Carl Zeiss
Axiotech and Axiotech
vario
3-12
B 40-020 e 06/99
3.4.3
Setting of reflected-light interference contrast
Differential interference contrast (DIC) is based on
polarization contrast and is ideal for
the
visualization of minute elevation differences in
surfaces. A birefringent prism ( 3-11/
4
) is used to
split the polarized illumination beams in two
partial beams with slight lateral displacement.
These two beams hit the sample surface ( 3-11/
6
)
with lateral displacement from each other and
pass slightly different optical paths in accordance
with the
∆
h differential elevation differences. The
partial beams now assigned with a path difference
of 2
∆
h then pass the objective ( 3-11/
5
) and the
birefringent prism
( 3-11/
4
) in the opposite
direction and can interfere with each other after
passing the analyzer ( 3-11/
7
) because they now
feature the same vibration direction again. In the
intermediate image, the path difference assigned
on the sample surface ( 3-11/
6
) changes into gray
values which can be seen by the eye. Steps
become visible as a relief. An additionally inserted
lambda plate ( 3-11/
7a
) changes the gray values
into colors again.
The Axiotech and Axiotech
vario
allow differential interference contrast to be performed with all objectives
with magnification
≥
5
×
.
•
Perform steps described in sections 3.2 and 3.3.
☞
The DIC technique with reflector slider HD DIC ( 3-12/
3
) can be performed in two different
ways:
•
Version 1:
DIC prism slider (3-12/
5
) with fine adjustment for Epiplan objectives and with
DIC compensator (
λ
), Cat. No. 444410-0000-000
•
Version 2:
DIC compensation prism slider (3-12/
4
) for Epiplan, Epiplan-Apochromat and
Epiplan-Neofluar objectives, Cat. No. 444411-0000-000.
Fig. 3-11
Reflected-light interference contrast
Summary of Contents for Axiotech
Page 1: ...Axiotech and Axiotechvario Materials Microscopes Operating Manual...
Page 14: ...Carl Zeiss Axiotech and Axiotechvario XIV B 40 020 e 06 99...
Page 16: ...Carl Zeiss Axiotech and Axiotechvario 1 2 B 40 020 e 06 99...
Page 112: ...Carl Zeiss Axiotech and Axiotechvario 3 46 B 40 020 e 06 99...
Page 114: ...Carl Zeiss Axiotech and Axiotechvario 4 2 B 40 020 e 06 99...
Page 124: ...Carl Zeiss Axiotech and Axiotechvario A 2 B 40 020 e 06 99...
