Zeiss Cell Observer SD, Operating Manual

Page 1: ...l j l pa...

Page 2: ...of technical progress the manual is not covered by an update service Unless expressly authorized forwarding and duplication of this document as well as utilization and communication of its content are...

Page 3: ...Basic Laser Safety Kit 28 2 3 7 Safety Kit for Customer Provided Laser Module 29 2 4 System Overview Cell Observer SD 30 2 5 Suitability of Objectives for the Use 31 2 6 Suitability of Optical Fibers...

Page 4: ...ng the Multi Laser Module On 54 4 3 Switching the System off 55 4 3 1 Turning the Cell Observer SD Off 55 4 3 2 Turning the Multiline Argon Laser Off 55 4 3 3 Turning the Multi Laser Module Off 55 4 4...

Page 5: ...require ments of Directive 98 79 EC In Vitro Diagnostic Medical Device Directive It carries the mark The Cell Observer SD must be disposed in accordance with the WEEE Directive 2002 96 EC The followin...

Page 6: ...ting Manual Installation Manual for Peripherals B 40 650 d Operating Manual for computer system Operating Manual for Camera AxioCam MRm HSm HRm or Photometrics EMCCD Operating Manual for Confocal Scan...

Page 7: ...you begin with procedures for initial start up of the Cell Observer SD Do not expose yourself to the laser beam Do not introduce reflective objects into the beam path Never open shielding or covers at...

Page 8: ...bution strip This is necessary to prevent excessively strong leakage currents refer to section 3 1 2 on page 38 The earth cable which is included in delivery AWG10 green yellow must be used for connec...

Page 9: ...ing the fuses Only use fuses appropriate for the rated current as specified The use of makeshift fuses and short circuiting of the fuse holders are not permitted On completion of work for installation...

Page 10: ...enty of water for at least five minutes See a specialized physician in case of persisting irritation Proper disposal Do not drain Immersol 518 F into surface water or sewer system Avoid touching the h...

Page 11: ...vice technicians If one fiber end is not firmly connected to the fiber mount laser class 3B radiation will emerge Non observance involves the risk of eye damage CAUTION The cover Fig 1 1 3 at the join...

Page 12: ...n carrier and HAL lamp CAUTION Only Carl Zeiss service personnel are allowed to release the clamping screw Fig 1 2 1 If the lamp housing is removed laser class 3B radiation will emerge Non observance...

Page 13: ...low all warning and information labels that are affixed to the Cell Observer SD and the Axio Observer microscope Please check that all labels shown below are actually in place Contact Carl Zeiss Micro...

Page 14: ...NOTES ON INSTRUMENT SAFETY Carl Zeiss Safety Instructions Cell Observer SD 14 423638 7044 001 M60 2 0021 e 01 2009 Fig 1 4 Warning and information labels at the Cell Observer SD...

Page 15: ...NOTES ON INSTRUMENT SAFETY Cell Observer SD Safety Instructions Carl Zeiss M60 2 0021 e 01 2009 423638 7044 001 15 Fig 1 5 Warning and information labels at the filter wheel...

Page 16: ...ES ON INSTRUMENT SAFETY Carl Zeiss Safety Instructions Cell Observer SD 16 423638 7044 001 M60 2 0021 e 01 2009 Caution Fuse in N Line too Fig 1 6 Warning and information labels at the multi laser mod...

Page 17: ...Cell Observer SD Safety Instructions Carl Zeiss M60 2 0021 e 01 2009 423638 7044 001 17 Fig 1 7 Warning and information labels at the laser module variant 1 Fig 1 8 Warning and information labels at...

Page 18: ...NOTES ON INSTRUMENT SAFETY Carl Zeiss Safety Instructions Cell Observer SD 18 423638 7044 001 M60 2 0021 e 01 2009 Fig 1 9 Warning and information labels at the SVB 1 optional component...

Page 19: ...g from faulty operation negligence or any other meddling with the instrument particularly the removal or replacement of instrument components or the use of accessories from other manufacturers Any suc...

Page 20: ...ight source Alternatively a multi line Ar laser may be selected One or two CCD cameras perform real time image capturing The spinning disc unit should only be operated when combined with an Axio Obser...

Page 21: ...ith a special tandem disc setup The Nipkow disc acts in unison with a preceding second mechanically coupled disc It contains micro lenses to exactly focus and direct the incident bundle of excitation...

Page 22: ...objective lenses for example Plan Apochromat 63x 1 4 Oil PC with working memory not less than 512 MB Monitor for image display AxioCam MRm AxioCam HRm AxioCam HSm digital camera or Photometrics EMCCD...

Page 23: ...000 spinning disc unit Confocal scanner with one camera port filter wheel and variable spinning disc rotation speed from 1500 to 5000 rpm A CSU X1 control box is available for upgrading a CSU X1M 1800...

