Zeiss Axioskop 40, Operating Manual

Page 1: ...Operating Manual Axioskop 40 Axioskop 40 FL Routine microscope...

Page 2: ...overed by an update service Unless expressly authorized forwarding and duplication of this document and the software package as well as utilization and communication of its contents are not permitted...

Page 3: ...ent safety 0 8 Notes on warranty 0 11 Overall view of Axioskop 40 0 12 Overall view of Axioskop 40 FL 0 13 CHAPTER 1 INSTRUMENT DESCRIPTION 1 2 1 1 Name and intended application 1 2 1 2 Instrument des...

Page 4: ...Axioskop 40 FL 2 17 2 2 Attachments and conversions 2 19 2 2 1 Changing the condenser 2 19 2 2 2 Inserting stops Ph Var and neutral filter in slider 2 20 2 2 3 Attaching filter mount 2 21 2 2 4 Attac...

Page 5: ...ation 3 29 3 5 6 Setting transmitted light polarization 3 30 3 5 7 Setting epi fluorescence 3 34 3 6 Documentation 3 36 3 6 1 Attaching photomicrography equipment 3 36 3 6 2 Attaching adapters for vid...

Page 6: ...SERVICE 4 2 4 1 Instrument care 4 2 4 2 Instrument maintenance 4 3 4 2 1 Performing checks 4 3 4 2 2 Changing fuses 4 3 4 3 Troubleshooting 4 4 4 4 Spares consumables and tools 4 8 4 5 Requesting serv...

Page 7: ...r that the specimen is not moved by the condenser If the 0 9 1 25 H condenser is equipped with the slider for PH H Var place it in the middle position for bright field 5 Close the luminous field diaph...

Page 8: ...equirements of EC directive 89 336 EC and EMC legislation dated September 18 1998 This operating manual includes information and warnings that must be observed by the user The following warning and in...

Page 9: ...ded against inadvertent operation Please contact a Zeiss service agency or the Carl Zeiss Microscopy Service to repair the instrument The power supply integrated in the stands of the microscopes enabl...

Page 10: ...etailed information please see Operating Manual B 40 065 e When fluorescence filters are used the heat protection filter for heat emitted by the microscope illuminator must not be removed since fluore...

Page 11: ...provided for defects caused by natural wear wearing parts and consumables in particular and improper use The instrument manufacturer is not liable for damage caused by faulty operation negligence or a...

Page 12: ...arl Zeiss Overall view of Axioskop 40 Axioskop 40 FL 0 12 B 40 810 e 12 01 Overall view of Axioskop 40 1 Eyepieces 2 Binocular tube 3 Stand 4 Transmitted light illuminator 5 Condenser 0 9 1 25 H 6 Mec...

Page 13: ...xioskop 40 FL Carl Zeiss B 40 810 e 12 01 0 13 Overall view of Axioskop 40 FL 1 Eyepieces 2 Ergo tube 20 23 with height adjustment 3 Stand 4 Incident light illuminator 5 Transmitted light illuminator...

Page 14: ...INTRODUCTION Axioskop 40 Carl Zeiss Axioskop 40 FL 0 14 B 40 810 e 12 01...

Page 15: ...IPTION Table of contents 1 INSTRUMENT DESCRIPTION 1 2 1 1 Name and intended application 1 2 1 2 Instrument description and main features 1 2 1 3 System Overview Axioskop 40 40 FL 1 4 1 4 Objectives 1...

Page 16: ...ergonomics operating convenience and function with technical performance Due to the modular design existing microscope modules from the Axioskop 2 plus and Axioplan 2 imaging such as tubes intermediat...

Page 17: ...for phase and variable contrast when used in combination with a separate slider for Ph Var Ball bearing 6 position nosepiece with W 0 8 thread ICS objectives in the cost performance categories A Plan...

Page 18: ...INSTRUMENT DESCRIPTION Axioskop 40 Carl Zeiss System overview Axioskop 40 40 FL Axioskop 40 FL 1 4 B 40 810 e 12 01 1 3 System Overview Axioskop 40 40 FL...

