Carl Zeiss
Axiotech and Axiotech
vario
3-30
B 40-020 e 06/99
•
To check the centering and congruence of the bright phase stop (in the condenser) with the dark
phase ring (in the objective), remove one eyepiece from the tube and replace it with the centering
telescope. Use the correction facility of the centering telescope to focus on the phase stop and phase
ring in the objective exit pupil. Open the aperture diaphragm completely.
•
If congruence is not perfect ( 3-28/
A
), the two
knurled screws (3-27/
2
) on the
ABBE
condenser
must be used to recenter the bright phase stop
until complete congruence with the dark phase
ring has been achieved (3-28/
B
). Tighten
fixation screw (3-27/
3
) to retain this setting.
•
Finally, remove the centering telescope from
the tube and replace it with the eyepiece.
☞
To enhance the image contrast, an interference wide-band filter, green 32
×
4, can be placed
on the luminous-field diaphragm.
Complete phase contrast is only achieved if the bright phase stop (in the condenser) and the
dark phase ring (in the objective) are exactly congruent in the illumination beam path (3-28/
B
).
Even more than brightfield, phase contrast requires particularly clean glass-to-air surfaces on
the specimen and the optics. Make sure to avoid finger prints in particular.
3.4.10
3D Microscopy
Detailed information on 3D microscopy using the Axiotech 100 and Axiotech
vario
100 is contained in the
following operating manuals (B) and brief instructions (K) from Carl Zeiss;
Axiotech 100:
B 40-022-2,
K 40-022-2
Axiotech
vario
100:
B 40-022-3,
K 40-022-3
In its center position, the reflector slider 2 FL/3D (forensic) contains a 1:1 beam splitter for the visual
range, and a depolarizer for 3D microscopy in the eyepiece version. The two outer positions can be
equipped as required with filters and beam splitters for fluorescence applications.
Fig. 3-28
Centering of phase stop (bright in
condenser) with phase ring (dark in
objective)
Summary of Contents for Axiotech
Page 1: ...Axiotech and Axiotechvario Materials Microscopes Operating Manual...
Page 14: ...Carl Zeiss Axiotech and Axiotechvario XIV B 40 020 e 06 99...
Page 16: ...Carl Zeiss Axiotech and Axiotechvario 1 2 B 40 020 e 06 99...
Page 112: ...Carl Zeiss Axiotech and Axiotechvario 3 46 B 40 020 e 06 99...
Page 114: ...Carl Zeiss Axiotech and Axiotechvario 4 2 B 40 020 e 06 99...
Page 124: ...Carl Zeiss Axiotech and Axiotechvario A 2 B 40 020 e 06 99...
