Axiolab A
Carl Zeiss
B 40-015 e 06/99
3-13
3.2.4
Setting of Epi-Fluorescence
The fluorescence technique allows fluorescent structures to be visualized with high contrast in a typical
fluorescence color. The examined material can be autofluorescent or may have been mixed with
fluorescent substances (fluorochromes).
The epi-fluorescence technique can be performed using equipment for simple fluorescence examinations
with the Axiolab A, together with the 6 V, 30 W halogen illuminator (3-9/
1
) and the standard brightfield
beam splitter.
The equipment consists of:
- a special FL filter slider (3-9/
2
) which is inserted
in the reflected-light illuminator H instead of
the standard filter slider.
- a dia. 25 excitation filter (3-9/
3
) especially for
the blue excitation of Epodye.
- a dia. 25 barrier filter (3-9/
5
) especially matched
to this excitation filter, which is inserted in one
of the two dust protection sliders (3-9/
6
).
☞
This technique requires much light.
Turn the "illuminance" control (3-9/
7
)
to its right stop, fully open aperture
diaphragm (3-9/
4
) via the adjusting
wheel.
Fig. 3-9
Setting of epi-fluorescence
Summary of Contents for Axiolab A
Page 1: ...Axiolab A Reflected Light Microscope Operating Manual...
Page 16: ...Carl Zeiss Axiolab A 1 6 06 99 B 40 015 e Fig 1 3 Axiolab A microscope configurations...
Page 18: ...Carl Zeiss Axiolab A 1 8 06 99 B 40 015 e Fig 1 4 Axiolab A accessories 1 of 2...
Page 20: ...Carl Zeiss Axiolab A 1 10 06 99 B 40 015 e Fig 1 4 Axiolab A accessories 2 of 2...
Page 26: ...Carl Zeiss Axiolab A 1 16 06 99 B 40 015 e Fig 1 5 Axiolab A function elements 1 of 2...
Page 27: ...Axiolab A Carl Zeiss B 40 015 e 06 99 1 17 Fig 1 5 Axiolab A function elements 2 of 2 1 1 2...
Page 34: ...Carl Zeiss Axiolab A 1 24 06 99 B 40 015 e...
Page 36: ...Carl Zeiss Axiolab A 2 2 B 40 015 e 06 99...
Page 44: ...Carl Zeiss Axiolab A 3 2 B 40 015 e 06 99...
Page 68: ...Carl Zeiss Axiolab A 4 2 B 40 015 e 06 99...