Page 19: ...Axioskop 40 INSTRUMENT DESCRIPTION Axioskop 40 FL System overview Axioskop 40 40 FL Carl Zeiss B 40 810 e 12 01 1 5...

Page 20: ...INSTRUMENT DESCRIPTION Axioskop 40 Carl Zeiss System overview Axioskop 40 40 FL Axioskop 40 FL 1 6 B 40 810 e 12 01...

Page 21: ...Axioskop 40 INSTRUMENT DESCRIPTION Axioskop 40 FL System overview Axioskop 40 40 FL Carl Zeiss B 40 810 e 12 01 1 7...

Page 22: ...ion factor 1 25x 2 5x 4x 5x 6 3x 10x 16x 20x 25x 32x 40x 50x 63x 100x 150x Objective magnification multiplied by eyepiece magnification usually 10x results in overall visual magnification e g 10 x 10...

Page 23: ...4 4 441031 0000 000 A Plan 20x 0 45 Ph 2 0 53 0 17 441041 0000 000 A Plan 40x 0 65 Ph 2 0 43 0 17 441051 0000 000 A Plan 100x 1 25 Oil Ph 3 0 22 0 17 441081 0000 000 VAREL contrast A Plan 10x 0 25 Ph...

Page 24: ...440760 0000 000 Plan Apochromat 100x 1 40 Oil 0 09 0 17 440780 0000 000 1 5 Eyepieces The following eyepieces are offered for the Axioskop 40 and Axioskop 40 FL Eyepiece Image angle Application Cat No...

Page 25: ...esignation technical data Cat No Stage micrometer positive 5 100 100 y D 0 17 mm gradation on the y axis 5 mm in 5 intervals gradation on the y axis 1 mm in 100 intervals with 2 opposing scales 10 m a...

Page 26: ...area 12 5x12 5 mm divided into 10x10 fields 474068 0000 000 Photo reticule MC 2 5x d 26 mm for 35 mm photography with 2 5x additional magnification or for large format photography with 10x additional...

Page 27: ...20 to 75 at 35 C Atmospheric pressure 800 hPa to 1060 hPa Operation Permissible ambient temperature 10 to 35 C Permissible relative humidity 20 to 75 at 35 C Atmospheric pressure 800 hPa to 1060 hPa...

Page 28: ...nment clean room Protection class I Protection type IP 20 Line voltage 100 VAC 240 VAC Line frequency 50 60 Hz Power consumption when HBO 103 is used 260 VA Fuses in accordance with IEC 127 Axioskop 4...

Page 29: ...stop can be set mechanically Achromatic aplanatic universal condenser 0 9 with swivel front lens 0 9 for objective magnifications Vobj 10 swivel out front lens 0 9 for objective magnifications Vobj 1...

Page 30: ...INSTRUMENT DESCRIPTION Axioskop 40 Carl Zeiss Axioskop 40 FL 1 16 B 40 810 e 12 01...

Page 31: ...luminator Axioskop 40 FL 2 17 2 2 Attachments and conversions 2 19 2 2 1 Changing the condenser 2 19 2 2 2 Inserting stops Ph Var and neutral filter in slider 2 20 2 2 3 Attaching filter mount 2 21 2...

Page 32: ...ot before rough adjustment 2 15 Fig 2 19 HBO 103 focal spot after rough adjustment 2 15 Fig 2 20 Focal spot image without adjustment guide on mechanical stage 2 16 Fig 2 21 Attaching HBO 50 2 17 Fig 2...

Page 33: ...2 43 Installing the filter and beam splitter 2 37 Fig 2 44 Changing the beam splitter 2 38 Fig 2 45 Changing the beam splitter 2 38 Fig 2 46 Beam splitter designation 2 39 Fig 2 47 Attaching the activ...

Page 34: ...ectives eyepieces condenser microscope mat and various small parts such as spare lamp dust cover and tool bag and other optional accessories are delivered in a separate package The following component...

