Zeiss Axiostar plus, Operating Manual

Page 1: ...t Service Center Repairs Experienced Engineers and Technicians on staff in our State of the art Full Service In House Service Center Facility InstraView Remote Inspection Remotely inspect equipment be...

Page 2: ...Operating Manual Axiostar plus Transmitted Light Microscope Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com...

Page 3: ...sly authorized forwarding and duplication of this document and the utilization and communication of its contents are not permitted Violations will entail an obligation to pay compensation All rights r...

Page 4: ...rgonomic base plate 2 3 2 2 1 Attaching the ergonomic base plate 2 4 2 2 2 Adjusting the ergonomic base plate 2 6 2 3 Attaching tubes 2 8 2 3 1 Attaching binocular tube 45 20 ICS 2 8 2 3 2 Attaching b...

Page 5: ...3 2 Using the transmitted light brightfield technique 3 4 3 2 1 General principle 3 4 3 2 2 Transmitted light brightfield configuration 3 4 3 2 3 Setting transmitted light brightfield for K HLER illu...

Page 6: ...with other filter thread sizes 3 26 3 11 4 Sony DSC S70 DC S75 DSC S85 on digital camera adapter 44 M52x0 75 000000 1108 984 3 26 3 12 Axiostar plus Packing the mount 3 27 4 CARE MAINTENANCE AND TROUB...

Page 7: ...e text by the following symbols CAUTION If the safety notes are not observed there is a risk for the user CAUTION Disconnect the instrument from the line CAUTION Hot surface there is a risk of burns C...

Page 8: ...Please observe the following safety notes when using the Axiostar plus microscope The microscope can be operated with a line voltage of 100 240 V The microscope must be connected only to a properly in...

Page 9: ...ingerprints Notes on Warranty The Axiostar plus microscope including its original accessories may only be used for the microscope techniques described in this manual The manufacturer cannot assume any...

Page 10: ...1 3 1 1 Name and intended application 1 3 1 2 Description and main features 1 3 1 3 System overview 1 4 1 4 Objectives 1 7 1 5 Eyepieces 1 9 1 6 Stage micrometers and eyepiece reticles 1 9 1 7 Technic...

Page 11: ...Instrument description Carl Zeiss Overall view Axiostar plus 1 2 B 40 815 e 12 01 Fig 1 1 Overall view Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com...

Page 12: ...ht microscope In addition to high resolution ICS objectives and the major brightfield darkfield phase contrast polarization contrast and epi fluorescence techniques an optional camera port for photo a...

Page 13: ...Instrument description Carl Zeiss System overview Axiostar plus 1 4 B 40 815 e 12 01 1 3 System overview Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com...

Page 14: ...Instrument description Axiostar plus System overview Carl Zeiss 40 815 e 12 01 1 5 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com...

Page 15: ...Instrument description Carl Zeiss System overview Axiostar plus 1 6 B 40 815 e 12 01 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com...

Page 16: ...g indicates that these objectives may only be used with microscopes featuring infinite optics ICS and not with instruments whose objectives are marked with 160 as their mechanical tube length in mm Th...

Page 17: ...0000 000 A Plan 100 1 25 Oil 0 22 0 17 441080 0000 000 Phase contrast A Plan 10 0 25 Ph 1 4 4 441031 0000 000 A Plan 20 0 45 Ph 2 0 53 0 17 441041 0000 000 A Plan 40 0 65 Ph 2 0 43 0 17 441051 0000 0...

Page 18: ...der Cat No 444801 0000 000 1 6 Stage micrometers and eyepiece reticles Measuring and counting using the microscope requires stage micrometers and eyepiece reticles a small selection of which is listed...

Page 19: ...or 10 10 474068 0000 000 Photo reticle MC 2 5 d 26 mm for 35mm photography with an additional magnification of 2 5 or for large format photography with a 10 additional magnification 454075 0000 000 F...

Page 20: ...35 C Permissible relative humidity without condensation max 85 Air pressure 800 hPa to 1060 hPa 4 Operating data Category of use closed rooms Protection class I Protection type IP 20 Electrical safet...

Page 21: ...45 mA Average life with 9 V alkaline battery depending on battery capacity Maximum intensity approx 6 5 h 65 of maximum intensity approx 16 h 50 of maximum intensity approx 21 h Operation with 12 V ba...

