Image Acquisition with the AxioCam MRc5
AxioCam MRc5 Instal Reference
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For this calibration process make sure that the camera is viewing a completely
black image. For example, close the light path to the camera on the
microscope. In exceptional cases it may be necessary to remove the camera
from the microscope for this calibration process and to cover the sensor with
the cap provided.
Calibration takes approx. 20 seconds. At the end of this period it will continue
to be available even if the software is restarted. Under certain circumstances it
may be necessary to repeat this calibration process from time to time.
NOTE:
The black reference is needed to correct deviations in the dark current for
individual pixels. This measure is additional to the camera’s available
Peltier cooling, and allows a uniformly dark and even image background
to be achieved, even with long integration times.
Activating the black reference can reduce the maximum possible speed of
the live image due to additional computations.
White Reference
Activate this setting to compensate a decrease of image brightness to the
borders of the view area.
For this calibration process ensure that the camera is exposed to a completely
empty image. To do so place the sample to a completely empty position of
the slide.
NOTE:
Use a clean slide, a clean objective and
defocuse your microscope
in order
to avoid the footprint of dust particles. Ensure that the microscope is
properly adjusted (Köhler illumination).
After changing the objective a white reference must be done.
Activating the white reference can reduce the maximal possible speed of
the live image.
