Zeiss Axiovert 100, Operating Manual

Page 1: ...3D Microscopy for Axiovert 100 135 135 M Operating Manual ...

Page 2: ...are subject to change Unless expressly authorized forwarding and duplication of this document and the utilization and communication of its contents are not permitted Violations will entail an obligation to pay compensation All rights reserved in the event of granting of patents or registration of a utility model Issued by Carl Zeiss Mikroskopie D 07740 Jena Telephone 03641 64 16 16 Telefax 03641 6...

Page 3: ... of eyepiece and monitor version 10 2 3 Maintenance and care 11 2 3 1 General 11 2 3 2 Cleaning of optical components 11 2 3 3 Change of voltage and fuses 11 2 3 4 Troubleshooting 12 3 Technical Data 13 Certification in accordance with DIN EN ISO 9001 and DIN EN 46001 EC Declaration of Conformity Annex optional 3D microscopy for Axiotron 2 3D microscopy for Axiotech 100 3D microscopy for Axiotech ...

Page 4: ... operation and function of the components for 3D microscopy it is necessary under any circumstances to take the precaution measures and observe the warnings contained in the operating instructions The following symbols are used for the warnings Caution Non observance of the safety notes constitutes a hazard for the user Caution Non observance of the safety notes constitutes a hazard for the instru...

Page 5: ...change to 115 is only required for the 3D CONTROL DSA 3 unit For further details see Care and Maintenance on page VIII Cable connections may only be made if the various components are switched off For the video components from KAPPA it is absolutely necessary to use only the connectors listed in the manual Color TV camera for microscopy and 3D images Make sure that the cables are not bent or laid ...

Page 6: ...omponents for 3D microscopy Axiotron 2 Axiotech 100 Axiotech 100 vario Axiovert 100 135 135 M Axiovert 25 CA Axioplan 2 Axiophot 2 3D microscopy is ideal for the following applications in biomedicine micromanipulation patch clamp technique in vitro fertilization examination of endocytosis and xenobiotics examination of phagocytosis injection of nucleotides examination of nerve cells 3D microscopy ...

Page 7: ...shutter 3D illumination shutter is positioned close to the aperture diaphragm and is used to open or close one half of the beam path With the eyepiece version the LCD shutter is controlled via the 3D CONTROL DSA 3 unit The centroid of the illumination beam path is switched in such a way that the object is illuminated alternately from a right and a left stereo angle The eyepiece shutters are contro...

Page 8: ...troid of the illumination beam path is switched in such a way that the object is illuminated alternately from a right and a left stereo angle The camera and the monitor shutters are controlled at the same pulse rate i e each eye only sees the image on its own side right eye right image left eye left image In the monitor version allocation is made via the display of the half images in two perpendic...

Page 9: ...e memory 5 3D illumination shutter 6 Objective 7 Object plane 8 Camera Monitor version in a reflected light microscope 1 Monitor shutter 2 Polarizing glasses 3 Camera 4 Objective 5 Object plane 6 Condenser 7 3D illumination shutter 8 MCU II image memory 9 Control unit for monitor shutter Monitor version in a transmitted light microscope ...

Page 10: ...nation beam path is switched in such a way that the object is illuminated alternately from a right and a left stereo angle The eyepiece shutter and the camera and monitor shutters are controlled at the same pulse rate i e each eye only sees the image on its own side right eye right image left eye left image In the monitor version allocation is made via the display of the half images in two perpend...

Page 11: ... dust using a rubber blower Use a soft optics cleaning cloth Use a natural hair brush degreased in alcohol and dried before The glass plate of the window of the CCD camera can be cleaned using the cleaning buds supplied 2 3 3 Change of voltage and fuses Prior to connection to the line check whether the voltage set on the selector at the rear of the 3D CONTROL DAS 3 unit complies with the line volt...

Page 12: ...illumination shutter Knob of 3D CONTROL DAS 3 control units not set correctly check 3D setting of 3D CONTROL DAS 3 The image flickers pulse rate is not set correctly set 3D VIS on the 3D CONTROL DAS 3 for observation and 3D VIDEO for monitor observation A pseudostereoscopic image is displayed i e heights and depths in the image are exchanged The toggle switch of the 3D CONTROL DAS 3 is not set cor...

Page 13: ... Radio interference suppression in accordance with EN 55011 Class B Insensitivity to noise in accordance with EN 50082 1 The components for 3D microscopy meet the requirements of the EC directive 89 336 EEC and the EMC legislation of November 9 1992 Power supply 3D CONTROL DSA 3 230 VAC 10 50 60 Hz switchable to 115 VAC 10 50 60 Hz Power consumption of the overall system 30 VA minimal 7 VA maximal...

Page 14: ...3D Microscopy Contents Axiovert 100 135 14 B 40 022 4 e 09 98 ...

Page 15: ...of 3D video recordings 29 4 Eyepiece and monitor version 30 4 1 Conversion and adjustment 30 4 2 Observation 31 4 2 1 Eyepiece observation 31 4 2 2 Monitor observation 31 Illustrations Fig 1 Overview 16 Fig 2 Connections for eyepiece version 18 Fig 3 Changing the voltage selector on rear of 3D CONTROL DSA 3 18 Fig 4 Insertion of 3D illumination shutter 19 Fig 5 Insertion of depolarizer 19 Fig 6 3D...

Page 16: ...3D Microscopy Overview Axiovert 100 135 135 M 16 B 40 022 4 e 09 98 1 Components for 3D Microscopy Overview Fig 1 Overview ...

