OPERATION
ZEISS
Illumination and contrast methods in transmitted light
Axiolab 5
88
430037-7444-001
05/2019
•
If the overlap is not exact (
A
), the
lighter annular diaphragm must be recentered
with the aid of two Allen wrenches (AF 1.5)
(
1
and
4
). Adjust the two centering
screws (
2
and
3
) so that you achieve a
full overlap with the darker phase ring
(
B
).
•
Remove the auxiliary microscope from the tube
and replace it with the eyepiece.
In order to increase the image contrast, an
interference broadband filter, green 32 x 4, may be
mounted on the field diaphragm or inserted into
the color glass carrier (if available).
A complete phase contrast can only be achieved
when the light-colored annular diaphragm (in the
condenser) overlaps exactly with the dark-colored
phase ring (in the objective) in the illumination
beam path (
B
).
Fig. 4-6
Centering the annular phase
diaphragm (light-colored, in the
condenser) and the phase ring
(dark-colored, in the object)