Page 35: ...tion position required and tighten the hexagonal screw using the ball headed screwdriver CAUTION Do not hold the tube by the two eyepiece supports when moving it 2 1 3 Inserting eyepieces and auxiliar...

Page 36: ...new mount containing the eyepiece reticule required If you insert eyepiece reticules into the unscrewed mount take care to ensure that the labeling is visible the right way up after insertion Compensa...

Page 37: ...individual interpupillary distance by swinging the eyepiece tubes symmetrically toward one another Fig 2 5 The interpupillary distance is correct when you see only one round image when you look throu...

Page 38: ...s it unnecessary to constantly adjust the lamp voltage when switching objectives For magnifications up to 20x the 000000 1104 290 adapter ring is recommended it contains a neutral filter with 10 trans...

Page 39: ...8 1 Remove the refill set from the packaging The refill set consists of a marker insert 2 8 2 and a cover 2 8 5 Unscrew top 2 8 1 of the specimen marker If necessary remove used marker insert from the...

Page 40: ...re no longer visible Move condenser carrier 2 9 2 to the lowest position via drive for height adjustment 2 9 5 Insert condenser 2 9 1 between condenser carrier 2 9 2 and stage carrier 2 9 4 Orient the...

Page 41: ...e within this range The HBO 100 illuminator for epi fluorescence receives power from a separate transformer The HBO 100 W transformer must be connected to a power outlet via its power socket 2 11 2 on...

Page 42: ...ut tilting it Push new lamp 2 13 1 with its two socket pins carefully and without force into the lamp holder 2 13 2 Do not touch the front protective glass on the lamp The lamp must not be tilted othe...

Page 43: ...in Section 2 2 6 Changing the HBO 103 W 2 mercury vapor short arc lamp 2 Attaching the HBO 103 illuminator Remove cover from the incident light tube 2 14 1 Insert the dovetail of the lamp housing 2 14...

Page 44: ...0 W transformer 2 11 1 and allow it to warm up to the operating temperature Take care to ensure you do not look into the illuminator s light output opening Direct light beam against a projection surfa...

Page 45: ...djustment guide Use the knurled knob for collector adjustment 2 17 6 to focus on the brighter focal spot Use the SW 3 ball headed screwdriver on adjusting screw 2 17 4 to focus on the slightly darker...

Page 46: ...e on the white sheet of paper When required reduce the light intensity using attenuation filters in the 3 position or 6 position filter slider Use knurled knob 2 17 6 to focus on the brighter focal sp...

Page 47: ...ansformer The lamp will ignite automatically when switched on Adjusting the fluorescence illuminator Providing the Axioskop 40 FL is fitted with an adjustment guide to be ordered separately the HBO 50...

Page 48: ...lection so that both arcs appear the same size in the viewing window of the adjustment guide refer to Fig 2 22 6 Use the adjusting screws for height 2 22 2 and side adjustment 2 22 3 to center the arc...

Page 49: ...ring screws 2 23 3 until the condenser can be easily taken out of its mount Lift the condenser from the orientation groove by slightly tilting it upwards and pull it out to the front Use lever 2 23 8...

Page 50: ...ne insert each 2 24 9 into the ring gaps Hold the neutral filter 2 24 6 by the sides and insert into the middle of the slider Carefully place spring mount 2 24 7 over the neutral filter and press down...

Page 51: ...far as it will go Different filters 32 mm can be inserted in the filter mount e g the white balance filter If only one filter is to be used this can be placed directly on the luminous field diaphragm...

Page 52: ...er 2 27 7 into the front threaded hole to the left below the condenser carrier 2 27 3 as far as it will go Screw in stop bolt 2 27 2 with adjusting lever 2 27 1 as far as it will go into the rear thre...

Page 53: ...from the packaging and inserted into the HBO 103 illuminator if a protective mask and safety gloves are worn All electrical terminal connections must be made carefully Pronounced heat during operation...

