Axiotech and Axiotech
vario
Carl Zeiss
B 40-020 e 06/99
3-15
3.4.4
Setting of reflected-light polariza-
tion contrast
Reflected-light polarization contrast is the ideal
technique for surface structures which change the
state of polarization during reflection.
The
illumination light ( 3-13/
1
) is linearly polarized by
the polarizer
( 3-13/
2
) and, after passing the
objective (3-13/
4
), hits the sample surface ( 3-13/
5
)
where it is reflected. Here, the beam portions
experience structure-dependent path differences
and polarization-optical rotations which are
visualized as different gray values after the
analyzer (3-13/
6
) has been passed. A compensator
with lambda plate (3-13/
6a
) enables the change
from gray contrast to color contrast.
When objectives with a very low magnification are
used, a rotary
λ
/4 plate (Antiflex cap) in front of
the objective permits otherwise unavoidable
reflection to be also eliminated from
"dark"
sample surfaces.
•
Perform steps described in sections 3.2 and 3.3.
☞
No filter should be inserted in the
beam path for intensity reasons.
•
Do not leave the DIC prism slider (3-14/
5
) in any
function position, i.e. either push it in until the
first stop or remove it completely and replace it
with the protection cap (3-12/
6
).
•
If required, remove filter slider ( 3-14/
1
) with
filters or switch to free light path.
•
Push HD DIC reflector slider ( 3-14/
3
) into the
reflected-light illuminator until the DIC position
is active (
DIC
mark visible on the reflector
slider ).
Fig. 3-13
Reflected-light polarization contrast
Fig. 3-14
Setting of reflected-light
polarization contrast
Summary of Contents for Axiotech
Page 1: ...Axiotech and Axiotechvario Materials Microscopes Operating Manual...
Page 14: ...Carl Zeiss Axiotech and Axiotechvario XIV B 40 020 e 06 99...
Page 16: ...Carl Zeiss Axiotech and Axiotechvario 1 2 B 40 020 e 06 99...
Page 112: ...Carl Zeiss Axiotech and Axiotechvario 3 46 B 40 020 e 06 99...
Page 114: ...Carl Zeiss Axiotech and Axiotechvario 4 2 B 40 020 e 06 99...
Page 124: ...Carl Zeiss Axiotech and Axiotechvario A 2 B 40 020 e 06 99...