Page 24: ...NSTRUMENT DESCRIPTION Carl Zeiss Instrument Description and Main Features Cell Observer SD 24 423638 7044 001 M60 2 0021 e 01 2009 Fig 2 2 Cell Observer SD left und Cell Observer SD with Dual Cam righ...

Page 25: ...01 2009 423638 7044 001 25 2 3 2 CSU X1 Control Box The CSU X1 control box controls the scanning speed and the filter wheel actions For compatibility issues you should consult the system overview in s...

Page 26: ...ptical fiber cable links the laser module with the spinning disc unit Fig 2 4 Multi laser module The laser lines are controlled by means of the laser controller included in the delivery which is conne...

Page 27: ...B 1 operating manual Fig 2 6 Signal Distribution Box SVB 1 2 3 5 Filter Wheel The filter wheel can be configured with a maximum of six filters These are individually moved into and out of the optical...

Page 28: ...tand Fig 3 9 using a 15 pole sub D connector Fig 2 8 1 The four jack terminals of this distribution box Fig 2 8 2 are available for connection to the laser protection cover or laser protection incubat...

Page 29: ...ection cable Fig 2 9 3 The laser beam must be completely blocked by the shutter blade Fig 2 9 1 in its lower de energized position Fig 2 9 Laser shutter converter board and connection cable of safety...

Page 30: ...INSTRUMENT DESCRIPTION Carl Zeiss System Overview Cell Observer SD Cell Observer SD 30 423638 7044 001 M60 2 0021 e 01 2009 2 4 System Overview Cell Observer SD Fig 2 10 System overview...

Page 31: ...tance of 250 m from each other The pinhole diameter is optimized for strongly magnifying objective lenses with a high numerical aperture 100x 1 4 or similar Using the CSU X1 with weakly magnifying obj...

Page 32: ...finite Focus controller 250 mm x 95 mm x 220 mm HXP120 130 mm x 210 mm x 290 mm Weight CSU X1M 1800 5000 spinning disc unit 7 5 kg CSU X1M 1800 5000 Dual Cam spinning disc unit 9 5 kg CSU X1A 5000 spi...

Page 33: ...Change of instrument voltage setting is not necessary Line frequency 50 60 Hz Power consumption with internal power supply units Axio Observer Z1 with external VP231 power supply unit 280 VA Power su...

Page 34: ...lasers will be solely responsible for overall laser safety of the Cell Observer SD system They must check for further required laser safety precautions and validity of all laser warning labels affixed...

Page 35: ...ss B Noise immunity in accordance with DIN EN 61326 1 Control Box CSU X1 Line voltage 100 to 240 V 10 Change of instrument voltage setting is not necessary Line frequency 50 60 Hz Maximum power consum...

Page 36: ...1 IEC 61010 1 under consideration of CSA and UL directives Overvoltage category II Radio interference suppression in accordance with EN 55011 class B Noise immunity in accordance with DIN EN 61326 1 P...

Page 37: ...of Carl Zeiss MicroImaging GmbH or persons duly authorized to do so by the Manufacturer Intended and conforming use provided the Cell Observer SD can be commissioned and operated by Customer personnel...

Page 38: ...ps included in delivery for connection of the system components refer to Fig 3 2 The multiple socket outlets must not be connected in series The multi laser module must be grounded Delivery includes a...

Page 39: ...24 h per day whether system is operational or in power off state 2 Operation at reduced power T 10 C to 35 C with all operating details modified against 1 3 Warm up time 1 h 2 h for high precision and...

Page 40: ...om those quoted herein will be deemed as non conforming use In particular no warranty can be assumed by Carl Zeiss MicroImaging GmbH regarding laser safety issues Because the system includes highly ma...

Page 41: ...ol 518 F ready for this purpose Read in any case the Immersol 518 F safety data sheet Turn the lens turret into an empty position 3 4 Height Setting of CSU X1 Spinning Disc Unit Before the CSU X1 spin...

Page 42: ...tallation is identical for both camera adapter types Release the two fixing screws Fig 3 4 2 at the camera adapter then take the adapter piece Fig 3 4 1 out Remove anti dust caps from adapter end piec...

Page 43: ...DC IN Fig 3 6 1 Plug the interlock connector into terminal jack Ext Control Interlock Fig 3 6 2 The shutter can now be manually opened and closed by pressing the Shutter button Fig 4 1 1 3 6 2 Connec...

Page 44: ...Box Connect terminal RS232C1 Fig 3 8 2 of the CSU X1 control box to the computer Plug the control cable of the CSU X1 spinning disc unit into terminal CSU Fig 3 8 1 of the CSU X1 control box Connect t...

Page 45: ...be able to open For operation with your own laser the laser shutter of the TIRF laser safety kit for Customer provided laser modules 432921 0000 000 must be mounted in such a position that it will blo...