Page 54: ...2 31 4 and remove the spent lamp and transport locking pin with the heat sink from holder 2 31 2 in the lamp holder 2 31 2 There is no need to remove the wire connection Undo fixing screw 2 31 5 on h...

Page 55: ...luminator mount 2 31 3 Take care to ensure that the cable to the heat sink is free at the side Release the spring lever Do not apply force to the lamp Fit the illuminator housing 2 30 1 onto the illum...

Page 56: ...at sink 2 32 2 with the burner 2 32 3 up out of the lamp holder 2 32 5 Put the heat sink down with the clamping screw accessible The cable on the heat sink should not be removed Undo the clamping scre...

Page 57: ...o lamp holder 2 32 5 Take care to ensure you only hold the heat sink Slowly let go of the spring lever and release the heat sink The heat sink must be aligned parallel to the lamp base To align it pre...

Page 58: ...changed as follows a X drive Push drive knob for x adjustment 2 33 4 all the way down Remove the enclosed adjustment pin 2 33 5 from the drive knob for y adjustment 2 33 1 and place it into one of th...

Page 59: ...manner that threaded holes 2 34 2 on the bottom of the stage are positioned above the stage carrier openings 2 34 3 Insert four fastening screws 2 34 5 through the stage carrier from below and screw...

Page 60: ...ring should be performed as follows Undo stage clamping screw 2 36 4 and the screw cap on the stage carrier 2 36 1 Rotate the stage to determine the maximum specimen deflection 2 36 5 tip of the arrow...

Page 61: ...the bottom additional sleeve 2 37 3 and remove the latter downwards then undo the two clamping screws 2 37 2 on the top additional sleeve 2 37 1 and pull it downwards as well Mount the additional slee...

Page 62: ...at regular intervals Make sure that the build up does not get into the guiding components for x adjustment 6 Changing the specimen holder on the mechanical stages 000000 1046 520 000000 1067 325 0000...

Page 63: ...39 3 and remove tube 2 39 1 upwards Place dust cap 2 39 2 for tube lens protection on the dovetail of the binocular tube Remove dust cap 2 39 5 from the desired tube Insert tube 2 39 1 with the dovet...

Page 64: ...he Axioskop connector plate 2 40 3 with the dovetail on the bottom into the stand opening 2 40 6 and slightly tighten clamping screw 2 40 5 Insert fastening screw 2 40 2 into the countersunk hole of t...

Page 65: ...mitted light only 000000 1046 282 Analyzer module D 000000 1050 958 Installing a module Pull the cover cap 2 41 1 forwards off the reflector turret Insert the module 2 41 3 with the retaining pins 2 4...

Page 66: ...tool set to unscrew retainer ring 2 42 1 Turn the reflector module around and allow the filter 2 42 2 or 5 to drop out on a soft surface The emission filter is inserted at 2 42 2 the excitation filter...

Page 67: ...ed to be installed so that the reflective layer 2 43 6 on the excitation filter 2 43 5 points outwards in relation to the reflector module With the emission filter 2 43 1 the reflective layer 2 43 2 p...

Page 68: ...wdriver Hold both halves of the reflector module together emission half 2 44 2 and excitation half 2 44 3 turn in the opposite direction to the installation position and put it down Tip up the excitat...

Page 69: ...the excitation half of the module 2 45 1 onto the emission half of the module 2 45 4 the retaining pins 2 45 5b will grip the eyelets 2 45 5a Hold both halves together and turn them back into the inst...

Page 70: ...2 47 9 match exactly Then tighten clamping screw 2 47 12 to prevent the co observation equipment from shifting Unscrew the tube lenses from the two binocular tubes using the cover of one of the storag...

Page 71: ...for 2 users 1 Ergonomic binocular phototube 2 Binocular tube 3 Dust cap 4 Rear dovetail 5 Socket for power unit 6 Heat protection shield 7 Fastening screw 8 Clamping screw 9 Support point stand 10 Pow...

Page 72: ...not forget to remove the protection caps from the tube ends Each pin must engage in the corresponding opening then tighten the coupling nuts Attach and clamp two binocular tubes for the multi observa...

