Zeiss Axiolab A, Operating Manual

Page 1: ...Axiolab A Reflected Light Microscope Operating Manual...

Page 2: ...e service Unless expressly authorized forwarding and duplication of this document utilization and communication of its contents are not permitted Violations will entail an obligation to pay compensati...

Page 3: ...pieces 1 19 1 7 Stage Micrometers and Eyepiece Reticles 1 20 1 8 Technical Data 1 22 2 START UP 2 1 2 1 Unpacking the Instrument 2 3 2 2 Screw in Objectives 2 3 2 3 Insertion of Eyepieces 2 4 2 3 1 In...

Page 4: ...LER Illumination 3 14 3 3 Attachment of Microscope Stages and Specimen Holders 3 15 3 3 1 Attachment of Pol Rotary Stage 3 16 3 4 Use of Polished Section Attachment 3 17 3 5 Photomicrography and Video...

Page 5: ...ight brightfield 3 4 Fig 3 3 Setting of reflected light polarization 3 5 Fig 3 4 Centering of objectives 3 6 Fig 3 5 Setting of transmitted light polarization 3 7 Fig 3 6 Determine the n vibration dir...

Page 6: ...the figure in Section 2 with the serial number 7 In each figure details discussed in the text have been assigned with a reference line marking and an item number In the running text Mains cable 2 7 1...

Page 7: ...quirements of the EC directive 89 336 EC and the EMC legislation of November 9th 1992 This operation manual includes information and warnings which must be observed by the user The following warning a...

Page 8: ...nt operation Please contact your local Carl Zeiss service agency or the Carl Zeiss microscopy service for the repair of the instrument The wide range power unit which is integrated in the stand of the...

Page 9: ...ranty The manufacturer guarantees that the instrument has no material and production defects when delivered You must inform us of any defects immediately and we must do anything to minimize the damage...

Page 10: ...Carl Zeiss Axiolab A X B 40 015 e 06 99 Overall View of the Axiolab A Reflected Light Microscope...

Page 11: ...1 1 Name Intended Application 1 2 1 2 Instrument Description 1 2 1 3 Microscope Configurations and Modules 1 5 1 4 Function Elements see Fig 1 5 after this table 1 12 1 5 Objectives 1 18 1 6 Eyepiece...

Page 12: ...outine microscope Due to its compact design and clearly arranged controls the instrument also meets high demands on user friendliness Microscope stand 1 1 1 diecast component with low lying center of...

Page 13: ...the glide stage can be locked in a top or a bottom stop The maximum specimen height is 45 mm The mechanical stage is a component of the transmitted light version of the Axiolab A and permits the parti...

Page 14: ...levant control The correct color temperature for color photography 3200 K is automatically reached in the end stop The XBO 75 or HBO 50 illuminators can also be attached to the Axiolab A for examinati...

Page 15: ...phy videomicroscopy Switchover is 100 in each case i e simultaneous observation and documentation is not possible The use of specific sliders in the reflected light illuminator H allows observation in...

Page 16: ...Carl Zeiss Axiolab A 1 6 06 99 B 40 015 e Fig 1 3 Axiolab A microscope configurations...

Page 17: ...20 Br foc 444232 9902 000 3 1 Epiplan objective 5 0 13 442920 0000 000 3 2 Epiplan objective 10 0 20 442930 0000 000 3 3 Epi objective 20 0 40 0 442941 0000 000 3 4 Epiplan objective 50 0 70 0 442950...

Page 18: ...Carl Zeiss Axiolab A 1 8 06 99 B 40 015 e Fig 1 4 Axiolab A accessories 1 of 2...

Page 19: ...5 Epiplan Neofluar objective 20 0 50 0 442340 0000 000 2 6 Epiplan Neofluar objective 50 0 85 0 442350 0000 000 for covered specimens 2 7 Achroplan objective 20 0 45 0 17 440040 0000 000 2 8 Achropla...

