beam path switching 00 1069 229 100 frontport Beam path switching mot 425126 0000 000 100 doc baseport Use of camera adapters which work without using...

Page 172: ...n working with microphotographic devices consult the corresponding manuals of the cameras as well Fix the camera adapter 60N Fig 161 4 or 5 to the camera Remove the dust cap from the camera port The t...

Page 173: ...ra lens from the camera housing Fig 162 1 Attach appropriate T2 adapter Fig 162 2 to the camera housing Remove the dust cover from the V200 T2 2 5x adapter for SLR Fig 162 3 Screw the V200 T2 2 5x for...

Page 174: ...al camera e g Axiocam digital camera from ZEISS Fig 163 1 to the frontport via the V200 C 2 3 0 63x video adapter Fig 163 2 Attaching the video adapter is analogous to the photo adapter Attach the pre...

Page 175: ...ola etc by breathing on it and wiping with a dust free cotton cloth or a moistened cloth You may add a mild detergent to the water Remove stubborn oily or greasy dirt immersion oils finger prints usin...

Page 176: ...evailing national rules for accident prevention Eliminate contaminations on the instrument according to the rules for prevention of accidents Switch off the instrument each time after use and place th...

Page 177: ...5 materials The fuse compartment is integrated into the power socket and contains two T 5 0 A H 250 V 5 x 20 mm fuses If the device fuses fail the cause must first of all be ascertained and technical...

Page 178: ...the external power supply unit VP232 2 The fuse compartment of the VP232 2 power supply unit is located at the rear of the unit and contains two type T 4 0 A H 250 V fuses Pull out the power plug Pull...

Page 179: ...lder is integrated into the power socket and contains two T 2 0 A H 250 V 5 x 20 mm fuses If the device fuses fail the cause must first of all be ascertained and technical problems properly remedied P...

Page 180: ...nstrument or any work on the power supply may only be carried out by service technicians or specially authorized personnel If servicing is required please contact your local representative or Carl Zei...

Page 181: ...mitted light cover slip thickness d Diameter DC Direct current DIC Differential Inference Contrast DIN Deutsches Institut f r Normung German Standards Institute doc Documentation EC European Community...

Page 182: ...an PlasDIC Plastic differential interference contrast RL Reflected light SLR Single lens reflex SW Wrench size across flats T Slow blow fuse type TIC Total Interference Contrast in circularly polarize...

Page 183: ...canning stage 130x85 mot P CAN bottom side 45 Fig 24 Scanning stage 130x85 mot P CAN top side 46 Fig 25 Scanning stage 130x85 mot P CAN connections on bottom side 46 Fig 26 Fitting the scanning stage...

Page 184: ...der with LCD display 94 Fig 77 Control ring right view from rear 94 Fig 78 Control ring left view from rear 95 Fig 79 Docking station with TFT display control ring and focusing drive 95 Fig 80 Objecti...

Page 185: ...lector page 133 Fig 124 Settings Components Reflector Configure reflector position in Reflector turret page 133 Fig 125 Settings Components Focus page 134 Fig 126 Settings Components Focus Set parfoca...

Page 186: ...cted light 158 Fig 157 Slot for Polarizer slider RL 6x30 mm 90 rotatable 162 Fig 158 DIC C DIC reflected light components on Axio observer materials 164 Fig 159 TIC slider 6x20 166 Fig 160 Interferenc...

Page 187: ...m splitter 57 Binocular section 92 Binocular tubes 91 Brightfield 144 C Care 175 Carrier for transmitted light illumination 40 C DIC 164 Changing the fuse 179 Checks 176 Colibri 7 69 Colibri 2 69 Coli...

Page 188: ...Fluorescence illuminator 160 Focusing drive 84 90 Function controls 72 G Gliding stage Z 50 H Halogen illuminator 65 Halogen lamp 67 HBO 100 illuminator 68 69 Heatable microscope stage S1 50 HXP 120 V...

Page 189: ...on controls 72 84 Operation 71 Optovar turret 92 P Phase contrast 148 Photomicrography 173 174 PlasDIC contrast 153 Polarization 161 R Reflected light 156 159 161 163 164 166 Reflector turret 54 90 RL...

Page 190: ...dex Axio Observer 190 431004 7244 001 12 2016 U Unpacking 37 V Vertical adjustment for condenser 86 Vertical stop for focus drive 93 Videomicroscopy 174 Virt Reflector 123 W Warranty information 12 X...

Page 191: ...nt rights ZEISS 12 2016 431004 7244 001 191 7 4 Patent rights Instruments instrument components or methods described in this manual are protected by patents DE29821694 JP3032901 US6392796 US5015082 US...

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