Iron Man User Guide v. 1.4
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The software will open to the ocular tab:
Online mode will allow you to view your sample through the oculars and offline mode allows you to view the
sample on the PC screen.
This window will allow you to control/view the following
microscope positions:
1)
Halogen bulb for Brightfield viewing
2)
Aperture
3)
Specimen / stage
4)
Objective – You can change the objective here or using
the buttons on the side of the microscope
USE CAUTION WHEN SWITCHING BETWEEN AIR AND
OIL OBJECTIVES
5)
Reflector cubes – These should be changed using the
shortcut configurations above
6)
Hg bulb shutter
7)
Hg bulb
8)
Lens
To minimize photobleaching of your sample, close the
Fluorescence Shutter whenever you are not looking
through the eyepieces.
To look at your slide:
1)
Load the slide, coverslip down, onto the slide holders on the stage (these can be adjusted manually).
Please be sure that your slide is clean and dry – do not get wet mounting media or condensation on
the stage.
2)
Position the slide over the desired objective.
-
If you are using an air objective, start at the lowest focus position.
-
If you are using oil immersion, place a small drop of oil on the slide before placing it on the
microscope.
Do not switch back to an air objective
. Turn the focus knob up until the objective
lens is just touching the oil.
3)
Click into online mode, and choose the appropriate reflector cube configuration (#5) and open the
fluorescence shutter.
4)
Looking through oculars, carefully focus on your sample and center on the structure you want to
image. Done? Close the fluorescence shutter and switch to offline mode.
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