QUICKSTART
LSM
510
Carl Zeiss
Quick Start in the Expert Mode
LSM 510 META
4-14
B 45-0008 e 10/02
4.3.2
Set the Microscope
This step is used to set:
−
microscope objective
−
specimen position
−
specimen focus
4.3.2.1
Axioplan 2 imaging
MOT
•
Click on the
Acquire
button in the toolbar of the
Main
menu.
•
Move the tube slider on the microscope to the
VIS
position.
•
Click on the
Micro
button in the
Acquire
subordinate toolbar.
−
The
Axioplan Control
window appears on
the screen.
•
Put the specimen on the stage - make sure the
specimen is mounted securely and flat. For tests
use the supplied Convallaria specimen.
You can view the specimen in either fluorescence
(reflected light) or transmitted light.
•
To view the specimen in transmitted light, set
the
Reflector Turret
position to
None
and
activate the
Transmitted Light
panel by
clicking on the
Transmitted Light
button.
Select the
On
button and control the intensity
by the slider.
•
Select an objective with low magnification by
clicking in the
Objective
panel of the
Axioplan Control
window.
•
Set the microscope to KÖHLER illumination
manually (see Axioplan 2 imaging
MOT
operat-
ing manual).
•
Select the specimen area to be examined by
moving the XY-stage and focus exactly on the
selected area.
•
Close the
Axioplan Control
window. Move
the tube slider on the microscope to the
LSM
position.
Fig. 4-12
Axioplan Control window
Summary of Contents for LSM 510 META
Page 1: ...LSM 510 and LSM 510 META Laser Scanning Microscopes Operating Manual Release 3 2...
Page 8: ...INTRODUCTION LSM 510 Carl Zeiss LSM 510 META VIII B 45 0008 e 10 02...
Page 10: ...NOTES ON DEVICE SAFETY LSM 510 Carl Zeiss Contents LSM 510 META 1 2 B 45 0008 e 10 02...
Page 20: ...NOTES ON DEVICE SAFETY LSM 510 Carl Zeiss LSM 510 META 1 12 B 45 0008 e 10 02...
Page 22: ...LSM 510 SETUP REQUIREMENTS LSM 510 Carl Zeiss Contents LSM 510 META 2 2 B 45 0008 e 10 02...
Page 52: ...QUICKSTART LSM 510 Carl Zeiss Contents LSM 510 META 4 2 B 45 0008 e 10 02...