Page 73: ...p to 9 observation stations to be set up with sufficient brightness for transmitted light bright field The weaker light phase and VAREL contrast procedures are also possible with slight restrictions 1...

Page 74: ...to the stand and tighten clamping screw 2 49 10 only slightly at first The dovetail is used to help centering Insert the second clamping screw with washer 2 49 3 into the drilled hole provided at the...

Page 75: ...ue illumination 3 29 3 5 6 Setting transmitted light polarization 3 30 3 5 7 Setting epi fluorescence 3 34 3 6 Documentation 3 36 3 6 1 Attaching photomicrography equipment 3 36 3 6 2 Attaching adapte...

Page 76: ...ing phase stop on the universal condenser 3 25 Fig 3 21 Centering phase stop light in the condenser to the phase ring dark in the objective 3 25 Fig 3 22 Condenser 0 9 1 25 H and slider with VAREL seg...

Page 77: ...Axioskop 40 OPERATION Axioskop 40 FL Carl Zeiss B 40 810 e 12 01 3 3...

Page 78: ...ioskop 40 Carl Zeiss Axioskop 40 operation and function controls Axioskop 40 FL 3 4 B 40 810 e 12 01 3 OPERATION 3 1 Axioskop 40 operation and function controls Fig 3 1 Axioskop 40 operation and funct...

Page 79: ...ametropia of the user s eyes to be compensated see Section 2 1 3 and also eyepiece reticules to be accepted see Section 1 6 Binocular tubes 3 1 2 The binocular tubes offered permit individual setting...

Page 80: ...hness Drive knob for movement of the mechanical stage in the x direction 3 1 9 Movement of the mechanical stage in the x direction maximum travel range 75 mm Adjustable torque smoothness Travel range...

Page 81: ...ight field position is also equipped with the enclosed neutral filter 25 transmission for compensating brightness differences between contrast procedures The phase and VAREL segment stops can be cente...

Page 82: ...cal stage 3 4 2 for placement positioning and fastening of specimens with the specimen holder 3 4 1 Nosepiece with objectives 3 1 15 Contains compartment 3 5 1 for compensators W 0 8 thread for six ob...

Page 83: ...r slip generates 3 dots Additional pressure on the ring triggers rotation of the ink pins 3 6 3 which generates 3 arcs The spring position of the ink pins prevents damage to the cover slip and writing...

Page 84: ...xioskop 40 Carl Zeiss Axioskop 40 FL operation and function controls Axioskop 40 FL 3 10 B 40 810 e 12 01 3 2 Axioskop 40 FL operation and function controls Fig 3 7 Axioskop 40 FL operation and functi...

Page 85: ...ction 15 Drive knob for mechanical stage adjustment in the x direction 16 3 levers to swivel filters in out in the filter magazine 17 Condenser centering screws either side 18 Universal condenser 19 M...

Page 86: ...rox 3 to 12 V On Off switch with control display 3 7 10 Position 0 Instrument turned off Position 1 Instrument turned on display glows green when the instrument is turned on Focusing drive rough adjus...

Page 87: ...ont to back 1 5 6 and 25 transmission lever in the front position filter swiveled out lever in the back position filter swiveled in Centering screw for condenser 3 7 17 Centering screws for condenser...

Page 88: ...3 9 1 Nosepiece with objectives 3 7 20 See page 3 8 Reflector turret 3 7 21 Accepts a maximum of five reflector modules such as the FL P C reflector module and shutter plate for incident light as well...

Page 89: ...7 23 Height adjustment of the condenser to set K HLER illumination Clamping lever for focusing drive height stop 3 7 24 Adjustable height stop for stage focusing Turn clamping lever up against the pi...

Page 90: ...st the height Adjustment can be determined by the centimeter interval scale on the side marked every cm 3 12 3 The ergo phototube has two adjustment positions Distribution 100 0 0 100 Push rod 3 12 1...

