OPERATION
Axio Observer
Using objectives
ZEISS
12/2016
431004-7244-001
99
Using immersion objectives
With immersion objectives, the air between the cover slip and the objective is replaced with a liquid, the
so called immersion oil.
Place a small, bubble-free drop of Immersol 518 N
®
(for transmitted light applications) or 518 F
®
(for
fluorescence) on the front lens of the objective and insert the culture vessel or specimen with the cover
slip pointing towards the objective onto the specimen stage or into the mounting frame.
Then carefully approach the objective and focus.
After each experiment, the immersion oil should be removed from the objective using a soft cloth
(possibly with petrolether).
Excessive quantities of immersion oil can get into the mechanical components of inverted microscopes
and reduce their functionality.
ATTENTION
Observe the safety instructions for using immersion oil on page 8.
Cleaning instructions can be found in the brochure “The Clean Microscope”.
Using autocorr objectives
When using the autocorr objectives, observe the quick start guide “Installation and configuration of
autocorr objectives” (420852-7144-001)
Objectives to be used preferably with Axio Observer
Objectives to be used preferably with Axio Observer microscopes can be found on the following
website:
Summary of Contents for Axio Observer Series
Page 1: ...Axio Observer Inverted microscope Operating manual...
Page 192: ......