Page 22: ...nier readable from the right Specimen holder with spring clip to the left Abbe condenser 0 9 1 25 for VObj 4 with wide angle function 445312 0000 000 Binocular tube 45 20 maximum field number 20 inter...

Page 23: ...25 535 545 555 mm visual port tube factor 1x Binocular phototube 30 20 maximum field number 20 interpupillary distance can be set between 55 and 75 mm viewing angle 30 viewing angle with ergonomic bas...

Page 24: ...ng drive knob torque for x y adjustment on the mechanical stage 2 13 2 8 Attaching condensers 2 14 2 8 1 Attaching Abbe condenser 0 9 1 25 2 14 2 8 2 Inserting slider for phase contrast or darkfield 2...

Page 25: ...2 15 Attaching LED illuminator 2 16 Fig 2 16 Connecting LED illuminator to power source 2 17 Fig 2 17 Attaching darkfield condenser 2 18 Fig 2 18 Attaching colored disk carrier polarizer or wide angle...

Page 26: ...on the work table Keep the packaging so that the instrument can be stored for a longer period of time or returned to the manufacturer 2 2 Attaching and setting ergonomic base plate Using the Axiostar...

Page 27: ...esive or varnish remains from the drilled holes Turn over the stand and place it top side down on a soft surface or the packing cardboard Fig 2 2 Secure the microscope in this position or have a secon...

Page 28: ...usting ergonomic base plate Carl Zeiss B 40 815 e 12 01 2 5 1 Ergonomic base plate 2 Allen screws M4 x 30 Fig 2 2 Attaching ergonomic base plate Artisan Scientific Quality Instrumentation Guaranteed 8...

Page 29: ...handle on the back of the ergonomic base plate 2 3 2 The adjustment feet 2 3 3 will disengage and can be adjusted Select desired height by turning the adjustment feet then lower the entire unit The a...

Page 30: ...plate Carl Zeiss B 40 815 e 12 01 2 7 1 Top of stand 2 Handle on back of ergonomic base plate 3 Adjustment feet 4 Locking button Fig 2 3 Adjusting ergonomic base plate Artisan Scientific Quality Instr...

Page 31: ...e hexagonal screw 2 4 4 from the stand Press the binocular tube 2 4 2 in a slightly inclined position to the right into the spring of the stand Then set the tube in the dovetail of the stand by loweri...

Page 32: ...the right into the spring of the stand Then set the adapter in the dovetail of the stand by lowering the left side This secures the adapter from falling out Turn the adapter to align the outer edges p...

Page 33: ...4 or 5 position nosepiece 2 Dust cap 3 Objective Fig 2 6 Screwing in objectives 1 Focusing eyepiece 2 Fixed eyepiece 3 Binocular tube 4 Dust caps Fig 2 7 Inserting eyepieces 2 5 Inserting eyepieces R...

Page 34: ...iece reticle from the eyepiece Screw in the new mount containing the required eyepiece reticle 2 5 2 Compensation of ametropia when eyepiece reticles are used The correct use of an eyepiece reticle re...

Page 35: ...n rings fit very tightly in the eyepiece groove so that a dull object e g tooth pick is required to press them out 2 6 Setting interpupillary distance and viewing height The eyepiece distance is match...

Page 36: ...llows X drive Move the drive knob for x adjustment 2 11 4 all the way down Remove the adjustment pin 2 11 5 from the y adjustment drive knob 2 11 1 and place into the bottom nut 2 11 3 Hold drive knob...

Page 37: ...erted into the condenser carrier Insert the condenser into the condenser carrier in such a way that 1 the dovetail of the condenser is pressed against the spring pin 2 12 2 in the condenser carrier an...

Page 38: ...carrier Make sure that the orientation screw 2 14 5 on the condenser engages in the groove 2 14 4 of the condenser carrier Tighten both centering screws 2 14 3 on the condenser carrier 2 14 1 until t...

Page 39: ...ost position Power to the LED illuminator can be supplied either via the line power unit or independent of the line via a commercially available 9 V Alkaline battery 9 V Ni Cd battery block in the int...

Page 40: ...s as long as there is sufficient loading capacity from the battery If the loading capacity is exhausted the LED illuminator will go out or drop to minimum brightness After connection to the voltage so...

Page 41: ...ace darkfield condenser 2 17 6 in the darkfield condenser frame 2 17 5 and screw on fastening ring 2 17 7 The dry darkfield condenser 0 7 0 85 465506 000 000 is screwed directly into the darkfield con...

