Iron Man User Guide v. 1.4
6
The Acquisition Tab:
1)
At the top
– select any options you want for your
experiment (Z-series, time series)
2)
In the Setup Manager window
– turn on the appropriate
lasers for your experiment. You do not need to turn on
lasers you will not be using.
-
Argon/2
: Select Standby from dropdown to warm up the
laser. The status will read “Ready” when it is done.
Choose On. Open the Laser Properties window by
clicking on the arrow, and adjust laser output [%] to
40%.
Do not go over 40% output or over 6.1 A.
Do not
keep changing this Output % - set it for your imaging
session and leave it alone. Keeping the laser at 6.0 A is
done to assure that the laser output is at a stable and
reproducible intensity when using the confocal
scanhead.
-
Diode
: Select “Standby” from dropdown to warm up
the laser. The status will read “Ready” when it is
done, and only then can you choose “On”
-
HeNe
– choose “on” if you need these lasers