Page 46: ...door interlock switch Fig 3 9 7 You are advised to contact your Laser Safety Officer for connection of the door switch Unplug the green plug Fig 3 9 7 from the distribution box for laser safety Loosen...

Page 47: ...her wavelengths are used the Cell Observer SD protection system must be reviewed for protective effect Notify your Laser Safety Officer before proceeding with the initial system start up Valid nationa...

Page 48: ...t into locked position Laser shutter open Eyepiece shutter closed Remote connector detached from distribution box for laser safety signals Laser shutter closed Eyepiece shutter open Remote connector p...

Page 49: ...int appears on opaque laser protection cover Swing transmitted light arm out of working position Laser shutter closes and laser light point on opaque laser protection cover extinguishes Swing transmit...

Page 50: ...ure to prevent exposure to radiation from such lasers Do not look directly into the laser beam 4 1 Control and Functional Components 4 1 1 CSU X1 Spinning Disc Unit 1 Shutter hbutton 4 Push rod for ca...

Page 51: ...ain power switch 2 Automatic circuit breaker switch Fig 4 2 Control and functional components of CSU X1 control box rear side view 4 1 3 Multi Laser Module 1 Line power inlet port 3 Main power switch...

Page 52: ...ect a suitable emission filter Refer to section 4 6 for details regarding conversion to another emission filter Turn the key switch at the spinning disc unit Fig 4 1 2 to position ON The green control...

Page 53: ...energy too high Fig 4 5 Control unit of multiline argon laser 1 Power supply unit of 100 mW laser 2 Key switch 3 On Off switch Fig 4 6 Power supply unit of multiline argon laser Nominal laser power wi...

Page 54: ...e session Turn the key switch Fig 4 8 1 of the laser con troller into position ON To turn a desired laser line on press the related button Fig 4 8 2 The corresponding red control LED Fig 4 8 3 will li...

Page 55: ...king session potentiometer Fig 4 5 2 in left stop position Transfer the laser power controller into Standby mode by flipping the toggle switch Fig 4 5 3 down Turn the key switch Fig 4 6 2 into positio...

Page 56: ...u to switch between two camera ports Pull the push rod out Fig 4 1 4 to switch to the upper camera port If a secondary color splitter is installed the short wave emission light portion will be directe...

Page 57: ...g 4 10 3 adapter in a stable position Loosen the four fixing screws Fig 4 10 1 of the camera adapter Take the camera adapter Fig 4 10 3 off Insert the EMCCD camera adapter into the spin ning disc unit...

Page 58: ...to the spinning disc unit until mechanical stop po sition Tighten the clamping screw 2 5 Allen key Fig 4 12 1 Connect the filter wheel to the CSU X1 control box refer to section 3 6 2 Exchange of filt...

Page 59: ...ians specifically authorized to perform such work To switch between the two camera channels move the push rod in or out as necessary Fig 4 1 4 The lateral camera channel will be active when the push r...

Page 60: ...with a mild detergent Do not allow liquid to penetrate into the inner system space Refrain from the use of solvents Use a non shedding piece of cloth to wipe the surfaces clean and dry Cleaning of gla...

Page 61: ...losed Open shutter by pressing the Shutter button Fig 4 1 1 CSU X1 control box not ON Turn CSU X1 control box on refer to section 4 2 1 Rotation speed of spinning disc unit cannot be set CSU X1 not co...

Page 62: ...mall screwdriver for convenience Extract fuses from the fuse holder and replace them with new fuses of type T 1A H 250 V Insert fuse holder into the fuse compartment until mechanical stop position Fig...

Page 63: ...e the fuse compartment Re connect main power plug 5 5 Replacement of HAL Halogen Lamp The halogen lamp of the HAL illuminator may not be replaced by anyone other than service personnel of Carl Zeiss M...

Page 64: ...ce personnel of Carl Zeiss MicroImaging GmbH or specifically authorized expert technicians In order to keep your spinning disc unit in an optimally adjusted and faultlessly operating condition for an...

Page 65: ...titut f r Normung EN European Standard EMV Electromagnetic Compatibility HBO Mercury Vapor Short Arc Lamp for Fluorescence IEC International Electrotechnical Commission IP Internal Protection Standard...

Page 66: ...pinning disc unit 50 Multi laser module 51 CSU X1 control box 25 44 51 CSU X1 spinning disc unit 50 D Description 20 Dimensions 32 Distribution box for laser safety Connection 45 Dual Cam option 56 59...

Page 67: ...O Objectives 31 Operation 32 50 Optical fibers 31 S Safety 5 Safety check 48 Safety kit 29 Service 64 Service interfaces 11 Signal distribution box SVB 1 27 Start up 37 Switching the system off 55 Sw...

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