Page 42: ...40 X 0 65 B Achroplan 440040 0000 000 20 X 0 45 B C Achroplan 440068 0000 000 63 X 0 95 A Achroplan 440060 0000 000 63 X 0 80 A Achrostigmat 440286 0000 000 100 X 1 25 A CP Achromat 440953 9901 000 50...

Page 43: ...r 2 18 6 into the front thread on the bottom left of the condenser carrier 2 18 1 position a using the 90 offset hexagonal key SW 2 2 18 5 until it engages Screw stop bolt 2 18 3 with adjusting lever...

Page 44: ...out push the filter clamp 2 19 1 in the groove 2 19 3 until it engages A maximum of two filters can be secured with the filter clamp There are depressions 2 19 5 on the top of the luminous field diaph...

Page 45: ...rews 2 20 5 Press epi fluorescence illuminator 2 20 2 at a slight angle to the right into the spring of the stand mount Then press the epi fluorescence illuminator into the dovetail mount of the stand...

Page 46: ...Filter stop carrier 2 designated with two dots also has three positions Position 1 adjust is used to adjust the fluorescent lamp see section 2 11 4 Position 2 open is a free passage but a 25 mm diamet...

Page 47: ...p 2 22 1 on the connection supports on the epi fluorescence illuminator 2 22 3 align and tighten with clamping screw 2 22 2 Plug the HBO 50 connection cable into the power supply and plug the power su...

Page 48: ...key SW 3 and lift up lamp housing Press spring handle down 2 23 4 and lift up heat sink 2 23 2 with the burner 2 23 3 out of the lamp frame 2 23 5 Set it down so that clamping screw on the heat sink...

Page 49: ...heat sink Avoid getting fingerprints on the glass components of the burner If fingerprints do occur remove immediately Press down spring handle 2 23 4 and place burner with heat sink into the lamp fra...

Page 50: ...the collector so both light arcs are sharply focused Use adjustment screw 2 24 1 to adjust burner in an axial direction to the mirror so that both light arcs appear the same size in the adjustment gu...

Page 51: ...tive cap 2 25 2 on the epi fluorescence illuminator 2 25 1 along the groove Push the reflector carriage lever 2 25 5 to the right Turn lock 2 25 3 to horizontal position open and pull out the reflecto...

Page 52: ...2 25 4 to the correct insertion position and place in the epi fluorescence illuminator Hold the lever 2 25 5 to the right and push in the reflector carriage until it engages Turn lock 2 25 3 clockwise...

Page 53: ...he locking filter emission filter 2 27 2 and excitation filter 2 27 5 and secure with the fastening 2 27 1 The emission filter and excitation filter will be labeled with an inscription or arrow on the...

Page 54: ...rdering completely equipped P C reflector modules since changing the color splitter has higher requirements To replace a color splitter Remove P C reflector module from reflector revolver Loosen both...

Page 55: ...splitter and carefully place the new one on the spring frame 2 30 3 with the reflective side pointing up Place both parts together in the bottom module half Make sure that the side catch of the spring...

Page 56: ...contact plug 2 32 3 to the line Switch on the instrument via the on off switch 2 32 4 on the left hand side of the instrument The green LED integrated into the on off switch lights up to indicate that...

Page 57: ...Start Up Carl Zeiss Axiostar plus 2 34 B 40 815 e 12 01 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com...

Page 58: ...2 3 5 3 Setting transmitted light polarization contrast 3 12 3 5 4 Sample differentiation between gout and pseudogout 3 13 3 6 Setting epi fluorescence 3 14 3 6 1 General principle 3 14 3 6 2 Epi fluo...

Page 59: ...disk 3 7 Fig 3 7 Centering phase stop 3 7 Fig 3 8 Setting transmitted light darkfield 3 9 Fig 3 9 Setting transmitted light polarization contrast 3 12 Fig 3 10 Setting epi fluorescence 3 15 Fig 3 11...

Page 60: ...otography of stained sections and for phase contrast CB 3 conversion filter d 32 x 2 to generate the correct color temperature of 3200 K when artificial light color reversal film is used CB 3 d 32 x 2...

Page 61: ...nfigured to permit the transmitted light brightfield technique When using the Abbe condenser with 5 position turret set the brightfield position H via the turret disk If a slider for phase contrast is...

