OPERATION
ZEISS
Illumination and contrast methods in reflected light …
Axioscope 5/7/Vario
118
430035-7344-001
03/2018
Fig. 4-18) are determined
with the aid of the eyepiece reticle micrometer or
with the micrometer eyepiece.
If you are working with white light (without an
interference filter), you must set
λ
= 550 nm.
When interference filters are used, it is important
to apply the focal point of their wave lengths.
The measured path difference depends on the
aperture and increases with the illumination
aperture.
The following correction values must be considered depending on the objective used:
Objective
Correction factor k
5x/0.15
1.0057
10x/0.25
1.0161
10x/0.30
1.0236
20x/0.4
1.0436
20x/0.50 and 50x/0.75
1.0718
50x/0.60
1.1111
50x/0.75 and 100x/0.75
1.2038
50x/0.80
1.2500
50x/0.90 and 100x/0.90
1.3929
100x/0.95
1.5241
Table 1:
Correction depending on aperture
Example:
a = 11 mm
b = 5 mm
λ
= 550 nm
objective 20x/0.50
nm
mm
mm
nm
a
k
b
SH
134
22
0718
.
1
5
550
2
=
⋅
⋅
=
⋅
⋅
=
λ
Fig. 4-18
Interference stripes