Page 91: ...ched camera via a push rod An upright unreversed image of the specimen appears in the binocular Push rod 3 13 2 pushed in 100 of the light to the eyepieces Push rod 3 13 2 pulled out 100 of the light...

Page 92: ...remaining in the beam path while the objective is being inserted Swivel overview adapter 3 15 2 into or out of the beam path with the handle 3 15 1 Take sure that the overview adapter engages correct...

Page 93: ...ercentage of the product of the individual transmission values Filter slider Axioskop 40 FL only The 3 position or 6 position FL filter slider permits 18 mm diameter filters e g PINKEL type filters to...

Page 94: ...trument on with the on off switch on the side of the instrument 3 17 1 The control display lights up Set the brightness desired using the light intensity regulator Switching off After you have finishe...

Page 95: ...ettings according to K HLER on the Axioskop 40 and Axioskop 40 FL 2 Transmitted light bright field equipment on the Axioskop 40 and Axioskop 40 FL Each Axioskop 40 microscope permits performance of th...

Page 96: ...3 19 5 to set the aperture diaphragm to approx 2 3 4 5 of the diameter of the objective exit pupils 3 18 E In most applications this aperture diaphragm setting provides optimum contrast at almost ide...

Page 97: ...f height stop 3 19 1 using an SW 3 ball headed screwdriver Use focusing drive to focus on the specimen Close luminous field diaphragm and focus via the height adjustment control 3 19 2 of the condense...

Page 98: ...shift The indirect light components diffracted at different cell components however bypass this optical channel and are influenced by the in phase refractive index and the thickness differences in the...

Page 99: ...vers 3 20 1 and 4 on the two centering screws 3 20 2 and 3 to recenter the bright phase stop until complete congruence with the dark phase ring is achieved 3 21 B With the 0 9 1 25 H condenser use the...

Page 100: ...ight dark field components for Axioskop 40 and Axioskop 40 FL 0 9 1 25 H condenser with slider equipped with VAREL segment stops Var 1 Var 2 Objectives suitable for VAREL segment stops A Plan 10x 0 25...

Page 101: ...ndenser if they feature an integrated aperture iris stop The Ph 3 stop of the PH H and Var slider can be used with the 0 9 1 25 H condenser and objectives 40x as a dark field stop 3 Setting transmitte...

Page 102: ...ous field diaphragm image will be almost perfectly in focus Finally match the diameter of the luminous field diaphragm to the size of the field of view Dark field microscopy requires specimens to be c...

Page 103: ...ft stop notches The procedure of oblique illumination would constitute a worsening in comparison with the VAREL contrast therefore the application is not recommended in connection with sliders equippe...

Page 104: ...birefringence thickness and orientation of the specimen These interference colors can be of the 1 st or a higher order 2 Axioskop 40 and Axioskop 40 FL configuration Objectives suitable for polarizat...

Page 105: ...n direction with the absolutely or relatively highest refractive index n and n direction with the absolutely or relatively lowest refractive index in relation to morphological directions e g of cryst...

Page 106: ...specimen e g an artificial fiber until the specimen displays maximum darkness The fiber is now parallel to one of the two directions of the crosslines If pronounced deviations 5 and more occur a pola...

Page 107: ...color chart 3 27 The surroundings of the artificial fiber which are not birefringent appear dark red when compensator is inserted which corresponds to the compensator s path difference of 550 nm 1 st...

Page 108: ...ves Plan Neofluar or Fluar UV excitation FL P C reflector module and shutter plate in the reflector turret HBO 103 or HBO 50 mercury vapor short arc lamp for incident light illumination Fluorescence p...

Page 109: ...unblocked Insert the eyepiece in the tube again and close luminous field diaphragm 3 28 5 until it is visible in the field of view Use the two centering screws 3 28 6 to center the luminous field dia...

Page 110: ...ve adapter rings with neutral filters should only be used for visual brightness compensation however not in conjunction with phototubes for photographic purposes Depending on film sensitivity using th...

Page 111: ...unit A into the phototube Align the camera unit in the required position and tighten with the three hexagonal screws 3 29 6 When artificial light color reversal film is used the color temperature key...