Page 62: ...contrast remove one eyepiece from the tube and look into the tube with your naked eye Use lever 3 4 3 to set the aperture diaphragm to approx 2 3 4 5 of the diameter of the objective exit pupil 3 4 E...

Page 63: ...he small phase differences in intensity and color differences which are visible to the human eye Optimum phase contrast requires a high level of cleanliness Therefore clean the front lens of the objec...

Page 64: ...ive e g Ph 2 or set the relevant position on the turret disk 3 6 2 of the Abbe condenser with 5 position turret Adjust the brightness Check whether phase stop centering complies with the figure 3 7 Fo...

Page 65: ...ight on a dark background although they partially lie below the resolving power of the light microscope 3 4 2 Transmitted light darkfield configuration Condenser with slider for darkfield When using t...

Page 66: ...htness distribution For darkfield setting it is also possible to use the slider for phase contrast 3 and the following objectives instead of the slider for darkfield 0 25 0 9 CP Achromat A Plan and Pl...

Page 67: ...This will make the imaging of the luminous field diaphragm easier to identify Close luminous field diaphragm by turning luminous field diaphragm ring counterclockwise until it makes contact Lower the...

Page 68: ...he specimen location swivel in immersion oil objective and focus specimen Close luminous field diaphragm by turning luminous field diaphragm ring counterclockwise until it makes contact Lower the cond...

Page 69: ...ect interference colors will occur from gray usually in biological objects to white yellow red and blue These interference colors can be of 1 st or higher order 3 5 2 Transmitted light polarization co...

Page 70: ...set the oscillation direction to 45 stop position Select crystal needles that are oriented in the gamma direction see marking on the lambda plate Analysis If the crystal needles oriented parallel to...

Page 71: ...r sets and fluorochromes available from Zeiss at www zeiss de micro Fluorescent microscopy under Techniques of Microscopy 3 6 2 Epi fluorescence configuration Recommended objectives Plan Neofluar or F...

Page 72: ...er combination on the reflector carrier into the beam path Turn off the halogen lamp on microscope and open illumination beam path Move the filter stop carrier 2 to the open position Refocus on the sp...

Page 73: ...f the used eyepiece crossline micrometer For calibration align the scales of the stage micrometer and the crossline micrometer parallel to each other by turning the eyepiece and make the zero lines of...

Page 74: ...m the front Remove dust caps from the eyepiece tubes and insert two eyepieces each 3 12 2 with the same field number 18 or 20 Insert country specific adapter 3 12 9 into the power unit 3 12 8 Insert a...

Page 75: ...ras e g MC 80 DX to be attached to the camera port of the Axiostar plus If focusing is not to be made via the viewfinder of the camera the component with the eyepiece reticle must be screwed in the ey...

Page 76: ...ilter Various T2 adapters for SLR cameras are listed below T2 adapters for SLR camera housings Cat No T2 adapter for CONTAX CONTAX bayonet 416010 0000 000 T2 adapter for OLYMPUS OM OM bayonet 416002 0...

Page 77: ...fix it by turning clamping ring 3 14 3 anti clockwise Attach 35 mm Mot DX film cassette 3 14 1 to the basic body in such a way that the contact pins firmly engage in the relevant sockets For micropho...

Page 78: ...00 1069 414 60 C 2 3 0 63 456106 456106 0000 000 60 C 1 2 0 5 456108 456108 0000 000 60 C 1 3 0 4 452989 0000 000 Zoom 44 C 1 3 3 CCD 0 35x 1 6x To be used with 456140 0000 000 456115 456115 0000 000...

Page 79: ...oom adapter with C mount thread into the video camera Insert video adapter or video zoom adapter in ENG 2 3 or ENG 1 2 bayonet of the video camera and clamp it tight Insert premounted unit video camer...

Page 80: ...eras Tube Adapter Cameras 000000 1096 522 D40 M52x0 75 To be used with 456006 0000 000 000000 1108 984 44 M52x0 75 To be used with 456140 0000 000 AXIOSTAR PLUS PHOTO TUBE WITH INTERFACE 60 MM COMPACT...

Page 81: ...hread of the camera 3 15 1 With the lens mount 3 15 4 pointing forward plug the unit onto connector 60 3 15 6 until stop and tighten the set screw 3 15 5 Depending on the microscope configuration and...

Page 82: ...16 4 not shown i e cameras with M37 can also be attached as described in the previous chapter Screw SONY VAD S70 adapter ring 3 16 2 into the objective filter thread of the DSC S70 camera 3 16 1 unti...