Page 20: ...Carl Zeiss Axiolab A 1 10 06 99 B 40 015 e Fig 1 4 Axiolab A accessories 2 of 2...

Page 21: ...00 9 2 1 Mains cable with European plug 380137 6750 000 9 3 35 mm Mot DX film cassette 456071 0000 000 9 4 Adapter 60 for microscope camera d 30mm 456006 0000 000 9 5 Projection lens P 2 5 for MC 80 M...

Page 22: ...yepieces are required if reticles or photo reticles are used 8 Dust protection slider 2 pcs These two dust protection sliders should always remain in the Axiolab A microscope technique sliders can als...

Page 23: ...21 Pushrod for luminous field diaphragm incident light Permits insertion of a perforated plate with 1 3 field size 22 Dust protection slider Prevents the entry of dust Can be replaced during operation...

Page 24: ...nable objects on a 26 76 mm slide to be held in position Objects can also be held in position by the so called polished specimen stage accessory with which unilaterally polished specimens can be align...

Page 25: ...Pushrod to change beam path 19 Aperture diaphragm 20 Filter slider 21 Pushrod for luminous field diaphragm 22 dust protection slider 23 Polarizer slider P 24 Dust protection slider 25 Analyzer slider...

Page 26: ...Carl Zeiss Axiolab A 1 16 06 99 B 40 015 e Fig 1 5 Axiolab A function elements 1 of 2...

Page 27: ...Axiolab A Carl Zeiss B 40 015 e 06 99 1 17 Fig 1 5 Axiolab A function elements 2 of 2 1 1 2...

Page 28: ...0 The objective magnification multiplied with the eyepiece magnification usually 10 results in the visual overall magnification e g 10 10 100 The numerical aperture 1000 e g 0 20 1000 200 is the highe...

Page 29: ...0 0000 000 Epiplan Neofluar 20 0 50 1 4 0 442340 0000 000 Epiplan Neofluar 50 0 80 0 58 0 442350 0000 000 Transmitted light brightfield Achroplan Achroplan 20 0 45 2 07 0 17 440040 0000 000 Achroplan...

Page 30: ...Cat No stage micrometer positive 5 100 100 y D 0 17 mm gradation on the y axis 5 mm in 5 intervals gradation on the y axis 1 mm in 100 100 mm 10 m accuracy 1 m 474026 0000 000 crossline micrometer 14...

Page 31: ...cle MC 2 5 d 26 mm for 35 mm photography with an additional magnification of 2 5 or for large format photography with 10 additional magnification 454075 0000 000 If an eyepiece reticle is used the bin...

Page 32: ...ssible relative humidity max 85 Altitude max 2000 m Atmospheric pressure 800 hPa to 1060 hPa Pollution degree 2 Operating data Category of use closed rooms Protection class I Protection type IP 20 Ele...

Page 33: ...6 V 4 8 V 300 h 2000 h illuminated area 1 7 1 7 mm Opto mechanical data Stage focusing coaxial coarse fine drive lifting range 20 mm coarse drive 1 rotation 4 mm fine drive 1 10 step down ratio Object...

Page 34: ...Carl Zeiss Axiolab A 1 24 06 99 B 40 015 e...

Page 35: ...2 Compensation of Ametropia when Eyepiece Reticles are used 2 5 2 4 Setting of Interpupillary Distance 2 5 2 5 Attachment of Reflected Light Halogen Illuminator 2 6 2 6 Retrofit the Transmitted Light...

Page 36: ...Carl Zeiss Axiolab A 2 2 B 40 015 e 06 99...

Page 37: ...worktable Remove the plastic sleeve 2 1 4 from the instrument Remove the adhesive strips which secure the various controls during transport Leave dust protection and technique sliders 2 1 2 5 6 insert...

Page 38: ...nder 1 6 The slight image shift caused by the additional path through the glass is taken into account on the diopter scale by the fact that the zero point position is indicated not by the white dot 2...