Page 112: ...on lens 456021 0000 000 3 30 4 into the microscope camera adapter 3 30 5 Attach MC 80 DX basic unit 3 30 2 to the microscope camera adapter as far as it will go align it and tighten clamping ring 3 30...

Page 113: ...T2 adapters for SLR camera housings Cat No T2 adapter for CONTAX CONTAX bayonet 416010 0000 000 T2 adapter for OLYMPUS OM OM bayonet 416002 0000 000 T2 adapter for MINOLTA SR bayonet 416003 0000 000...

Page 114: ...p color high resolution CCD cameras to be connected to the phototube of the Axioskop 40 40 FL Tube Adapters Cameras 456105 456105 0000 000 60 C 2 3 1 0 456107 000000 1069 414 60 C 2 3 0 63 456106 4561...

Page 115: ...e Screw the video adapter or video zoom adapter with C mount thread into the video camera Insert the video adapter or video zoom adapter into ENG 2 3 or ENG 1 2 bayonet of the video camera and clamp i...

Page 116: ...aching adapters for compact digital cameras Tube Adapters Cameras 000000 1096 522 D40 M52x0 75 To be used with 456006 0000 000 000000 1108 984 44 M52x0 75 To be used with 456140 0000 000 PHOTO TUBE AX...

Page 117: ...31 6 as far as it will go and tighten with the grub screw 3 31 5 Depending on the microscope configuration and the camera used the distance between the camera lens and the lens mount 3 31 4 might nee...

Page 118: ...d The drawing alongside shows the disassembled components Furthermore the M37 52 threaded ring adapter 3 32 3 can be unscrewed from the sliding mount 3 32 4 not shown so that cameras with M37 can also...

Page 119: ...tomatic Set the aperture as wide as possible i e select a small f stop number Not all cameras feature these options Please refer to the operating instructions of the camera used Undo grub screw 3 32 5...

Page 120: ...984 is designed especially for the Sony DSC S70 75 In conjunction with the Plan objectives e g Plan Neofluar photographs can be generated with increased edge clarity and decreased distortion Our mark...

Page 121: ...3 33 1 for the front tube mount of the microscope Binocular tube 30 23 3 33 2 or ergonomic binocular phototube 6 25 23 for the back tube mount of the co observation equipment The ergonomic binocular...

Page 122: ...uipment Axioskop 40 FL 3 48 B 40 810 e 12 01 1 Ergonomic binocular phototube 2 Binocular tube 3 Basic unit 4 Power unit 5 Adjusting knob for brightness control 6 Lever for light pointer Fig 3 33 Imple...

Page 123: ...ecommend that you have the multi observation equipment assembled by Carl Zeiss service staff since careful optical adjustment and centering of the fields of view of the various observation tubes is re...

Page 124: ...10 e 12 01 1 Adjusting knob for brightness control 7 Co observation tube 2 Basic unit 8 End connector L 3 Lever for light pointer 9 Power unit 4 Binocular tube 10 End connector R 5 Connecting piece L...

Page 125: ...de the microscope onto the intermediate image of the microscope This enables the user to copy microscope specimens by drawing Furthermore transparent references e g grain size plates reference scales...

Page 126: ...he specimen that corresponds to one interval of the crossline micrometer used For calibration align the scales of the stage micrometer and crossline micrometer parallel to each other by turning the ey...

Page 127: ...ter tenth estimated multiplied by the scale value k Example L 35 5 x 9 9 m 351 5 m Particularly large sample structures can also be determined by using the vernier scale gradations 0 1 mm on the mecha...

Page 128: ...OPERATION Axioskop 40 Carl Zeiss Axioskop 40 FL 3 54 B 40 810 e 12 01...

Page 129: ...INTENANCE TROUBLESHOOTING AND SERVICE Table of contents 4 CARE MAINTENANCE TROUBLESHOOTING AND SERVICE 4 2 4 1 Instrument care 4 2 4 2 Instrument maintenance 4 3 4 2 1 Performing checks 4 3 4 2 2 Chan...