Page 83: ...n a camera adapter combination is used which is not recommended by Carl Zeiss 3 11 3 Attaching cameras with other filter thread sizes In principle it is also possible to connect cameras with a filter...

Page 84: ...se floor 3 17 2 Insert microscope into the recess in the case floor Push rubber foam component 3 17 4 over the condenser 3 17 5 and move the condenser upwards until it contacts the rubber foam compone...

Page 85: ...r 2 Case floor 3 Velcro fasteners 4 Rubber foam component 5 Condenser 6 Binocular tube 45 ICS 7 Rubber foam component 8 Holding flaps 9 Stand Fig 3 17 Inserting the Axiostar plus in the case Artisan S...

Page 86: ...aintenance of the instrument 4 2 4 2 Troubleshooting 4 3 4 2 1 Changing the fuses 4 3 4 2 2 Changing the 6 V 20 W halogen lamp 4 4 4 2 3 Troubleshooting 4 5 4 2 4 Spares wearing parts and tools 4 8 4...

Page 87: ...cs cleaning mixture L This cleaning mixture is produced of 90 Vol benzoline and 10 Vol isopropanol IPA The various components are also known under the following synonyms benzoline medical alcohol petr...

Page 88: ...socket Check line cable and plugs and exchange them if required Use a 2 0 mm clockmaker s screwdriver to press in the two spring shackles on the sides of the fuse holder over the slot and remove the f...

Page 89: ...witch and allow lamp to cool down for approx 15 minutes Disconnect the earth contact plug from the line and remove the flat plug from the instrument connector Place the disconnected instrument on its...

Page 90: ...t Insert filter correctly in filter mount see p 3 3 Low resolving power and poor image contrast Aperture diaphragm opening not set correctly Set aperture diaphragm opening in accordance with the 2 3 r...

Page 91: ...yepieces are not set correctly Set focusing eyepieces to the appropriate ametropia see p 2 11 and 2 12 Eye fatiguing microscopy Interpupillary distance of the binocular tube is not set correctly Set c...

Page 92: ...lamp is not used Use the specified 6 V 20 W halogen lamp see p 4 8 Defective fuses Exchange fuses see p 4 3 Electronics module possibly defective Have electronics module checked by microscopy service...

Page 93: ...Interference band filter green 546 d 32 3 CB 12 conversion filter d 32 2 CB 6 conversion filter d 32 2 CB 3 conversion filter d 32 2 3200 5500 K conversion filter d 32 2 N 0 25 neutral density filter...

Page 94: ...ion of your microscope over a longer period of time we would recommend that you enter into a service maintenance contract with Carl Zeiss In the case of subsequent orders or when service is required p...

Page 95: ...Care maintenance and trouble shooting Carl Zeiss Axiostar plus 4 10 B 40 815 e 12 01 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com...

Page 96: ...ENDIX Index A 2 List of Abbreviations A 4 Physical and Technical Units A 5 Certification in Accordance with DIN EN ISO 9001 DIN EN 46001 EC Conformity Declaration Artisan Scientific Quality Instrument...

Page 97: ...slip thickness 1 7 1 8 D Dark field slider 3 9 Darkfield condenser 2 18 2 19 3 9 Digital compact cameras 3 23 Dimensions 1 11 Diopter 3 6 3 7 Disconnect VII VIII Disposal VII Double tube 3 17 Dust co...

Page 98: ...Packing 3 27 Phase contrast 3 7 Phase contrast objective 3 6 Polarizer 3 12 Power unit 3 17 S Service 4 9 Slider for darkfield 3 8 Slider for phase contrast 2 14 3 4 3 6 Slider for phase darkfield 2 1...

Page 99: ...rmation e g film speed EG European Community EMV electromagnetic compatibility EN European standards ENG Electronic News Gathering E PL name of eyepiece type with aspheric lens and flat field of view...

Page 100: ...Obj magnification of the objective VDE association of German electrotechnicians vis visual W 0 8 Whitworth type thread inch thread 0 8 Physical and Technical Units A ampere angular degree C Centigrade...

Page 101: ...Appendix Carl Zeiss Axiostar plus A 6 B 40 815 e 12 01 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com...

Page 102: ...t Service Center Repairs Experienced Engineers and Technicians on staff in our State of the art Full Service In House Service Center Facility InstraView Remote Inspection Remotely inspect equipment be...