OPERATION IN EXPERT MODE
LSM 510
DuoScan
Carl Zeiss
Acquire Menu
LSM 510 META
DuoScan
4-38
B 45-0021 e
03/06
4.5 Acquire
Menu
For preparing and acquiring a scanning image, it is recommended to call up and use the tools of the
subordinate toolbar in the following order:
•
Conventional microscope setting.
•
Laser setting.
•
Configuring the optical system for the Scanning Mode.
•
Setting of scan parameters.
•
EditROI
permits up to 99 regions within a frame to be defined and scanned.
•
TimeSeries
permits user-specific time series to be selected for the scan procedure.
•
The
EditBleach
function is used to bleach a defined, freely selectable area within the scanning field.
•
Upon selecting
Stage
you can set the focus (Z coordinate) and the Z step size between successive
slices. If the optional, motorized X/Y-stage is connected, the X and Y-positions of the sample can also
be selected.
•
The
VIS
,
TV
and
LSM
buttons switch the beam path and indicate which beam path has been set in
the binocular tube of the microscope (VIS for viewing, TV for camera observation, LSM for laser
operation with monitor observation).
For the scanning process, the
LSM
button in the toolbar subordinate to the
Acquire
item must
be activated.
•
In the
Main
menu toolbar, click on
Acquire
.
−
This opens another, subordinate toolbar in the
Main
menu.
Fig. 4-25
Acquire menu
