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Axiolab 5
Mounting standard components
ZEISS
05/2019
430037-7444-001
61
3.1.7.6
Centering the objectives of the
polarization stand
The nosepiece 5x Pol is equipped with one fixed
and four centerable objective positions.
Stage centering of the non-centering objective
mount is necessary to ensure that a specimen
feature located in the center of the field of view
does not drift out while rotating the stage. By
centering of the remaining objectives, the
specimen feature remains in the center of the field
of view even after changing the objective.
•
The KÖHLER illumination on the microscope
must be adjusted before centering the stage
(see section 4.2.1).
•
For centering the stage, use a contrasting
specimen and an eyepiece with crossline reticle.
•
First, turn the nosepiece to swing the non-
centering objective mount. Center the rotary
stage for the non-centering objective mount as
described under 3.1.7.5.
•
Turn the nosepiece to move a centering objective mount into the beam path.
•
Rotate the stage to determine the position of maximum offset of the specimen feature (Fig. 3-12/
3
,
origin of the arrow) from the center of the eyepiece reticle.
•
Reset the two centering screws on the stage carrier (Fig. 3-12/
2
) using one AF 1.5 Allen wrench
1
) each to move the specimen detail by half the arrow length in the direction of the
crossline center. Check whether the specimen detail moves when the stage is rotated again; repeat
the procedure if required.
•
Center the other three objectives in the same manner.
NOTE
To maintain this centering accuracy, when replacing an objective, do not hold the objective
itself; hold the knurled ring of the nosepiece to rotate it.
Fig. 3-12
Centering objectives