Page 130: ...d immersion oil use a cotton swab or lint free cotton cloth and the optics cleaning solution L The cleaning solution comprises 90 Vol gasoline and 10 Vol isopropanol IPA The individual components are...

Page 131: ...by a qualified technician Ensure that the maximum operation time of the halogen and mercury vapor short arc lamps is not exceeded 4 2 2 Changing fuses Always pull the power plug before changing the f...

Page 132: ...ect position end position see Pages 3 16 3 17 The nosepiece with objective is not in click stop position Switch the nosepiece with objective to click stop position The condenser is not set correctly S...

Page 133: ...side should point up Use of no or non specified immersion oil with immersion objectives Use immersion oil 518 N from Carl Zeiss see P 4 8 Air bubbles in the immersion oil Apply new oil to remove the...

Page 134: ...ces to the appropriate ametropia s P 2 6 Eye fatiguing microscopy Interpupillary distance of the binocular tube is not set correctly Set the interpupillary distance correctly see P 2 7 The focusing ey...

Page 135: ...V 35 W halogen lamp is not used Use the specified 12 V 35 W halogen lamp see P 4 8 The fuses are defective Replace fuses see P 4 3 The electronic module is possibly defective Have electronic module c...

Page 136: ...44801 0000 00 recommended for low brightness techniques to suppress reflected light not so suitable for eyeglass wearers where necessary fold over eyecup Dust cover for nosepiece Dust cover for eyepie...

Page 137: ...Axioskop 40 FL may only be performed by Carl Zeiss service staff or specially authorized personnel To ensure optimum setting and trouble free function of your microscope over a longer period of time...

Page 138: ...CARE MAINTENANCE TROUBLESHOOTING AND SERVICE Axioskop 40 Carl Zeiss Axioskop 40 FL 4 10 B 40 810 e 12 01...

Page 139: ...xioskop 40 APPENDIX Axioskop 40 FL Overview Carl Zeiss B 40 810 e 12 01 A 1 APPENDIX List of abbreviations A 2 Index A 4 Certification in accordance with DIN ISO 9001 EN 46001 EC conformity declaratio...

Page 140: ...t d Difference of stage height setting focus difference in height measurement DC Direct Current DIC Differential Interference Contrast DIN Deutsches Institut f r Normung German Standards Institute DL...

Page 141: ...zed nM Refractive index of the medium nD Refractive index of D line sodium nP Refractive index of the specimen Ph Phase contrast PL Flat field R Right drive knob to the right of the mechanical stage S...

Page 142: ...efringence 3 30 Brightfield 3 13 3 21 3 27 Burner 2 26 C Centering screws for condenser 3 5 3 11 Changing fuses 4 3 Color temperature 3200 K 3 5 3 6 3 11 3 12 Condenser 2 10 2 19 2 21 3 5 3 6 3 7 3 11...

Page 143: ...p 3 5 3 9 3 11 3 15 Front lens 1 3 2 7 2 15 3 8 3 12 3 24 3 26 Front optics 2 10 2 19 H Halogen lamp 2 12 HBO 50 fluorescence illuminator 2 17 HBO 103 illuminator 2 13 Height stop on condenser carrier...

Page 144: ...pe camera 3 38 Multi observation equipment 2 42 3 47 3 49 N Nosepiece 2 8 3 5 3 8 3 11 3 14 3 22 O Objectives 1 8 2 8 2 30 Oblique illumination 3 29 On off switch 3 5 3 11 3 12 Operating the instrumen...

Page 145: ...itch on switch off 2 11 System overview 1 4 T Technical data 1 13 Tools 4 8 Torque setting 2 28 2 31 Transformer 2 11 Transmitted light 3 14 3 21 3 24 3 26 3 27 3 29 3 30 Transmitted light filter maga...

Page 146: ...APPENDIX Axioskop 40 Carl Zeiss Axioskop 40 FL A 8 B 40 810 e 12 01...