Iron Man User Guide v. 1.4
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Using the dichroic in position (3) we sent all light greater than 543 to channel 1. This is further
selected for using the (7) emission filter. For rhodamine, we would use a LP615.
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If you want to image all three channels in the same track, select the track and check on the three
channels. It is important to note the overlap in emission spectra. In this example, it is possible
that some of the DAPI emission will show up in the FITC channel and some FITC emission will show
up in the Rhodamine channel. If your fluorophores are spectrally separated enough, you can use
multiple channels on the same track. In this example you could have DAPI and Rhodamine on one
track and image FITC in a separate track
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If you want multiple tracks, click the add track button and check on the channel for each.
