OPERATION
Axioscope 5/7/Vario
Illumination and contrast methods in transmitted light …
ZEISS
03/2018
430035-7344-001
107
(2) Setting the microscope for simple conoscopy using the diopter or the auxiliary microscope
•
Set the microscope as for transmitted light brightfield microscopy using the KÖHLER method (see also
section 4.2.1).
•
Put the specimen onto the stage and focus on it.
•
Move the polarizer and the analyzer into the light path.
•
If you do not use the Pol binocular photo tube, carry out the following two steps:
−
first, align the crossline micrometer 14:140 or the eyepiece reticle to the vibration direction of the
polarizer using the Pol adjustment aid. Please refer to section 4.2.6.2.
−
remove the Pol adjustment aid.
•
Move a selected crystal to the center of the reticle. Only crystals above a defined size can be observed.
•
Swivel in the front lens on the condenser, if necessary.
•
For conoscopy of small crystals, close the luminous-field diaphragm, if necessary, to prevent the axial
figure of the examined crystal from being superimposed by the axial figures of adjacent crystals.
•
Swivel the 40x or 50x objective into the light path and focus with the focusing drive.
•
After removing an eyepiece from the tube, you can view the axial figure in the eyepiece tube.
•
For an improved assessment of the axial figure, you can (if available) insert a diopter or an auxiliary
microscope in the eyepiece tube.
4.2.7.3
Conoscopy with Bertrand system module
(1) Microscope configuration
−
Recommended strain-free objectives:
N-Achroplan 50x/0.8 Pol objective or
EC Plan-Neofluar 40x/0.9 Pol objective
−
Axioscope stand for polarization with the Pol P&C Bertrand system module
−
Pol rotary stage
−
Pol binocular photo tube
or
eyepiece with crossline micrometer 14:140 (454060-0000-000)
and
adjustment aid for polarization microscopy (453679-0000-000)
−
Achromatic-aplanatic 0.9 BF Pol condenser or 0.9 Pol condenser
−
Bertrand system module in reflector turret
−
D polarizer (rotatable or fixed)