Page 39: ...image of a specimen via the focusing drive by looking through the eyepiece with reticle When the image and the eyepiece reticle are in focus in the above eyepiece focus the image for the second eye vi...

Page 40: ...he Axiolab A is possible with the configuration described in section 1 3 The pure reflected light versions of the Axiolab A can also be upgraded for transmitted light observation by installing the Ret...

Page 41: ...smitted light operation press the A D converter 1 5 13 at the rear of the Axiolab A into the lower position The functions of the on off switch 1 5 28 and the illuminance control 1 5 27 are retained re...

Page 42: ...lecting reflected light specimens 2 9 Connecting the Instrument to the Line The microscope can be operated using line voltages of 100 240 V without conversion Connect the line cable 2 7 1 with connect...

Page 43: ...zation with extended Polarization Equipment 3 5 3 2 4 Setting of Epi Fluorescence 3 13 3 2 5 Setting of Transmitted Light Brightfield K HLER Illumination 3 14 3 3 Attachment of Microscope Stages and S...

Page 44: ...Carl Zeiss Axiolab A 3 2 B 40 015 e 06 99...

Page 45: ...on off switch is switched off Set the required image brightness via the illuminance control 3 1 5 Depending on what is required insert attenuation or conversion filter in the filter slider 3 1 3 and...

Page 46: ...1 and set the eyepiece distance and interpupillary distance again as required also see section 2 4 The aperture diaphragm 3 2 4 must be set to approx half the size of the opening diameter by turning...

Page 47: ...analyzer slider to optimum contrast maximum dimming in the field of view via the adjustment lever If possible this analyzer position should not be changed Reduce illumination aperture by turning the...

Page 48: ...the drilled centering holes on the nosepiece Turn the specimen stage and search for the apparent center of rotation cross 3 4 1 The center of rotation is also the center of the stage rotation Use the...

Page 49: ...ightfield examinations in transmitted light Push in the plane glass pushrod 3 5 3 Swing in polarizer 3 5 6 and set it to 0 Push in analyzer 3 5 1 until stop so that the field of view is dark When a 5...

Page 50: ...the diagnosis of biocrystals gout pseudogout Settings Set the microscope as usual for transmitted light brightfield examinations Turn stage until the first stop position unlock stop and turn the stag...

Page 51: ...and n of the object are parallel Therefore n of the object also lies in NE SW direction in the case of a higher interference color and is oriented parallel to the longitudinal axis of the fiber Summar...

Page 52: ...e optical axis of uniaxial crystals or one of the optical axes of biaxial crystals is almost parallel to the observation direction Move one such crystal into the center of the switchable crosslines Th...

Page 53: ...llow optically negative blue optically positive Biaxial Crystals If biaxial crystals show a cross in conoscopic observation which is resolved into two hyperbola legs when the stage is turned the acute...

Page 54: ...e as usual for examinations in reflected light brightfield see section 3 2 1 Pull out plane glass pushrod 3 3 1 Insert polarization slider 3 3 3 until stop Insert analyzer slider 3 3 4 adjusting lever...

Page 55: ...with the 6 V 30 W halogen illuminator 3 9 1 and the standard brightfield beam splitter The equipment consists of a special FL filter slider 3 9 2 which is inserted in the reflected light illuminator H...

Page 56: ...image of the luminous field diaphragm appears in focus Use the centering screws 3 10 2 to move the luminous field diaphragm to the center of the field of view Open the luminous field diaphragm until...

Page 57: ...f opaque objects the mechanical stage can be equipped with the specimen holder A 3 11 6 The stages can be changed by the users themselves Loosen hexagonal screw 3 12 2 on the right side of the stage u...

Page 58: ...onally opposite to each other or parallel The Pol object guide 3 13 11 accessory 473325 0000 000 provides a more convenient possibility It is inserted in the drilled holes 3 13 7 and secured using the...

