18 Feb 2011,<qsg-wf1> p. 5/8
in "Acquisition Setup" dialogue
•
set "Change focus using" to "Zeiss Focus Drive"
•
choose and set Z-spacing or number of slices
•
set "Auto-focus using" to "Zeiss Focus Drive"
•
choose the lightpath where the Autofocus should take its cues from
o
set
time series
o
in "Acquisition Setup" dialogue: set duration
o
in video toolbar: set intervals
o
set
multiple positions
o
stage needs to be calibrated for this
remove
sample!!!
switch to shortest objective (10x, 2,5x or empty position)!!!
move objective turret to the lowest position!!!
choose "Calibrate Stage" from the "Stage" menu
in the "Acquisition Setup" dialogue set "Change XY using" to "Ludl XY
Stage"
o
"handmade"
positioning
"Video" menu > "Live Video"
"Stage" menu > "Clear All Points"
move to desired point
"Stage" menu > "Add Point"
with "Stage" menu > "Review Points" you can adjust the focus for each of
your points separately
o
automated
positioning
"Video" > "XY Stage
"Stage" > "Make Well Overlay"
"Stage" > "Create Points from Well Overlay"
follow the instructions
Shutdown procedure
•
check if anyone is booked after you within 2 hours
If nobody is booked within two hours:
•
remove specimen
•
clean oil objective lenses with lens tissue
•
Important: close incubation chamber completely (
Æ
protects from dust)
•
update time in Sharepoint
•
secure your data (e.g. copy them to the server)
•
shut down hardware in reverse order from startup
