Image Acquisition with the AxioCam MRc5
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In the case of fluorescence, however, only very narrow-band light is emitted.
This is received only by the color pixels that are sensitive to it. Consequently,
the resolution of images of this type is reduced in comparison with white
light.
As fluorescence samples can be subject to bleaching during exposure, there is
a risk that the image intensity of images in “quality” mode may diminish from
one component image to another. This can lead to artifacts in the final image.
Check if your fluorescence sample will bleach out within the necessary
exposure time. If it will, you will have to revert to "Fast" mode with 1292 x
968 pixels.
A way to reduce the effects of bleaching out is to reduce the exposure time
required by the camera by increasing the gain.
Configurable toolbars for operating the camera
The AxioVision software allows you to configure your own toolbars. This
means that the procedure can be tailored to your own requirements, and thus
optimized. The size of the toolbar buttons can also be adjusted, helping you
to work quickly with the mouse. A detailed description of how to create
toolbars can be found in chapter 9 "Configuration" - "Adapting Toolbars" in
the AxioVision Manual.