Page 59: ...olished surface and aligns them parallel to the stage surface Gliding stage 3 14 6 or also fixed stage at the lower stop Remove magnetically held plate 3 14 1 from the polished section stage 3 14 4 Re...

Page 60: ...led out for photomicrography or videomicroscopy Since this is a 100 changeover simultaneous observation and photography is not possible Special adapters allow commercially available 35 mm cameras or m...

Page 61: ...unit A in the camera tube Align the camera unit in the required position and tighten the three hexagonal screws 3 16 6 Pull out pushrod 3 16 7 completely for photomicrography When artificial light co...

Page 62: ...dapter for OLYMPUS OM OM bayonet 416002 0000 000 T2 adapter for MINOLTA SR bayonet 416003 0000 000 T2 adapter for CANON FD bayonet 416004 0000 000 T2 adapter for NIKON F bayonet 416009 0000 000 T2 ada...

Page 63: ...and fix it by clamping ring 3 17 3 anticlockwise Attach 35 mm film cassette Mot DX 3 17 1 to the basic body in such a way that the contact pins firmly engage in the relevant sockets Pull out pushrod...

Page 64: ...5 60 C 2 3 1 0 456107 456107 60 C 2 3 0 63 456106 456106 60 C 1 2 0 5 456119 456119 60 C 1 3 3CCD 0 5 456108 456108 60 C 1 3 0 4 456123 456123 Zoom 60 C 2 3 0 4 2 456115 456115 60 ENG 2 3 1 0 456117 4...

Page 65: ...ayonet of the video camera and clamp it tight Insert premounted unit video camera with video adapter or video zoom adapter in the camera tube of the Axiolab A align it and fix it using the three hexag...

Page 66: ...ng area DIN A4 notepad lying in front of the stand appears symmetrically aligned also in the field of view Screw on the drawing eyepiece Illuminate the drawing area in such a way that it can be seen i...

Page 67: ...b A Carl Zeiss B 40 015 e 06 99 4 1 CARE TROUBLESHOOTING AND SERVICE Contents 4 CARE TROUBLESHOOTING AND SERVICE 4 3 4 1 Maintenance of the Instrument 4 3 4 2 Troubleshooting 4 4 4 3 Requesting Servic...

Page 68: ...Carl Zeiss Axiolab A 4 2 B 40 015 e 06 99...

Page 69: ...sten the cloths with petroleum ether Clean the front lenses of the objectives using petroleum ether but do not use alcohol When using the Axiolab A in humid climatic zones proceed as follows Store the...

Page 70: ...aragraph 2 or 3 1 Check the Line Cable and Fuses and Change Fuses Make sure to disconnect the instru ment from the line first Check line cable 4 1 1 and replace it if required Use 2 0 mm watchmaker s...

Page 71: ...ether with the accessories Loosen both fixation screws 4 2 5 by turning them to the left and remove the carrier plate 4 2 4 containing the lamp Remove new 6 V 30 W halogen lamp from the packaging and...

Page 72: ...or the first time to prevent dirt from burning in Make sure that the lamp carrier plate exactly fits the positioning pins Attach lamp insert again by slightly pressing the handle together Attach venti...

Page 73: ...Axiolab A Carl Zeiss B 40 015 e 06 99 A 1 Annex List of Abbreviation A 3 Certification in Accordance with DIN EN ISO 9001 DIN EN 46001 A 5 EC Conformity Declaration A 7...

Page 74: ...Carl Zeiss Axiolab A A 2 B 40 015 e 06 99...

Page 75: ...mpatibility EN European standards ENG Electronic News Gathering E PL name of eyepiece type Epiplan FAA free working distance FL fluorescence foc focusing HBO mercury vapor short arc lamp HF brightfiel...

Page 76: ...Carl Zeiss Axiolab A A 4 B 40 015 e 06 99 T slow blow fuse type TV television T2 Adapter standard adapter for 35 mm cameras...