OPERATION
Axioskop 40
Carl Zeiss
Illumination and contrast procedures
Axioskop 40 FL
3-22
B 40-810 e 12/01
•
Swivel in 10x objective (yellow ring, also refer to
Chapter 1) into the nosepiece (3-18/
5
) and
focus on the specimen using the drive knob
(3-18/
2
).
•
Close luminous-field diaphragm (3-18/
4
) until it
becomes visible (even if not in focus) in the field
of view (3-18/
A
).
•
Use drive for height adjustment (3-19/
2
) to
lower the universal condenser until the edge of
the luminous-field diaphragm appears in focus
(3-18/
B
).
•
Use both centering screws (3-18/
3
) to center
the luminous-field diaphragm image (3-18/
C
)
and then open the diaphragm until its edge just
disappears from the field of view (3-18/
D
).
•
For aperture diaphragm setting (contrast),
remove one eyepiece from the tube and look
through the tube with your naked eye. Use
adjusting lever (3-19/
5
) to set the aperture
diaphragm to approx. 2/3
...
4/5 of the
diameter of the objective exit pupils (3-18/
E
). In
most applications, this aperture diaphragm
setting provides optimum contrast at almost
ideal resolution, and is therefore the best
compromise for the human eye.
•
Insert eyepiece back in the tube.
☞
Since specimen field size and objective aperture change after every objective change as does
the centering slightly under some circumstances, the settings of the luminous-field diaphragm
and aperture diaphragm must be repeated to obtain optimum results.
For < 10x objectives, the front lens of the universal condenser (if used) must be folded out and
the aperture diaphragm fully opened. In the case of such large fields, the luminous-field
diaphragm can also be used for better contrasting by its opening being reduced by a certain
range. Closing it too much should be avoided in order not to worsen the evenness of the field
of view illumination.
Fig. 3-18
Axioskop 40 microscope settings for
transmitted-light bright field
Summary of Contents for Axioskop 40
Page 1: ...Operating Manual Axioskop 40 Axioskop 40 FL Routine microscope...
Page 14: ...INTRODUCTION Axioskop 40 Carl Zeiss Axioskop 40 FL 0 14 B 40 810 e 12 01...
Page 30: ...INSTRUMENT DESCRIPTION Axioskop 40 Carl Zeiss Axioskop 40 FL 1 16 B 40 810 e 12 01...
Page 77: ...Axioskop 40 OPERATION Axioskop 40 FL Carl Zeiss B 40 810 e 12 01 3 3...
Page 128: ...OPERATION Axioskop 40 Carl Zeiss Axioskop 40 FL 3 54 B 40 810 e 12 01...
Page 146: ...APPENDIX Axioskop 40 Carl Zeiss Axioskop 40 FL A 8 B 40 810 e 12 01...
