Zeiss LSM 510 Inverted, Brief Operating Manual

 
 
8  

07/05 

Configuring the beam path and lasers 

• 

Click on the 

LSM

 button in the 

Acquire

subordinate toolbar for laser scanning. 

Choosing the configuration 

Single Track   

− 

Use for 

single

, double and triple labeling; 

simultaneous scanning only 

− 

Advantage: faster image acquisition 

− 

Disadvantage: cross talk between channels 

Multi Track 

− 

Use for double and triple labeling; sequential 
scanning, line by line or frame by frame 

− 

Advantage: when one track is active, only 
one detector and one laser is switched on. 
This reduces cross talk. 

− 

Disadvantage: slower image acquisition 

• 

Click on the 

Config 

button in the 

Acquire

 subordinate toolbar to 

open the 

Configuration Control

window. 

The 

Configuration Control

 window 

appears (Fig. 12). 

Setting for single track configuration in Channel Mode 

• 

Select 

Channel Mode

 if necessary (Fig. 12). 

• 

Click on the 

Single Track 

button in the 

Configuration Control

 window (Fig. 12). 

• 

Click on the 

Descanned

 button if necessary. 

The 

Beam Path and Channel Assignment 

panel of the 

Configuration Control 

window displays the 

selected track configuration which is used for the scan procedure.  

• 

You can change the settings of this panel using the following function elements: 

Activation / deactivation of the excitation wavelengths (check box) and setting of 
excitation intensities (slider). Open the 

Laser Control 

window via the 

Laser

 button. 

Selection of the main dichroic beam splitter (HFT) or secondary dichroic beam splitter 
(NFT) position through selection from the relevant list box. 

Selection of an emission filter through selection from the relevant list box. 

Activation / deactivation (via check box) of the selected channel (Ch 1-4, monitor diode 
ChM, META detectors ChS1-8, transmission ChD) for the scanning procedure by 
assigning an existing color icon or defining a new one.  

Fig. 12 

Configuration Control window for 
Single Track