Zeiss AxioObserver D1, User Manual

4.

Repeat

exposure

time

measurement

for

each

channel,

however,

adjust

the

fine

focus

for

only

one

channel

ie

the

most

important

one

5.

To

capture

your

final

image,

press

the

button

located

at

the

bottom

of

the

C

tab.

Post

‐

Acquisition

Adjustments

Brightness/Contrast

1.

Temporarily

turn

off

the

pseudo

‐

coloring

of

the

displayed

image

by

pressing

the

button.

Each

channel

is

displayed

in

black/white;

you

can

view

one

channel

at

a

time.

2.

Click

on

the

properties

button

found

below

the

image

3.

While

under

Display

tab

of

properties

window,

click

the

Min/Max

button,

or

under

your

image,

press

the

button.

4.

You

can

make

further

adjustments

using

the

displayed

histogram

(ie

move

top

of

line

left,

and

bottom

of

line

right)

‐

NOTE:

do

not

adjust

the

Gamma

5.

Repeat

for

each

channel

6.

Press

the

button

Adding

a

Scale

bar

1.

To

add

a

scale

bar

,

press

the

button,

or

Annotations

from

the

main

menu

and

select

Scale

bar.

2.

Drag

cursor

along

image

to

get

desired

scale

bar

size

Saving

Files

1.

From

the

main

menu,

select

File



Save

as

2.

Save

your

files

in

the

AxioVision

format

(.zvi)

in

the

appropriate

destination



your

lab

folder



your

folder.

Please

refer

to

the

Attention

Users

signs

posted

on

the

wall.

Shut

Down

Procedure

8

If

used

oil,

gently

remove

oil

from

objective

using

neatly

folded

dry

lens

paper

9

Put

10x

objective

in

place,

and

lower

objective

10

Close

AxioVision

program.

Do

not

save

changes

made

to

the

experiment!

11

Shut

down

computer.

Wait

until

completely

turned

off

12

Turn

Microscope

OFF

13

Toggle

Power

Bar

switch

OFF

14

FluoArc

Lamp

:

‐

Regular

work

hours

:

set

intensity

to

50%

on

control

pad

(F2),

and

leave

lamp

ON

‐

Non

‐

work

hours

:

Turn

lamp

OFF

15

Cover

microscope

with

dust

cover,

but

DON’T

COVER

THE

HOT

LAMP

HOUSING!!