START-UP
Axioscope 5/7/Vario
Mounting standard components
ZEISS
03/2018
430035-7344-001
55
3.1.10.6
Centering objectives of polarizing
microscope stands
The nosepiece with 5-positions HF/DF/Pol and
1-position HF/DF/DIC is equipped with five
centerable objective mounts (without DIC slots) as
well as one objective mount with DIC slot (non-
centerable). Accordingly, all objectives can be
centered relative to the rotary stage.
Stage centering is necessary to ensure that a
specimen feature located in the center of the field
of view does not move out while you are rotating
the stage. Centering all objectives ensures that the
specimen feature remains in the center of the field
of view even when the objective is changed.
•
Before centering the stage, you must adjust the
KÖHLER illumination on the microscope (see
section 4.2.1).
•
For stage centering, use a contrast-rich
specimen and an eyepiece with reticle.
•
First, turn the nosepiece to swing the non-centerable objective mount (mount with DIC slot) into the
light path. Center the rotary stage for the non-centerable objective mount as described
under 3.1.10.5.
•
Turn the nosepiece to move a centerable objective mount into the light path.
•
Rotate the stage to determine the position of maximum offset of the specimen feature (Fig. 3-18/
3
,
origin of arrow) from the center of the eyepiece reticle.
•
Turn the two centering screws on the nosepiece (Fig. 3-18/
1
) using one AF 1.5 Allen wrench
2
) each to move the specimen feature by half the arrow length towards the reticle center.
Rotate the stage again to check whether the specimen feature moves off. Repeat the centering
procedure, if necessary.
•
Center the other four objectives in the same manner.
NOTE
To preserve this centered state it is imperative to change the objectives only by turning the
nosepiece by its knurled ring, without touching the objectives.
Fig. 3-18
Centering objectives
