Axiolab A
Carl Zeiss
B 40-015 e 06/99
3-7
(2)
Transmitted-Light Polarization - Detection of Birefringence
This technique is used for the examination of transparent, birefringent objects. Birefringence can be
recognized, with crossed polarizer and analyzer, by the otherwise dark field of view being brightened 4
times when the specimen stage is rotated about 360°. Respective of birefringence, thickness and
orientation of the object, the interference colors range from just visible gray (e.g. in biological objects) to
white, yellow, red, blue, etc. and high-order white.
•
Set the microscope as usual for brightfield
examinations in transmitted light.
•
Push in the plane glass pushrod (3-5/
3
).
•
Swing in polarizer (3-5/
6
) and set it to 0°.
•
Push in analyzer (3-5/
1
) until stop so that the
field of view is dark.
☞
When a
±
5° rotary analyzer is used,
the analyzer must be in the center
stop position.
•
Move the object into the field of view and turn
the stage (3-5/
4
) containing the object.
☞
Birefringence is indicated by the either
colorless or colored brightening of the
object. However, optically anisotropic
materials can also remain dark if an
isotropic direction, e.g. of optically
uniaxial or biaxial crystals is oriented
parallel to the observation direction.
The conoscopic viewing method
makes it possible to find out whether
the object is isotropic or anisotropic.
Fig. 3-5
Setting of transmitted-light
polarization
Summary of Contents for Axiolab A
Page 1: ...Axiolab A Reflected Light Microscope Operating Manual...
Page 16: ...Carl Zeiss Axiolab A 1 6 06 99 B 40 015 e Fig 1 3 Axiolab A microscope configurations...
Page 18: ...Carl Zeiss Axiolab A 1 8 06 99 B 40 015 e Fig 1 4 Axiolab A accessories 1 of 2...
Page 20: ...Carl Zeiss Axiolab A 1 10 06 99 B 40 015 e Fig 1 4 Axiolab A accessories 2 of 2...
Page 26: ...Carl Zeiss Axiolab A 1 16 06 99 B 40 015 e Fig 1 5 Axiolab A function elements 1 of 2...
Page 27: ...Axiolab A Carl Zeiss B 40 015 e 06 99 1 17 Fig 1 5 Axiolab A function elements 2 of 2 1 1 2...
Page 34: ...Carl Zeiss Axiolab A 1 24 06 99 B 40 015 e...
Page 36: ...Carl Zeiss Axiolab A 2 2 B 40 015 e 06 99...
Page 44: ...Carl Zeiss Axiolab A 3 2 B 40 015 e 06 99...
Page 68: ...Carl Zeiss Axiolab A 4 2 B 40 015 e 06 99...