Page 17: ...vert 100 135 135 M 3D depolarizer Axiovert 100 135 135 M Centering telescope Axiovert 100 Cat No 444830 Centering telescope Axiovert 100 Cat No 444830 Centering telescope Axiovert 100 Cat No 444830 6 TV Adapter 60 C 2 3 TV Adapter 60 C 2 3 7 8 9 10 11 12 3D video equipment with monitor shutter including camera monitor and polarizing glasses 3D video equipment with monitor shutter including camera ...

Page 18: ...ith 3D shutter to the EYEPIECE sockets Connect 3D illumination shutter to the ILLUMINATION SHUTTER socket Connection to the line Prior to connection to the line check wether the voltage set on the selector at the rear of the 3D CONTROL DSA 3 unit complies with the line voltage it may be necessary to change from 230 V to 115 V or vice versa Fig 3 Pull the power plug before changing the fuses or the...

Page 19: ... analyzer opening Fig 5 Insertion of depolarizer Insert eyepieces with 3D shutter in the binocular tube Insert 3D illumination shutter in the recess provided in the condenser of the Axiovert 100 135 135 M close to the aperture diaphragm Fig 4 Do not touch the polarizing foil since it can be easily scratched For 3D microscopy remove the DIC prism from the condenser turret if used Insert depolarizer...

Page 20: ...rmines the phase position between the shutters In the STEREO position a stereoscopic upright image is created while the image in the PSEUDO position is pseudostereoscopic vertically reversed Rotary switch for the setting of different modes STEP step mode inclined illumination from the left or right 2D brightness if toggle switch is in STEREO position 3D VIS 3D image eyepiece version 3D VIDEO 3D im...

Page 21: ...ch of the 3D CONTROL DSA 3 to the STEP position Fig 6 Observe the pupil Axiovert 100 remove the eyepiece and insert the centering telescope Axiovert 135 135 M with Bertrand lens in the 4x turret use centering telescope or Bertrand lens to focus on the aperture diaphragm one pupil half will be bright the other dark Fig 7 Move 3D illumination shutter to the side until both pupil halves appear identi...

Page 22: ...llumination shutter against a white background and reset I1 or I2 until both halves display equal brightness There are two ways of adjusting the voltage of the 3D shutters in the eyepieces 3D illumination shutter outside the microscope eyepieces with 3D shutter in the microscope Set the rotary switch of the 3D CONTROL DSA 3 to 2D brightness position View the contrast if required use screwdriver to...

Page 23: ...s not upright exchange the eyepieces Mark the eyepieces and the appropriate plugs with the supplied labels Fig 8 E1 and E2 for the left and right connection plug L and R for the left and right eyepiece To avoid the cables on the eyepieces with 3D shutter to be damaged attach them to the rear without any slack and fix them to the stand using the supplied cable clamps Place the required object on th...

Page 24: ...trol unit CF 15 4 MC S camera CF 15 4 connecting cable to MCU II Line cable Monitor 100 Hz with connecting cable and attached monitor shutter Control unit for monitor shutter with power unit and connecting cable 3D illumination shutter Control and supply cable between MCU II and 3D illumination shutter monitor shutter Polarizing glasses Fig 9 Wiring diagram for monitor version ...

Page 25: ...acket in such a way that the connector sockets are positioned on the bottom right Before connecting the components for the monitor version make absolutely sure to note the following First read the enclosed manual from Kappa entitled Color TV Camera for Microscopy and 3D Images chapter 5 Safety regulations should be observed in particular Cable connections may only be made if the various components...

Page 26: ...ers are recommended Video zoom adapter 0 4x 2x with C mount TV adapter 1x for chip camera with C mount Fig 12 Push 3D illumination shutter in the recess provided in the condenser of the Axiovert 100 135 close to the aperture diaphragm Fig 4 Do not touch the polarizing foil since it can be easily scratched Switch components on in the following order microscope monitor MCU II control unit Running up...

Page 27: ...ttings within the menus Mode Key for menu change Freeze Key for storage of the current image the stored image is constantly made available at the video ports 3 2 2 Basic settings for 3D microscopy USER MENU User 1 RESET MENU Scope LC AB BA corresponds to stereo pseudostereo change White set Set Lock R B 50 Integration Mode Auto Time 20 ms Clock Normal Camera menu Enhance High AGC On AIT Off Exposu...

Page 28: ...ame way as with the eyepiece version remove camera cable from the MCU II or from the camera see page 21 Place the required object on the stage and focus on it Switch beam path to camera TV A 2 dimensional image first appears on the monitor if the image is reversed turn the camera around 180 Wear polarizing glasses The image appears in 3 dimensions on the monitor Place required object on the stage ...

Page 29: ...ing playback Activate TBC Time Base Corrector on the video recorder First activate the Pause Still key then the Record key of the video recorder Use the Pause key to interrupt recording Interrupt playback using the Pause key and end it using the Stop key 3 5 Printing of 3D video recordings Stored 3D video recordings can be printed as video prints Fig 14 Storing the image Set 100 Hz in the MEMORY O...

Page 30: ...Safety regulations should be observed in particular 4 1 Conversion and adjustment Set up the microscope in accordance with the operating instructions of the Axiovert 100 135 135 M Equip the microscope with LD condenser 0 55 Ph1 Ph2 Ph3 DIC DIC Set up the eyepiece version see page 18 Set up the monitor version see page 24 Connect eyepiece and monitor version in accordance with the wiring diagram Fi...

Page 31: ...until optimum 3D contrast is achieved the image appears 3 dimensional through the eyepiece 4 2 2 Monitor observation Switch beam path to Camera TV on the stand Set the 3D VIDEO mode via the rotary switch of the 3D CONTROL DAS 3 control unit Place the required object on the stage and focus on it the image on the monitor is 2 dimensional first If the image is reversed turn the camera around 180 Wear...

Page 32: ...3D Microscopy Eyepiece and monitor version Axiovert 100 135 135 M 32 B 40 022 4 e 09 98 ...